Extracellular Matrix Proteins and Transcription of Matrix-Associated Genes in Mesenchymal Stromal Cells during Modeling of the Effects of Microgravity

Zhivodernikov, I. V.; Ratushnyy, A. Yu.; Матвеева, Д. К.; Буравкова, Л. Б.

doi:10.1007/s10517-020-05040-z

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…In addition, human mesenchymal stem cells exposed to 10 days s-µ g showed a decrease in collagen production, as well as a reduced expression of TIMP1 , TIMP3, and MMP11 genes, together with an elevated expression of tenascin and laminin subunit ( Zhivodernikov et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, s- and r-µ g influenced ECM proteins in a time-dependent manner ( Infanger et al, 2006 ) and cell-type dependent, resulting in increases or decreases of ECM components ( Infanger et al, 2006 ; Marrero et al, 2009 ; Zhivodernikov et al, 2020 ). In addition, PC MCS engineered on the RPM revealed a decrease in COL1A1 after 3 days and an increase after 5 days, whereas basement components like COL4A5 and LAMA3 as well as the cell adhesion molecule FN1 were elevated in MCS at both time points ( Hybel et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity

Dietrichs

Grimm

Sahana

et al. 2022

Front. Cell Dev. Biol.

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Prostate cancer metastasis has an enormous impact on the mortality of cancer patients. Factors involved in cancer progression and metastasis are known to be key players in microgravity (µg)-driven three-dimensional (3D) cancer spheroid formation. We investigated PC-3 prostate cancer cells for 30 min, 2, 4 and 24 h on the random positioning machine (RPM), a device simulating µg on Earth. After a 24 h RPM-exposure, the cells could be divided into two groups: one grew as 3D multicellular spheroids (MCS), the other one as adherent monolayer (AD). No signs of apoptosis were visible. Among others, we focused on cytokines involved in the events of metastasis and MCS formation. After 24 h of exposure, in the MCS group we measured an increase in ACTB, MSN, COL1A1, LAMA3, FN1, TIMP1, FLT1, EGFR1, IL1A, IL6, CXCL8, and HIF1A mRNA expression, and in the AD group an elevation of LAMA3, COL1A1, FN1, MMP9, VEGFA, IL6, and CXCL8 mRNAs compared to samples subjected to 1 g conditions. Significant downregulations in AD cells were detected in the mRNA levels of TUBB, KRT8, IL1B, IL7, PIK3CB, AKT1 and MTOR after 24 h. The release of collagen-1α1 and fibronectin protein in the supernatant was decreased, whereas the secretion of IL-6 was elevated in 24 h RPM samples. The secretion of IL-1α, IL-1β, IL-7, IL-2, IL-8, IL-17, TNF-α, laminin, MMP-2, TIMP-1, osteopontin and EGF was not significantly altered after 24 h compared to 1 g conditions. The release of soluble factors was significantly reduced after 2 h (IL-1α, IL-2, IL-7, IL-8, IL-17, TNF-α, collagen-1α1, MMP-2, osteopontin) and elevated after 4 h (IL-1β, IL-2, IL-6, IL-7, IL-8, TNF-α, laminin) in RPM samples. Taken together, simulated µg induced 3D growth of PC-3 cancer cells combined with a differential expression of the cytokines IL-1α, IL-1β, IL-6 and IL-8, supporting their involvement in growth and progression of prostate cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity

Dietrichs

Grimm

Sahana

et al. 2022

Front. Cell Dev. Biol.

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“…An upregulation of genes encoding core matrisome proteins was demonstrated in a number of papers [ 3 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 ]. In juvenile human fibroblasts, 3 days SMG (RPM) induced an increase of transcription of core basement membrane collagen IV, and transcription/translation of the principal core glycoprotein fibronectin, as well as of MMPs involved in ECM remodeling [ 3 ].…”

Section: Connective Tissue Matrisome and Microgravitymentioning

confidence: 99%

“…Upregulation of several genes encoding core proteins and glycoproteins in human adipose MSCs was described after 4 days of RPM exposure [ 74 ]. Though RPM exposure till day 10 affected fewer differentially expressed matrisome genes, a part of core matrisome encoded genes were still significantly upregulated, while genes encoding ECM-degrading enzyme and its inhibitors were downregulated [ 75 ].…”

Section: Connective Tissue Matrisome and Microgravitymentioning

confidence: 99%

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Microgravity Effects on the Matrisome

Буравкова

Ларина

Андреева

et al. 2021

Cells

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Gravity is fundamental factor determining all processes of development and vital activity on Earth. During evolution, a complex mechanism of response to gravity alterations was formed in multicellular organisms. It includes the “gravisensors” in extracellular and intracellular spaces. Inside the cells, the cytoskeleton molecules are the principal gravity-sensitive structures, and outside the cells these are extracellular matrix (ECM) components. The cooperation between the intracellular and extracellular compartments is implemented through specialized protein structures, integrins. The gravity-sensitive complex is a kind of molecular hub that coordinates the functions of various tissues and organs in the gravitational environment. The functioning of this system is of particular importance under extremal conditions, such as spaceflight microgravity. This review covers the current understanding of ECM and associated molecules as the matrisome, the features of the above components in connective tissues, and the role of the latter in the cell and tissue responses to the gravity alterations. Special attention is paid to contemporary methodological approaches to the matrisome composition analysis under real space flights and ground-based simulation of its effects on Earth.

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Effects of short-term simulated microgravity on changes in extracellular space structure and substance diffusion and clearance

Luo,

Yang,

Zhao

et al. 2024

Acta Astronautica

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Extracellular Matrix Proteins and Transcription of Matrix-Associated Genes in Mesenchymal Stromal Cells during Modeling of the Effects of Microgravity

Cited by 10 publications

References 14 publications

Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity

Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity

Microgravity Effects on the Matrisome

Effects of short-term simulated microgravity on changes in extracellular space structure and substance diffusion and clearance

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