Extracellular Matrix Remodeling and Mechanical Stresses as Modulators of Adipose Tissue Metabolism and Inflammation

Lee, Kyongbum; Kuo, Catherine K.

doi:10.1007/8415_2013_172

Cited by 5 publications

(10 citation statements)

References 61 publications

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“…Interestingly, we found that preadipocytes treated with TNFα and TGFβ1 increased MFAP5 mRNA and protein expression and IL‐6 increased MFAP5 mRNA expression, suggesting that MFAP5 expression is modified by these inflammatory factors. It is known that certain inflammatory factors secreted by macrophages can induce excessive deposition of ECM components in preadipocytes which could lead to AT stiffening, development of fibrosis and finally AT dysfunction and metabolic stress . Our findings suggest that obesity‐related inflammation either directly or indirectly increases MFAP5 expression in AT through effects on ECM possibly also leading to increased levels of MFAP5 in the interstitial space in AT.…”

Section: Discussionmentioning

confidence: 64%

“…Earlier studies have shown that the expression of ECM proteins is higher in the SVF cells like preadipocytes, compared to mature adipocytes, and the expression of ECM molecules is increased in preadipocytes in response to inflammatory factors, leading to reduction in AT expandability, development of fibrosis, and finally AT dysfunction . Because weight reduction results in downregulation of MFAP5 with concomitant changes in low‐grade inflammation and insulin sensitivity, we examined whether obesity‐related inflammatory factors modulate MFAP5 expression and the role of MFAP5 in adipocyte metabolism and insulin action using a human SGBS adipocyte cell strain.…”

Section: Discussionmentioning

confidence: 99%

“…ECM maintains the structural integrity of adipocytes in AT and during adipogenesis remodeling of ECM is needed to accommodate AT growth . Excess of ECM components can constrain adipocyte expandability and induce tissue stiffening, leading to metabolic stress . Therefore, downregulated MFAP5 expression during preadipocyte differentiation suggests that MFAP5 could be involved in adipogenesis‐related ECM remodeling which enables adipocyte enlargement.…”

Section: Discussionmentioning

confidence: 99%

“…Development of obesity is associated with adipose tissue (AT) remodeling, involving processes like adipogenesis and extracellular matrix (ECM) remodeling which are dependent on cellular events involving mature adipocytes and different cell types of the stromal vascular fraction (SVF) including preadipocytes, endothelial cells, fibroblasts, and different types of immune cells . In addition, SVF is a major source of ECM protein synthesis . Inflammatory factors secreted by macrophages have been shown to stimulate the excessive synthesis of ECM components (e.g., collagens) in preadipocytes .…”

Section: Introductionmentioning

confidence: 99%

“…Inflammatory factors secreted by macrophages have been shown to stimulate the excessive synthesis of ECM components (e.g., collagens) in preadipocytes . This contributes to a reduced plasticity of AT, leading to limitations in AT enlargement, and finally development of fibrosis that is suggested to be a link between obesity, diabetes, and cardiovascular disease .…”

Section: Introductionmentioning

confidence: 99%

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MFAP5is related to obesity-associated adipose tissue and extracellular matrix remodeling and inflammation

Vaittinen

Kolehmainen

Rydén

et al. 2015

Obesity

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Objective: Low-grade inflammation is involved in adipose tissue (AT) and extracellular matrix (ECM) remodeling and induces deposition of ECM proteins in AT. We have previously shown that MFAP5 (microfibrillar-associated protein 5) expression decreases in AT after weight loss. The aim of this study was to investigate MFAP5 localization in human AT and gene expression in adipocytes and the role of MFAP5 in adipocyte metabolism and inflammation. Methods: MFAP5 protein localization and gene expression were studied with immunohistochemistry and quantitative reverse transcriptase PCR (RT-qPCR) in human subcutaneous AT and cultured SimpsonGolabi-Behmel syndrome (SGBS) adipocytes, respectively. The effect of MFAP5 knock-down by siRNA on gene expression and insulin action was examined with RT-qPCR, western blot, and insulin-stimulated glucose uptake. The effect of different cytokines on MFAP5 gene and protein expression was investigated in cultured human SGBS preadipocytes. Results: MFAP5 protein was highly expressed in AT, and gene expression decreased during adipocyte differentiation in SGBS cells. Treatment of preadipocytes with TNFa and TGFb1 increased MFAP5 gene and protein expression. Furthermore, MFAP5 knock-down decreased the expression of genes involved in inflammation.Conclusions: Our results demonstrate that factors involving low-grade inflammation modulate MFAP5 expression and that the modified expression of MFAP5 may further regulate AT inflammation.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MFAP5is related to obesity-associated adipose tissue and extracellular matrix remodeling and inflammation

Vaittinen

Kolehmainen

Rydén

et al. 2015

Obesity

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Human Adipose Tissue Derivatives as a Potent Native Biomaterial for Tissue Regenerative Therapies

Sharath

Janarthanan

Nair

et al. 2020

Tissue Eng Regen Med

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BACKGROUND: Human adipose tissue is a great source of translatable biomaterials owing to its ease of availability and simple processing. Reusing discardable adipose tissue for tissue regeneration helps in mimicking the exact native microenvironment of tissue. Over the past 10 years, extraction, processing, tuning and fabrication of adipose tissue have grabbed the attention owing to their native therapeutic and regenerative potential. The present work gives the overview of next generation biomaterials derived from human adipose tissue and their development with clinical relevance. METHODS: Around 300 articles have been reviewed to widen the knowledge on the isolation, characterization techniques and medical applications of human adipose tissue and its derivatives from bench to bedside. The prospective applications of adipose tissue derivatives like autologous fat graft, stromal vascular fraction, stem cells, preadipocyte, adipokines and extracellular matrix, their behavioural mechanism, rational property of providing native bioenvironment, circumventing their translational abilities, recent advances in featuring them clinically have been reviewed extensively to reveal the dormant side of human adipose tissue. RESULTS: Basic understanding about the molecular and structural aspect of human adipose tissue is necessary to employ it constructively. This review has nailed the productive usage of human adipose tissue, in a stepwise manner from exploring the methods of extracting derivatives, concerns during processing and its formulations to turning them into functional biomaterials. Their performance as functional biomaterials for skin regeneration, wound healing, soft tissue defects, stem cell and other regenerative therapies under in vitro and in vivo conditions emphasizes the translational efficiency of adipose tissue derivatives. CONCLUSION: In the recent years, research interest has inclination towards constructive tissue engineering and regenerative therapies. Unravelling the maximum utilization of human adipose tissue derivatives paves a way for improving existing tissue regeneration and cellular based therapies and other biomedical applications.

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Towards an understanding of the mechanoreciprocity process in adipocytes and its perturbation with aging

Luca

Mandalà

Rose

2021

Mechanisms of Ageing and Development

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Extracellular Matrix Remodeling and Mechanical Stresses as Modulators of Adipose Tissue Metabolism and Inflammation

Cited by 5 publications

References 61 publications

MFAP5is related to obesity-associated adipose tissue and extracellular matrix remodeling and inflammation

MFAP5is related to obesity-associated adipose tissue and extracellular matrix remodeling and inflammation

Human Adipose Tissue Derivatives as a Potent Native Biomaterial for Tissue Regenerative Therapies

Towards an understanding of the mechanoreciprocity process in adipocytes and its perturbation with aging

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