Extracellular matrix proteins in construction and function of in vitro blood-brain barrier models

Abstract

The blood-brain barrier (BBB) is a highly impermeable barrier separating circulating blood and brain tissue. A functional BBB is critical for brain health, and BBB dysfunction has been linked to the pathophysiology of diseases such as stroke and Alzheimer’s disease. A variety of models have been developed to study the formation and maintenance of the BBB, ranging from in vivo animal models to in vitro models consisting of primary cells or cells differentiated from human pluripotent stem cells (hPSCs). These models must consider the composition and source of the cellular components of the neurovascular unit (NVU), including brain microvascular endothelial cells (BMECs), brain pericytes, astrocytes, and neurons, and how these cell types interact. In addition, the non-cellular components of the BBB microenvironment, such as the brain vascular basement membrane (BM) that is in direct contact with the NVU, also play key roles in BBB function. Here, we review how extracellular matrix (ECM) proteins in the brain vascular BM affect the BBB, with a particular focus on studies using hPSC-derived in vitro BBB models, and discuss how future studies are needed to advance our understanding of how the ECM affects BBB models to improve model performance and expand our knowledge on the formation and maintenance of the BBB.

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“…The permeability of these barriers varies, but the BBB is the broadest and most constrained barrier ( 4 ). Comprising continuous capillary endothelial cells, a basement membrane, and a discontinuous stratum of pericytes and astrocytes, the BBB serves as a vital physical barrier ( 5 ). It safeguards the brain against potentially harmful substances in the bloodstream by inhibiting their accumulation and transportation into the brain ( 6 ).…”