Extracellular polymeric substances of acidophilic microorganisms play a crucial role in heavy metal ions adsorption

Li, M.; Deng, Xiaoying; Sun, Wei; Hu, Liang; Zhong, Hong; He, Zhiguo; Xiong, Dan

doi:10.1007/s13762-021-03352-9

Cited by 16 publications

(5 citation statements)

References 56 publications

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“…The absorption peak at 1624 cm −1 is the stretching of the protein-related amide I band, which is conducive to biological flocculation. This is also in line with the report stating that the EPS produced by bacteria gets attached to the mineral surface (Li et al 2022) interaction between bacteria and minerals. After bioleaching, the spectral band of 467, 503 and 626, 1078 and 1427 cm −1 were changed, compared to the primary phosphate rock.…”

Section: Fourier Transform Infrared Spectroscopy Analysissupporting

confidence: 92%

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Bioleaching of rare-earth elements from phosphate rock using Acidithiobacillus ferrooxidans

Tian

Song

et al. 2022

Letters in Applied Microbiology

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Phosphate rock containing rare‐earth elements (REEs) is considered one of the most promising potential secondary sources of REEs, as evidenced by large tonnages of phosphate rock mined annually. The bioleaching of REEs from phosphate rock using Acidithiobacillus ferrooxidans was done for the first time in this study, and it was found to be greater than abiotic leaching and was more environmentally friendly. The result showed that the total leaching rate of REEs in phosphate rock was 28·46% under the condition of 1% pulp concentration and pH = 2, and the leaching rates of four key rare earths, Y, La, Ce and Nd, were 35·7, 37·03, 27·92 and 32·53% respectively. The bioleaching process was found to be accomplished by bacterial contact and Fe2+ oxidation. The blank control group which contained Fe2+ was able to leach some of the rare earths, indicating that the oxidation of Fe2+ may affect the leaching of rare earths. X‐ray diffraction analysis showed that the minerals were significantly altered and the intensity of the diffraction peaks of dolomite and apatite decreased significantly after microbial action compared to the blank control, and it was observed that bacteria adhere to the mineral surface and the minerals become smooth and angular after bioleaching by scanning electron microscope, indicating that bacteria have a further effect on the rock based on Fe2+ oxidation. Finally, Fourier transform infrared spectroscopy and three‐dimensional excitation‐emission matrix fluorescence spectra analysis showed that extracellular polymeric substances participate in the bioleaching process.

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Section: Fourier Transform Infrared Spectroscopy Analysissupporting

confidence: 92%

“…This is also in line with the report stating that the EPS produced by bacteria gets attached to the mineral surface (Li et al . 2022), mediating the interaction between bacteria and minerals. After bioleaching, the spectral band of 467, 503 and 626, 1078 and 1427 cm −1 were changed, compared to the primary phosphate rock.…”

Section: Resultsmentioning

confidence: 99%

Bioleaching of rare-earth elements from phosphate rock using Acidithiobacillus ferrooxidans

Tian

Song

et al. 2022

Letters in Applied Microbiology

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“…The weak absorption at 2958.39 cm −1 in the unclassified Halolamina sp. strain NRS_38 ( Figure 4 A) is related to the C–H stretching of the –CH 3 group in fatty acids, while the peaks around 2927.30 and 2925.87 cm −1 are due to symmetric stretching of the C-H bond in CH 2 groups [ 51 , 52 ]. The sharp peaks identified around 1628.98 and 1629.28 cm −1 could be related to the carbonyl (-C=O) group on amides and related to proteins [ 53 ] or may be associated with the –CONH– group in amino sugars and proteins [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Polyhydroxybutyrate, PHB, Synthesized by Newly Isolated Haloarchaea Halolamina spp.

