Extracellular matrix proteins in the gastrointestinal tract: more than a supporting role

Browning, Kirsteen N.

doi:10.1113/jp276661

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…The use of functionalization methods was significantly associated to an increase of proteoglycans, and F-FAOM cultured for 3 weeks was only marginally different to control human oral mucosa biopsies. In addition, ECM glycoproteins are essential components of functional tissues and perform critical architectural and structural roles in native tissues [30]. The fact that n-FAOM tissues were virtually devoid of these ECM components is in agreement with our previous findings, suggesting that bioartificial tissues kept in vitro are not able to synthetize large amounts of ECM components in this stage of development [20].…”

Section: Discussionsupporting

confidence: 91%

In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

et al. 2020

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Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.

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Section: Discussionsupporting

confidence: 91%

In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

et al. 2020

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“…Several families of proteins with diverse origins and functions constitute the noncollagenous glycoproteins of the ECM. Among them, adhesive glycoproteins are a class of ECM macromolecules that exist in several variant forms, with multiple binding domains able to interact with collagen and proteoglycans, and to bind cell surfaces. , Representative adhesive glycoproteins are fibronectin (FN), laminin (LM), vitronectin (VN), thrombospondins (TSPs) and tenascin (TN). Most of the ECM glycoproteins interact with integrins and other receptors expressed at the cell surface, and with collagen or other components of the ECM .…”

Section: Ecm and Cell Microenvironment: Taking Inspiration From Naturementioning

confidence: 99%

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

et al. 2020

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Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tuning the biochemical and structural actors in synthetic ECMs. This review will present the fundamentals of the ECM, cover the chemical and structural features of the scaffolds used to generate ECM mimics, discuss the nature of the signaling biomolecules required and exploited to generate bioresponsive cell microenvironments able to induce a specific cell fate, and highlight the synthetic strategies involved in creating functional 3D ECM mimics.

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Biomaterials in Organoid Development

Samat

Yahaya

2022

Organoid Technology for Disease Modelling and Personalized Treatment

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Extracellular matrix proteins in the gastrointestinal tract: more than a supporting role

Cited by 3 publications

References 5 publications

In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

Biomaterials in Organoid Development

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