Extracellular microRNAs profile in human follicular fluid and IVF outcomes

Abstract

Encapsulated microRNAs (i.e., miRNAs within the extracellular vesicles, i.e., EV-miRNAs) have been detected in follicular fluid in both animal and human studies and different profiles have been associated with IVF cycle characteristics. However, limited studies to date have investigated other IVF outcomes, including fertilization status and embryo quality on day three”. In this cohort, we performed a cross-sectional analysis on 126 women who contributed follicular fluid from a single follicle during a single IVF cycle. One hundred and ninety-two EV-miRNAs were assessed by univariable fold-change and multivariable logistic regression analyses. Hsa-miR-92a and hsa-miR-130b, were over-expressed in follicular fluid samples from oocytes that failed to fertilize compared to those that were normally fertilized. Additionally, hsa-miR-888 was over-expressed and hsa-miR-214 and hsa-miR-454 were under-expressed in samples that resulted in impaired day-3 embryo quality compared to top-quality day-3 embryos. After adjusting for confounders as BMI, smoking and total motile sperm, associations of these EV-miRNAs remained significant. In-silico KEGG pathway analyses assigned the identified EV-miRNAs to pathways of follicular growth and development, cellular signaling, oocyte meiosis, and ovarian function. Our findings suggest that EV-miRNAs may play a role in pathways of ovarian function and follicle development, which could be essential for understanding the molecular mechanisms that could lead to a successful pregnancy and birth.

MicroRNA profiling in spermatozoa of men with unexplained asthenozoospermia

“…Our result was in contradiction to studies on semen (Tian et al, ) and seminal plasma (Qing et al, ) samples of AZS men that showed miR‐888‐5p downregulated in AZS samples in comparison with controls. Similar to our finding, it was shown that miR‐888‐5p overexpressed in samples of impaired embryo quality compared to top‐quality embryos (Martinez et al, ) and AZS spermatozoa (Abu‐Halima et al, ). The study on 69 unexplained AZS showed dysregulation of five miRNAs including miR‐888 that specifically expressed in the epididymis (Qing et al, ).…”

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

“…In addition, several evidences have shown predicted and experimentally validated l roles of extracellular vesicles-mediated miRNAs in female gametogenesis. For instance, higher expressions of exosome-derived miR-92a and miR-130b have been detected in follicular fluids of women with oocytes that failed to fertilize [78]. This indicate that miRNAs carried by extracellular-vesicles of the follicular fluid as additional molecules that control stage specific follicular development and oocyte maturation along with the autocrine and paracrine communication occurring in the follicular environment.…”

MiR‐27 alleviates myocardial cell damage induced by hypoxia/reoxygenation via targeting TGFBR1 and inhibiting NF‐κB pathway

“…miRNAs can regulate degradation or inhibit translation of messenger RNA (mRNA) by targeting their 3′ untranslated region (UTR), thereby regulating gene expression at the post‐transcriptional level . miRNA plays a key role in regulating tissue homeostasis and can contribute to various diseases . Various miRNAs were shown to control vascular inflammation, angiogenesis, and atherosclerosis in vascular system .…”

“…6 miRNA plays a key role in regulating tissue homeostasis and can contribute to various diseases. 7,8 Various miRNAs were shown to control vascular inflammation, angiogenesis, and atherosclerosis in vascular system. 9 It was found that the inhibition of miR-1 may have protective effects on myocardial I/R injury.…”