et al. 2022

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This work aims to characterize the haloarchaeal diversity of unexplored environmental salty samples from a hypersaline environment on the southern coast of Jeddah, Saudi Arabia, looking for new isolates able to produce polyhydroxyalkanoates (PHAs). Thus, the list of PHA producers has been extended by describing two species of Halolamina; Halolamina sediminis sp. strain NRS_35 and unclassified Halolamina sp. strain NRS_38. The growth and PHA-production were investigated in the presence of different carbon sources, (glucose, sucrose, starch, carboxymethyl cellulose (CMC), and glycerol), pH values, (5–9), temperature ranges (4–65 °C), and NaCl concentrations (100–350 g L−1). Fourier-transform infra-red analysis (FT-IR) and Liquid chromatography–mass spectrometry (LC-MS) were used for qualitative identification of the biopolymer. The highest yield of PHB was 33.4% and 27.29% by NRS_35 and NRS_38, respectively, using starch as a carbon source at 37 °C, pH 7, and 25% NaCl (w/v). The FT-IR pattern indicated sharp peaks formed around 1628.98 and 1629.28 cm−1, which confirmed the presence of the carbonyl group (C=O) on amides and related to proteins, which is typical of PHB. LC-MS/MS analysis displayed peaks at retention times of 5.2, 7.3, and 8.1. This peak range indicates the occurrence of PHB and its synthetic products: Acetoacetyl-CoA and PHB synthase (PhaC). In summary, the two newly isolated Halolamina species showed a high capacity to produce PHB using different sources of carbon. Further research using other low-cost feedstocks is needed to improve both the quality and quantity of PHB production. With these results, the use of haloarchaea as cell factories to produce PHAs is reinforced, and light is shed on the global concern about replacing plastics with biodegradable polymers.

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“…Additionally, Streptomyces possess genes encoding for metallothioneins and other metal-binding proteins that sequester HM intracellularly, providing an additional layer of protection [42,43]. These bacteria also contribute to the detoxi cation of contaminated soils by transforming HM into less toxic states through enzymatic processes [44,45].…”

Section: Discussionmentioning

confidence: 99%

Unraveling the role of contaminants reshaping the microflora in Zea mays seeds from heavy metal-contaminated and pristine environment

Awais,

Xianga,

Shah

et al. 2024

Preprint

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Heavy metal (HM) contaminants are the emerging driving force for reshaping the microflora of plants by eradicating the non-tolerance and non-resistant microbes via their lethal effects. Seeds serve as a primary source of microbial transmission from one plant generation to the next however, being exposed to metal contamination reshapes their microflora through generations. In this study, the endophytic diversity was compared within Zea mays seeds between three distinct regions in Yunnan province China i.e., HM-contaminated sites (AK) less-contaminated (SD), and a non-contaminated Site (DL). High throughput sequencing technique were used to analyze 492,177 high-quality reads for bacteria and 1,001,229 optimized sequences for fungi. Reads of interest were grouped into operational taxonomic units (OTUs), assigned to microbial genomes and analyzed for taxonomic information, alpha and beta diversity, taxonomic composition, and differential analyses. A higher diversity was recorded in AK samples than in SD and DL. Microbial community structure analysis showed higher diversity and significant fluctuation in specific taxa abundance in the metal-polluted samples exhibiting higher response of microbial flora to HM. In AK samples Gordonia and Burkholderia-Caballeronia-Paraburkholderi, were HM-resistant while Pseudomonas and Streptomyces showed moderate tolerance to HM. Fungal taxa such as Fusarium, Saccharomycopsis, and Lecanicillium also showed metal tolerance. The results revealed the role of HM in reshaping the microflora and their response in maize seeds. While investigating the microbiome from different environments of maize seeds using sequencing technology, the study will help in studying the microbiome shifts under HM conditions aiding better management strategies for reclaiming contaminated soil and crop management.

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Extracellular polymeric substances of acidophilic microorganisms play a crucial role in heavy metal ions adsorption

Cited by 16 publications

References 56 publications

Bioleaching of rare-earth elements from phosphate rock using Acidithiobacillus ferrooxidans

Bioleaching of rare-earth elements from phosphate rock using Acidithiobacillus ferrooxidans

Characterization of Polyhydroxybutyrate, PHB, Synthesized by Newly Isolated Haloarchaea Halolamina spp.

Unraveling the role of contaminants reshaping the microflora in Zea mays seeds from heavy metal-contaminated and pristine environment

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