Extracellular polymeric substances responsible for bacterial adhesion onto solid surface

Tsuneda, Satoshi; Aikawa, Hirotoshi; Hayashi, Hiroshi; Yuasa, Atsushi; Hirata, Akira

doi:10.1016/s0378-1097(03)00399-9

Cited by 470 publications

(246 citation statements)

References 28 publications

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“…However, NH 4 þ -N was removed to a greater extent by the CAUF cake layer after a long operation time (as indicated in Stage 1), and this could be attributed to the development of a higher concentration of microorganisms with increasing run time. Tsuneda et al [58] reported that sludge adhesion was enhanced by polymeric interactions when the bound EPS content increased. Sludge with abundant bound EPS tends to adhere to the membrane surface and form a stable structure, which could prevent the cake layer from being washed away.…”

Section: Discussionmentioning

confidence: 99%

Investigation of pre-coagulation and powder activate carbon adsorption on ultrafiltration membrane fouling

et al. 2014

Journal of Membrane Science

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a b s t r a c tPre-coagulation by alum before ultrafiltration without (CUF) and with (CAUF) powdered activated carbon (PAC) addition was investigated to explore the influence of PAC on membrane fouling. It was found that the continuous addition of a low dosage of PAC reduced membrane fouling for operational run periods o20 days, both for reversible and for irreversible components of fouling. The presence of PAC increased the removal of dissolved organic matter (DOM), particularly proteinaceous substances, and some fractions of humic-type substances. For a longer operation time (420 days) an increase in mainly reversible fouling was evident and was attributed to microorganism growth in the cake layer, as indicated by the enhanced concentrations of extracellular polymeric substances, such as polysaccharides and proteins. A process of crystallization of the coagulant floc in the cake layer with increasing operation time was believed to cause desorption of DOM from primary coagulation particles and a decrease in their size, and consequently a higher density of cake layer. PAC absorption of desorbed DOM within the cake layer, and residual DOM after coagulation, reduced DOM reaching the membrane surface and the extent of subsequent internal membrane fouling.Crown

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Section: Discussionmentioning

confidence: 99%

Investigation of pre-coagulation and powder activate carbon adsorption on ultrafiltration membrane fouling

et al. 2014

Journal of Membrane Science

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“…Nonetheless, our observations support the conclusion that the psl cluster encodes gene products that are required for mediating cell-surface and cell-cell interactions. Initial attachment requires that a repulsion barrier between a negatively charged bacterial cell and a surface must be overcome (55,56). Different exopolysaccharides, such as those from Vibrio sp., acidic polysaccharides of marine pseudomonads, and Staphylococcus epidermidis PIA and PS/A clearly promote adherence (6,18,32,35,65).…”

Section: Discussionmentioning

confidence: 99%

Identification ofpsl, a Locus Encoding a Potential Exopolysaccharide That Is Essential forPseudomonas aeruginosaPAO1 Biofilm Formation

et al. 2004

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Bacteria inhabiting biofilms usually produce one or more polysaccharides that provide a hydrated scaffolding to stabilize and reinforce the structure of the biofilm, mediate cell-cell and cell-surface interactions, and provide protection from biocides and antimicrobial agents. Historically, alginate has been considered the major exopolysaccharide of the Pseudomonas aeruginosa biofilm matrix, with minimal regard to the different functions polysaccharides execute. Recent chemical and genetic studies have demonstrated that alginate is not involved in the initiation of biofilm formation in P. aeruginosa strains PAO1 and PA14. We hypothesized that there is at least one other polysaccharide gene cluster involved in biofilm development. Two separate clusters of genes with homology to exopolysaccharide biosynthetic functions were identified from the annotated PAO1 genome. Reverse genetics was employed to generate mutations in genes from these clusters. We discovered that one group of genes, designated psl, are important for biofilm initiation. A PAO1 strain with a disruption of the first two genes of the psl cluster (PA2231 and PA2232) was severely compromised in biofilm initiation, as confirmed by static microtiter and continuous culture flow cell and tubing biofilm assays. This impaired biofilm phenotype could be complemented with the wild-type psl sequences and was not due to defects in motility or lipopolysaccharide biosynthesis. These results implicate an as yet unknown exopolysaccharide as being required for the formation of the biofilm matrix. Understanding psl-encoded exopolysaccharide expression and protection in biofilms will provide insight into the pathogenesis of P. aeruginosa in cystic fibrosis and other infections involving biofilms.

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“…In contrast, studies of EPS extracted from activated sludges show a majority of proteins (McSwain et al 2005;Ge et al 2007;Park and Novak 2007). In smaller quantities, humic-like substances (Frølund et al 1995), uronic acids (Tsuneda et al 2003), and nucleic acids (Zhang et al 1999) are also EPS constituents. Nucleic acids are released after cell lysis.…”

Section: Introductionmentioning

confidence: 99%

Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

d’Abzac

Bordas

Hullebusch

et al. 2009

Appl Microbiol Biotechnol

271

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The characteristics of the extracellular polymeric substances (EPS) extracted with nine different extraction protocols from four different types of anaerobic granular sludge were studied. The efficiency of four physical (sonication, heating, cationic exchange resin (CER), and CER associated with sonication) and four chemical (ethylenediaminetetraacetic acid, ethanol, formaldehyde combined with heating, or NaOH) EPS extraction methods was compared to a control extraction protocols (i.e., centrifugation). The nucleic acid content and the protein/ polysaccharide ratio of the EPS extracted show that the extraction does not induce abnormal cellular lysis. Chemical extraction protocols give the highest EPS extraction yields (calculated by the mass ratio between sludges and EPS dry weight (DW)). Infrared analyses as well as an extraction yield over 100% or organic carbon content over 1 gg −1 of DW revealed, nevertheless, a carry-over of the chemical extractants into the EPS extracts. The EPS of the anaerobic granular sludges investigated are predominantly composed of humic-like substances, proteins, and polysaccharides. The EPS content in each biochemical compound varies depending on the sludge type and extraction technique used. Some extraction techniques lead to a slightly preferential extraction of some EPS compounds, e.g., CER gives a higher protein yield.

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Extracellular polymeric substances responsible for bacterial adhesion onto solid surface

Cited by 470 publications

References 28 publications

Investigation of pre-coagulation and powder activate carbon adsorption on ultrafiltration membrane fouling

Investigation of pre-coagulation and powder activate carbon adsorption on ultrafiltration membrane fouling

Identification ofpsl, a Locus Encoding a Potential Exopolysaccharide That Is Essential forPseudomonas aeruginosaPAO1 Biofilm Formation

Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

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