Extracellular perfusion of rat brain nuclei using microdialysis: a method for studying differential neurotransmitter release in response to neurotoxins

Reynolds, Norman C.; Lin, Wen; Cameron, Carey Meyer; Roerig, David L.

doi:10.1016/s1385-299x(99)00008-2

Cited by 7 publications

(4 citation statements)

References 20 publications

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“… OB : Guevara -Guzman et al Guevara-Guzman et al ( 2000 ) PFC : Ballini et al ( 2008 ); Del Arco and Mora ( 1999 ); Del Arco and Mora ( 2000 ); Del Arco and Mora ( 2002 ); Grobin and Deutch ( 1998 ); Harte and O'Connor ( 2004 ); Hernandez et al ( 2008 ); Huang et al( 2008 ); Ohoyama et al ( 2011 ); Petkova-Kirova et al ( 2008 ) Pistis et al ( 2002 ); Welty and Shoblock ( 2009 ); Yamamura et al ( 2009a ) NAc: Dahchour ( 1996 ); Ferraro et al ( 1996a ); Hazell ( 1993 ); Hemmati et al ( 2001 ); Hernandez ( 2008 ); Huang et al ( 2008 ); Lindefors et al ( 1992 ); Reynolds et al ( 1999 ); Segovia ( 1999 ); Shou et al ( 2004 ); Smith and Sharp ( 1994 ); Tanganelli et al ( 1994 ); Xi et al ( 2003a ) CPu : Anderson and DiMicco ( 1992 ); Bourdelais and Deutch ( 1994 ); Del Arco et al ( 1998 ); Fantin et al ( 2007 ); Ferraro et al ( 1998 ); Ferraro et al ( 1997b ); Hernandez et al ( 2003 ); Hernandez et al ( 2008 ); Hondo et al ( 1995 ); Lillrank et al ( 1994 ); Meeusen et al ( 1997 ); Melani et al ( 2003 ); Molchanova et al ( 2004a ); Morari et al ( 1996 ); Morari et al ( 1993 ); Morari et al ( 1994 ); Segovia et al ( 1997 ); Segovia et al ( 1999 ); Semba et al ( 1995 ); Takeda et al ( 2003 ); Wang et al ( 2007 ); Yamamoto et al ( ...…”

Section: Resultsmentioning

confidence: 99%

Ethanol-induced alterations of amino acids measured by in vivo microdialysis in rats: a meta-analysis

Fliegel

Brand

Spanagel

et al. 2013

In Silico Pharmacol.

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PurposeIn recent years in vivo microdialysis has become an important method in research studies investigating the alterations of neurotransmitters in the extracellular fluid of the brain. Based on the major involvement of glutamate and γ-aminobutyric acid (GABA) in mediating a variety of alcohol effects in the mammalian brain, numerous microdialysis studies have focused on the dynamical behavior of these systems in response to alcohol.MethodsHere we performed multiple meta-analyses on published datasets from the rat brain: (i) we studied basal extracellular concentrations of glutamate and GABA in brain regions that belong to a neurocircuitry involved in neuropsychiatric diseases, especially in alcoholism (Noori et al., Addict Biol 17:827-864, 2012); (ii) we examined the effect of acute ethanol administration on glutamate and GABA levels within this network and (iii) we studied alcohol withdrawal-induced alterations in glutamate and GABA levels within this neurocircuitry.ResultsFor extraction of basal concentrations of these neurotransmitters, datasets of 6932 rats were analyzed and the absolute basal glutamate and GABA levels were estimated for 18 different brain sites. In response to different doses of acute ethanol administration, datasets of 529 rats were analyzed and a non-linear dose response (glutamate and GABA release) relationship was observed in several brain sites. Specifically, glutamate in the nucleus accumbens shows a decreasing logarithmic dose response curve. Finally, regression analysis of 11 published reports employing brain microdialysis experiments in 104 alcohol-dependent rats reveals very consistent augmented extracellular glutamate and GABA levels in various brain sites that correlate with the intensity of the withdrawal response were identified.ConclusionsIn summary, our results provide standardized basal values for future experimental and in silico studies on neurotransmitter release in the rat brain and may be helpful to understand the effect of ethanol on neurotransmitter release. Furthermore, this study illustrates the benefit of meta-analyses using the generalization of a wide range of preclinical data.

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Section: Resultsmentioning

confidence: 99%

Ethanol-induced alterations of amino acids measured by in vivo microdialysis in rats: a meta-analysis

Fliegel

Brand

Spanagel

et al. 2013

In Silico Pharmacol.

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“…This allows for GABA detection in the proximity of GABA sources (e.g., synapses). In contrast, microdialysis probes have a diameter of more than a millimeter (Reynolds et al, 1999) and are not adapted for local detection of transmitters.…”

Section: Discussionmentioning

confidence: 99%

“…During our experiments, we observed transient events with slow rise times (>10 ms) compared to fast IPSCs (<1 ms) measured in post-synaptic neurons (Korshoej et al, 2010; Xie and Manis, 2013). This observation can be interpreted in two ways: (1) the source of GABA was distant from the sniffer; (2) The release of GABA occurs through a slow process, such as diffusion through cation channels (Song et al, 2011) or via the reversal of a GABA transporter (Reynolds et al, 1999). It is unlikely that the intrinsic properties of the sniffer contributed to shaping of transient events because it could react to high concentrations of GABA with much faster kinetics (~100 pA/ms) than the events detected in the tissue (~10 pA/ms).…”

Section: Discussionmentioning

confidence: 99%

Fast detection of extrasynaptic GABA with a whole-cell sniffer

Christensen

Petersen

Schmitt

et al. 2014

Front. Cell. Neurosci.

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Gamma-amino-butyric acid (GABA) is the main inhibitory transmitter of the brain. It operates by binding to specific receptors located both inside and outside synapses. The extrasynaptic receptors are activated by spillover from GABAergic synapses and by ambient GABA in the extracellular space. Ambient GABA is essential for adjusting the excitability of neurons. However, due to the lack of suitable methods, little is known about its dynamics. Here we describe a new technique that allows detection of GABA transients and measurement of the steady state GABA concentration with high spatial and temporal resolution. We used a human embryonic kidney (HEK) cell line that stably expresses GABAA receptors composed of α1, β2, and γ2 subunits. We recorded from such a HEK cell with the whole-cell patch-clamp technique. The presence of GABA near the HEK cell generated a measurable electric current whose magnitude increased with concentration. A fraction of the current did not inactivate during prolonged exposition to GABA. This technique, which we refer to as a “sniffer” allows the measurement of ambient GABA concentration inside nervous tissue with a resolution of few tens of nanomolars. In addition, the sniffer detects variations in the extrasynaptic GABA concentration with millisecond time resolution. Pilot experiments demonstrate that the sniffer is able to report spillover of GABA induced by synaptic activation in real time. This is the first report on a GABA sensor that combines the ability to detect fast transients and to measure steady concentrations.

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“…3-Nitropropionic acid-intoxicated rats frequently serve as the animal model for HD (53,57). This acid can be characterized as excitotoxic compound and impaired mitochondrial energy component which produced lesions in the striatum, hippocampus, and corpus callosum but not in the cortex (7,9,19).…”

Section: Neurotoxicitymentioning

confidence: 99%