Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells

Ida, Takako; Kaku, Masaru; Kitami, Megumi; Terajima, Masahiko; Rocabado, Juan Marcelo Rosales; Akiba, Yosuke; Nagasawa, Masako; Yamauchi, Mitsuo; Uoshima, Katsumi

doi:10.1371/journal.pone.0204306

Cited by 32 publications

(25 citation statements)

References 52 publications

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“…The reflected colors from green, yellow, orange, to red correspond to the increase in thickness and maturation of collagen fibers, respectively , from which the organization and orientation of collagen fibers can be visualized . The use of digital image‐analysis software together with Picrosirius red‐polarization has been increasingly utilized for quantification of specific collagen fiber features in normal and pathological tissues or in cell/matrix layers generated in vitro . For measurement of total collagen content in tissues or matrices, quantification from Picrosirius red staining is still no match to the gold standard method of hydroxyproline analysis .…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Sinpreechanon

Boonzong

Sricholpech

2019

European J Oral Sciences

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Milk remains one of the most frequently recommended solutions for storage of avulsed teeth because it can maintain cell viability and is easily accessible. However, some negative effects of milk on avulsed teeth have been reported, just as the effects of milk on the long‐term functions of cells are not clear. This study aimed to evaluate the effects of different types of milk on the viability, proliferation, and functions of periodontal ligament fibroblasts (PDLF)s in vitro. Human PDLFs were culture‐medium depleted for 5 min and stored in Hanks’ balanced salt solution (HBSS), whole cow's milk, low‐fat cow's milk, or almond milk for 1 h at 25°C. Cell viability and proliferation were assessed using MTT assays. Expression of the genes encoding type I collagen and its modifying enzymes were analyzed using real‐time PCR. Collagen matrix production was evaluated using Picrosirius red polarization. Our results showed the overall efficiency of low‐fat cow's milk in maintaining the viability and proliferation of PDLFs, and in enhancing the process of collagen production. Almond milk storage resulted in the highest rate of PDLF proliferation, and comparable collagen biosynthesis ability to the control. Therefore, besides low‐fat cow's milk, almond milk may potentially be an alternative tooth‐storage medium for PDLF preservation and PDL tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Sinpreechanon

Boonzong

Sricholpech

2019

European J Oral Sciences

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“…Second, the highest fusion rate did not occur in the matrix that induces the highest CD47 gene expression. Both CD47 and DC-STAMP are considered to correlate positively with macrophage fusion 39 www.nature.com/scientificreports www.nature.com/scientificreports/ as decreasing the matrix stiffness by lysyl oxidase (LOX) inhibitor (β-aminopropionitrile) 40 . The expression of CD47 on stiff substrate does not correlate to macrophage fusion, but Sosale et al demonstrated that macrophages increased phagocytosis when sensed substrate stiffness via the expression of CD47 41 .…”

Section: Discussionmentioning

confidence: 99%

Switch of macrophage fusion competency by 3D matrices

Fang

Yang

Han

2020

Sci Rep

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Foreign body reaction reflects the integration between biomaterials and host cells. At the implantation microenvironment, macrophages usually fuse into multinuclear cells, also known as foreign body giant cells, to respond to the biomaterial implants. To understand the biomaterial-induced macrophage fusion, we examined whether biomaterial alone can initiate and control the fusion rate without exogenous cytokines and chemicals. We introduced a collagen-based 3D matrix to embed Raw264.7 cell line and primary rat bone marrow-derived macrophages. We found the biomaterial-stimuli interacted regional macrophages and altered the overall fusogenic protein expressions to regulate the macrophage fusion rate. The fusion rate could be altered by modulating the cell-matrix and cell-cell adhesions. The fused macrophage morphologies, the nuclei number in the fused macrophage, and the fusion rates were matrix dependent. The phenomena were also observed in the in vivo models. These results suggest that the biomaterial-derived stimuli exert similar functions as cytokines to alter the competency of macrophage fusion as well as their drug sensitivity in the biomaterial implanted tissue environment. Furthermore, this in vitro 3D-matrix model has the potential to serve as a toolbox to predict the host tissue response on implanted biomaterials.

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“…5c and e). The extent of collagen cross-linking of bone matrix affects the differentiation of bone cells (Ida et al, 2018). Therefore, we speculated that the cross-linking of collagen fibers could suppress bone remodeling and growth.…”

Section: Discussionmentioning

confidence: 99%

Electromyostimulation influences the mechanical properties and microarchitectures of bones beyond the stimulation site

Limbong

YAMAMURA

Tanaka

2019

JBSE

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Electromyostimulation is a nonpharmacological prevention method for osteoporosis that is safe and feasible for the elderly and people with physical disabilities. Our previous study demonstrated that random pulse train (RdPT) electromyostimulation of rat quadriceps induces an increase in the mechanical properties of the contralateral unstimulated femoral neck. However, the efficacy of this stimulation on other untested bones is still unclear. The objective of this research is to investigate the response of previously unstimulated bones to single-site electromyostimulation. The left quadriceps of rats were stimulated electrically by periodic pulse train (PrPT) or RdPT with 2 mA-magnitude pulses at 552 μs and a 50% duty ratio. The stimulation effect was examined on the diaphysis of long bones and lumbar vertebrae (L2-L5) by quasi-static mechanical tests and microcomputed tomography analysis. RdPT increased the strain energy at the stimulated left femur but did not change the properties of the other long bones. For the lumber vertebrae, on the other hand, both stimulations showed similar results. The stiffness of lumbar vertebra increased in L2, and the stiffness and the maximum load decreased in L4. Additionally, the BMC (bone mineral content), BV (bone volume), and TV (tissue volume) were reduced in L2, but not changed in L4. The other vertebrae were not affected by the stimulations. In conclusion, RdPT influences not only the stimulated femur, but also the lumbar vertebrae site-dependently as well as PrPT. These findings suggest the whole-body scale effect of electromyostimulation, however, which is not positive in all the bones, requiring further investigations for its clinical applications.

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Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells

Cited by 32 publications

References 52 publications

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Switch of macrophage fusion competency by 3D matrices

Electromyostimulation influences the mechanical properties and microarchitectures of bones beyond the stimulation site

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