Extracellular matrix powder from cultured cartilage-like tissue as cell carrier for cartilage repair

Abstract

Efficiently cultivating cartilage decellularized matrix powder by a 3D culture method for cartilage repair in rabbit osteochondral defects.

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

“…Cell‐seeded dECM particles can be delivered into the defect site as a particle aggregate . Yin et al generated dECM particles from goat articular cartilage.…”

“…These dECM particles were then seeded with MSCs and aggregated in a rotary cell culture system . Teng et al cultured cartilage‐like tissue by culturing chondrocyte spheroids in suspension in vitro, which were subsequently decellularized and milled into particles . In both cases, dECM particles not only induced chondrogenic behavior from seeded MSCs in vitro, but the MSC/particle aggregate also promoted osteochondral defect repair in vivo.…”

“…Cell-seeded dECM particles can be delivered into the defect site as a particle aggregate. 123,124 Yin et al generated dECM particles from goat articular cartilage. These dECM particles were then seeded with MSCs and aggregated in a rotary cell culture system.…”

Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges

“…Given the complexity and difficulty in totally mimicking the highly sophisticated native ECM with comparable composition and architecture, decellularization of whole tissues or organs by removing all cells and genetic material while maintaining the physical and biochemical characteristics has emerged as a possible alternative method for cartilage and osteochondral repair [ [201] , [202] , [203] ]. The resulting decellularized extracellular matrix (dECM) can be further processed to serve as a whole scaffold [ [204] , [205] , [206] , [207] , [208] ], a solubilized hydrogel component [ 123 , [209] , [210] , [211] ], a bioink for additive manufacturing [ [212] , [213] , [214] , [215] , [216] ] or pulverized particles for composite scaffold fabrication [ 205 , [217] , [218] , [219] ]. Lin et al produced a biphasic decellularized osteochondral ECM scaffold by combining physical freezing-thawing methods and chemical treatments.…”

Stem Cells in Autologous Microfragmented Adipose Tissue: Current Perspectives in Osteoarthritis Disease

“…The chondrocytes are located within the extracellular matrix (ECM) and housed in the “lacunae” of the ECM or organized in small single-cell groups which derive from named “isogenic groups” ( Figure 1 A). ECM (or intercellular substance) is amorphous with abundant aggrecan, collagen fibers (types III, VI, IX, XI), proteoglycans (decorin, byglican and fibromodulin) and glycosaminoglycans but mainly lacking in elastic fibers [ 13 ]. AC are arranged in four layers ( Figure 1 B,C): 1.Tangential zone (superficial with disk-shaped chondrocytes and horizontally located collagen fibrils; 2.Transitional zone (middle): Collagen fibrils in the middle zone are diagonally oriented and round chondrocytes are scattered irregularly; 3.…”