Extracellular matrix protein 1 (ECM1) is over-expressed in malignant epithelial tumors

Wang, Luping; Yu, Jun; Ni, Jian; Xu, Xueming; Wang, Jianjin; Ning, Hao-yong; Pei, Xu-Fang; Chen, Jinguo; Yang, Shanmin; Underhill, Charles B.; Liu, Lei; Liekens, Joeri; Merregaert, J.; Zhang, Lurong

doi:10.1016/s0304-3835(03)00350-1

Cited by 97 publications

(91 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of ECM1 is closely associated with early stages of angiogenesis during embryonic development and this protein has also been shown to be expressed by tumor cells. 36,36,37 Concordant with our results, ECM1 expression is associated with metastatic behavior. 37 Several other class 3 semaphorins and plexins have been found to affect tumor growth and invasion.…”

Section: Plxnd1 and Sema3e In Tumor Progressionsupporting

confidence: 91%

“…36,36,37 Concordant with our results, ECM1 expression is associated with metastatic behavior. 37 Several other class 3 semaphorins and plexins have been found to affect tumor growth and invasion. Whereas Sema3A, 3B, and 3F are described to be functional inhibitors of tumorigenesis and involved in inhibition of tumor cell spread, 38 -40 binding of Sema4D to its high affinity receptor plexin B1 contributes to invasive tumor growth and metastasis via activation of Met and Ron receptors.…”

Section: Plxnd1 and Sema3e In Tumor Progressionsupporting

confidence: 91%

See 1 more Smart Citation

Semaphorin 3E Expression Correlates Inversely with Plexin D1 During Tumor Progression

Roodink

Kats

Kempen

et al. 2008

The American Journal of Pathology

View full text Add to dashboard Cite

Plexin D1 (PLXND1) is broadly expressed on tumor vessels and tumor cells in a number of different human tumor types. Little is known, however, about the potential functional contribution of PLXND1 expression to tumor development. Expression of semaphorin 3E (Sema3E), one of the ligands for PLXND1, has previously been correlated with invasive behavior and metastasis, suggesting that the PLXND1-Sema3E interaction may play a role in tumor progression. Here we investigated PLXND1 and Sema3E expression during tumor progression in cases of melanoma. PLXND1 was not expressed by melanocytic cells in either naevi or melanomas in situ, whereas expression increased with invasion level, according to Clark's criteria. Furthermore, 89% of the metastatic melanomas examined showed membranous PLXND1-staining of tumor cells. Surprisingly, expression of Sema3E was inversely correlated with tumor progression, with no detectable staining in melanoma metastasis. To functionally assess the effects of Sema3E expression on tumor development, we overexpressed Sema3E in a xenograft model of metastatic melanoma. Sema3E expression dramatically decreased metastatic potential. These results show that PLXND1 expression during tumor development is strongly correlated with both invasive behavior and metastasis, but exclude Sema3E as an activating ligand.

show abstract

Section: Plxnd1 and Sema3e In Tumor Progressionsupporting

confidence: 91%

Section: Plxnd1 and Sema3e In Tumor Progressionsupporting

confidence: 91%

Semaphorin 3E Expression Correlates Inversely with Plexin D1 During Tumor Progression

Roodink

Kats

Kempen

et al. 2008

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…NF-kappaB is the key immune regulator in the pathogenesis of IBD (28)(29)(30). It has been also shown that ECM1 is overexpressed in metastatic epithelial tumors, including gastric and colorectal cancers, and its expression in hepatocellular carcinoma (HCC) is correlated with the metastatic potential of HCC (9,31).…”

Section: Discussionmentioning

confidence: 99%

“…Mutations that lead to tissue loss in patients with ECM1 SNPs probably increase the tissue injury caused by MMP-9, resulting in an increase in intestinal ulcers and scars in IBD (8). In addition, ECM1 is also associated with cell proliferation, angiogenesis, and differentiation; ECM1 expression has been demonstrated to be increased in metastatic epithelial tumors, such as those in gastric and colorectal cancers (9). Moreover, rs3737240 and rs13294, which are the strongest ECM1 markers, are also found to be associated with ankylosing spondylitis (10).…”

mentioning

confidence: 99%

Extracellular matrix protein 1 gene rs3737240 single nucleotide polymorphism is associated with ulcerative colitis in Turkish patients

Adali

Tunalı²,

Yorulmaz³

et al. 2017

Turk J Gastroenterol

View full text Add to dashboard Cite

“…Furthermore, ECM1 expression correlated with the metastatic potential of tumors in one study. Primary breast cancers that had formed metastases were 76% positive while those that had not metastasized were only 33% positive for expression [26].…”

Section: Protein Identificationmentioning

confidence: 99%

Identification of metastasis‐associated proteins in a human tumor metastasis model using the mass‐mapping technique

et al. 2004

View full text Add to dashboard Cite

For most cancer cell types, the acquisition of metastatic ability leads to clinically incurable disease. The identification of molecules whose expression is specifically correlated with the metastatic spread of cancer would facilitate the design of therapeutic interventions to inhibit this lethal process. In order to facilitate metastasis gene discovery we have previously characterized a pair of monoclonal cell lines from the human breast carcinoma cell line MDA-MB-435 that have different metastatic phenotypes in immune-compromised mice. In this study, serum-free conditioned media was collected from the cultured monoclonal cell lines and a mass mapping technique was applied in order to profile a component of each cell line proteome. We utilized chromatofocusing in the first dimension to obtain a high resolution separation based on protein pI, and nonporous silica reverse-phase high performance liquid chromatography was used for the second dimension. Selected proteins were identified on the basis of electrospray ionization time of flight mass spectrometry (ESI-TOF MS) intact protein mapping and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) peptide mass fingerprinting. Using this approach we were able to map over 400 proteins and plot them as a 2-D map of pI versus accurate M r . This was performed over a pI range of 4.0-6.2, and a mass range of 6-80 kDa. ESI-TOF MS data and further analysis using MALDI-TOF MS confirmed and identified 27 differentially expressed proteins. Proteins associated with the metastatic phenotype included osteopontin and extracellular matrix protein 1, whereas the matrix metalloproteinase-1 and annexin 1 proteins were associated with the non-metastatic phenotype. These findings demonstrate that the mass mapping technique is a powerful tool for the detection and identification of proteins in complex biological samples and which are specifically associated with a cellular phenotype.

show abstract

Extracellular matrix protein 1 (ECM1) is over-expressed in malignant epithelial tumors

Cited by 97 publications

References 13 publications

Semaphorin 3E Expression Correlates Inversely with Plexin D1 During Tumor Progression

Semaphorin 3E Expression Correlates Inversely with Plexin D1 During Tumor Progression

Extracellular matrix protein 1 gene rs3737240 single nucleotide polymorphism is associated with ulcerative colitis in Turkish patients

Identification of metastasis‐associated proteins in a human tumor metastasis model using the mass‐mapping technique

Contact Info

Product

Resources

About