Extracellular Potassium and Calcium Activities in the Mammalian Spinal Cord, and the Effect of Changing Ion Levels on Mammalian Neural Tissues

Somjen, George G.; Dingledine, Raymond; Connors, Barry W.; Allen, Brian W.

doi:10.1007/978-1-4615-9224-2_15

Cited by 8 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to determine Cli for varying Ko, we obtained Em from a study that examined the dependence of Em on Ko (Somjen et al, 1981) -the dashed line in Fig. 5C is adapted from that study and demonstrates the change in Em with varying Ko e and calculated Cli for specific Ko concentrations using the formula above and the previously determined value of the factor a.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Could tuning of the inhibitory tone involve graded changes in neuronal chloride transport?

2015

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…a: KCC2-WT; b: T906A T1007A; c: T1052A; d: T906A T1007A T1052A. The dashed line shows the membrane potential as a function of increasing [K þ ] o (adapted fromSomjen et al, 1981), the thick line indicates the E GABA where the driving force for KCC2 becomes zero.…”

mentioning

confidence: 98%

Could tuning of the inhibitory tone involve graded changes in neuronal chloride transport?

2015

View full text Add to dashboard Cite

“…is now well documented for a wide variety of excitable cells. These cells include the squid giant axon (Curtis & Cole, 1942;Hodgkin & Huxley, 1945), neurones in the leech nervous system (Schlue & Deitmer, 1984), the vertebrate node of Ranvier (Huxley & Stampfli, 1951 a, b), and mammalian dorsal root ganglion cells (Somjen, Dingledine, Connors & Allen, 1981).…”

Section: Introductionmentioning

confidence: 99%

“…Although there has been considerable interest in the effects of extracellular accumulation of K+ ions on neurones in the central nervous system (CNS) (see reviews by Somjen et al 1981;Sykova', 1983) the only studies on the effects of different extracellular ionic concentrations on the membrane potential of CNS neurones have been on amphibian spinal motoneurones (Sykova' & Orkand, 1980) and in cultured mouse spinal cord neurones (Hosli, Andres & Hosli, 1972). The experiments described here were carried out on spinal motoneurones of the young rat using an in vitro preparation (Otsuka & Konishi, 1974;Fulton, Miledi & Takahashi, 1980).…”

Section: Introductionmentioning

confidence: 99%

The dependence of motoneurone membrane potential on extracellular ion concentrations studied in isolated rat spinal cord.

Forsythe

Redman

1988

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Intracellular recordings from ninety-nine motoneurones have been made in an in vitro hemisected spinal cord preparation. Their mean resting membrane potential in normal artificial cerebrospinal fluid (CSF) was -71 +0 5 mV (+ S.E.M.). The mean amplitude of the action potential was 84-0 + 1-4 mV (n = 50), and the mean input conductance was 101 + 7 nS (n = 49).2. Both membrane potential and input conductance were sensitive to changes in 9. The input conductances for Na+, Cl-and K+ at the resting potential were calculated. After allowing for a microelectrode leak conductance, the relative input conductances were 9Na/gK = 0-13 and 9C1/gK = 0-25. * Present address: Department of Physiology, Leicester University, Leicester LEI 7RH.

show abstract

“…Indeed, this assembly has been used when the measurement takes place in excitable tissues, because the effect caused by the locally generated electrical potentials were thus minimized. 21 Although this is not true in all cases, for instance, nerve spike potentials cannot simply be canceled, 22 it seemed worth to examine in more detail the applicability of these assemblies in corrosion science.…”

mentioning

confidence: 99%

Double Barrel Microelectrode Assembly to Prevent Electrical Field Effects in Potentiometric SECM Imaging of Galvanic Corrosion Processes

Filotás

Fernández-Pérez

Nagy

et al. 2018

J. Electrochem. Soc.

View full text Add to dashboard Cite

There is great interest in elucidating the corrosion mechanism of magnesium, and different experimental methods and techniques are explored with this purpose. Among the scanning probe techniques, scanning electrochemical microscopy (SECM) is delivering some promising results in recent years. In particular, the use of ion selective microelectrodes (ISME) as SECM sensing probes allow monitoring of the temporal and spatial distribution of different ionic species related to the corrosion reactions. However, a serious disturbance in the measured potential can be observed when it comes to galvanic coupling or polarization of the samples. This work explores the factors that affect the magnitude of the electrical field effects associated with the galvanic coupling, and describes an experimental arrangement for potentiometric SECM able to avoid the unwanted contribution of the potential field. The performance of a double barrel electrode assembly comprising an ion selective microelectrode and an internal reference electrode was compared to that of a conventional single barrel ISME in order to establish its applicability for the investigation of corrosion systems presenting electrical field distributions.

show abstract

Extracellular Potassium and Calcium Activities in the Mammalian Spinal Cord, and the Effect of Changing Ion Levels on Mammalian Neural Tissues

Cited by 8 publications

References 41 publications

Could tuning of the inhibitory tone involve graded changes in neuronal chloride transport?

Could tuning of the inhibitory tone involve graded changes in neuronal chloride transport?

The dependence of motoneurone membrane potential on extracellular ion concentrations studied in isolated rat spinal cord.

Double Barrel Microelectrode Assembly to Prevent Electrical Field Effects in Potentiometric SECM Imaging of Galvanic Corrosion Processes

Contact Info

Product

Resources

About