2022
DOI: 10.1016/j.bbagen.2022.130238
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Extracellular matrix stiffness regulates degradation of MST2 via SCF βTrCP

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Cited by 6 publications
(10 citation statements)
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“…Our present study is consistent with previous studies that E3 Ub ligases, carboxy terminus of Hsp70-interacting protein and tumor necrosis factor receptor–associated factor 6, negatively regulate Mst1 stability, and that SCF βTrCP negatively regulates Mst2 stability through ubiquitination degradation in mammalian cells ( 28 , 29 , 30 ). Our finding further reveals the critical roles of Smurf1 in the regulation of ubiquitination-dependent degradation of Mst1/2 and thus expands the list of E3 Ub ligases that control the stability of Mst1/2.…”
Section: Discussionsupporting
confidence: 93%
“…Our present study is consistent with previous studies that E3 Ub ligases, carboxy terminus of Hsp70-interacting protein and tumor necrosis factor receptor–associated factor 6, negatively regulate Mst1 stability, and that SCF βTrCP negatively regulates Mst2 stability through ubiquitination degradation in mammalian cells ( 28 , 29 , 30 ). Our finding further reveals the critical roles of Smurf1 in the regulation of ubiquitination-dependent degradation of Mst1/2 and thus expands the list of E3 Ub ligases that control the stability of Mst1/2.…”
Section: Discussionsupporting
confidence: 93%
“…Based on these results, and our observations that Par-1 becomes more tightly associated with the cell cortex under increased cortical tension ( Figure 6 ), we propose that cortical tension could regulate Kib levels at least in part by modulating the association of Par-1 with Kib signaling complexes. Interestingly, a recent study in human cells also suggested that tension generated at the ECM promotes degradation of an Hpo homologue, MST2, by modulating the physical interaction between MST2 and SCF Slimb/βTrCP complex ( Fiore et al. , 2022 ).…”
Section: Discussionmentioning
confidence: 99%
“…For the Flag immunoprecipitation as previously described 19 , brie y immunoprecipitations were carried out using FLAG-M2 coated agarose beads (Sigma-Aldrich, #F2426) for 2 hours at 4°C. The beads were then extensively washed in lysis buffer and elution was carried out with 50 µL of 3X FLAG peptide (Sigma-Aldrich, #F4799), the super-natant was separated from the beads by centrifugation, resuspended in the same volume of 2X Laemmli sample buffer, heated at 95•C for 5 min and kept at -20°C, until used.…”
Section: Methodsmentioning
confidence: 99%
“…Generation of MCF10A and U2OS sublines for inducible overexpression of MST2 FL or MST2 ∆7 . From a plasmid previously designed by our group 19 , we ampli ed the Flag2x _Strep2x fused to the MST2 constructs (Full length (FL) and MST2 with exon 7 missing (∆7) with Q5 High-Fidelity Polymerase (New England BioLabs, #M0492) using speci c primers (EcoRI_Flag_Fw and MST2_AgeI_Rv, listed in Table 1)…”
Section: Methodsmentioning
confidence: 99%
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