Extracellular Matrix of the Skin: 50 Years of Progress.

Uitto, Jouni; Olsen, David R.; Fazio, Michael

doi:10.1111/1523-1747.ep13075039

Cited by 88 publications

(81 citation statements)

References 189 publications

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“…In addition, type III collagen, which is structurally similar to type I collagen, did not induce collagenase-1 expression (Table I). Together these findings suggest that cell contact with type I collagen, the most abundant protein in the dermis, comprising greater than 70% of its dry weight (19), provides a critical and specific determinant regulating collagenase-1 production by keratinocytes. Furthermore, our findings suggest that re-establishment of the basement membrane and cell:cell contacts at the completion of wound closure mediates down-regulation of collagenase-1 expression.…”

Section: Discussionmentioning

confidence: 81%

Induction and Repression of Collagenase-1 by Keratinocytes Is Controlled by Distinct Components of Different Extracellular Matrix Compartments

Sudbeck¹,

Pilcher²,

Welgus³

et al. 1997

Journal of Biological Chemistry

108

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In all forms of cutaneous wounds, collagenase-1 (matrix metalloproteinase-1 (MMP-1)) is invariably expressed by basal keratinocytes migrating over the dermal matrix. We report that native type I collagen mediates induction of MMP-1 by primary human keratinocytes. Collagen-mediated induction of MMP-1 was rapid, being detected 2 h after plating, and was transcriptionally regulated. As demonstrated by in situ hybridization, only migrating keratinocytes expressed MMP-1, suggesting that contact with collagen is not sufficient to induce MMP-1 expression in keratinocytes; the cells must also be migrating. Upon denaturation, type I collagen lost its ability to induce MMP-1 expression but still supported cell adhesion. Other dermal or wound matrix proteins, such as type III collagen, fibrin, and fibronectin, and a mixture of basement membrane proteins did not induce MMP-1 production. In the presence of collagen, laminin-1 inhibited induction of MMP-1 but laminin-5 did not. Taken together, these observations suggest that as basal keratinocytes migrate from the basal lamina onto the dermal matrix contact with native type I collagen induces MMP-1 expression. In addition, our findings suggest that re-establishment of the basement membrane and, in particular, contact with laminin-1 provides a potent signal to down-regulate MMP-1 production as the epithelium is repaired.Normal cutaneous wound healing, as well as healing in essentially all tissues, involves an orderly progression of events to re-establish the integrity of the injured tissue. The initial injury starts a programmed series of interdependent yet functionally separate responses, such as re-epithelialization and epithelial proliferation, inflammation, angiogenesis, fibroplasia, matrix accumulation, and eventually resolution. During each stage in this process, proteinases are needed to remove or remodel extracellular matrix components in both the epithelial and interstitial compartments to accommodate cell migration and tissue repair (1).In a thorough examination of normally healing wounds and of a variety of chronic ulcers, we found that collagenase-1, a member of the matrix metalloproteinase (MMP) 1 family with the ability to cleave fibrillar collagens type I, II, and III at a specific locus in their triple helical domain, is invariably expressed by basal keratinocytes at the edge of repairing tissue (2-4). In all, we have examined Ͼ100 different human skin specimens, representing a variety of chronic ulcers, blisters, and normally healing wounds, and in each sample with injury that breached the basement membrane, collagenase-1 was prominently and invariantly expressed by basal keratinocytes migrating over the dermal wound bed. The invariable expression of collagenase-1 in all forms of wounds and the confinement of its expression to periods of active re-epithelialization suggest that this enzyme plays a critical role in keratinocyte function during healing. Indeed, we recently demonstrated that keratinocyte migration on a native collagen matrix requires the catalyti...

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Section: Discussionmentioning

confidence: 81%

Induction and Repression of Collagenase-1 by Keratinocytes Is Controlled by Distinct Components of Different Extracellular Matrix Compartments

Sudbeck¹,

Pilcher²,

Welgus³

et al. 1997

Journal of Biological Chemistry

108

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“…For example, scarring and impaired wound healing are significant clinical challenges related to traumatic and surgical wounds, and co-morbidities of diseases like diabetes. The main matrix components in skin are collagens, elastin, GAGs, laminin, nidogen and fibronectin 78 , and matrices derived from fibroblasts, the primary cell type within the dermis, recapitulate some the main components in adult skin 10, 57, 79 . Acellular dermal matrix allografts and skin substitutes, such as INTEGRA® Dermal Regeneration Template, are routinely used in the clinic to treat burns and chronic wounds 80-83 .…”

Section: Embryonic Stem Cell-derived Matricesmentioning

confidence: 99%

Cell-derived matrices for tissue engineering and regenerative medicine applications

2015

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The development and application of decellularized extracellular matrices (ECM) has grown rapidly in the fields of cell biology, tissue engineering and regenerative medicine in recent years. Similar to decellularized tissues and whole organs, cell-derived matrices (CDMs) represent bioactive, biocompatible materials consisting of a complex assembly of fibrillar proteins, matrix macromolecules and associated growth factors that often recapitulate, at least to some extent, the composition and organization of native ECM microenvironments. The unique ability to engineer CDMs de novo based on cell source and culture methods makes them an attractive alternative to conventional allogeneic and xenogeneic tissue-derived matrices that are currently harvested from cadaveric sources, suffer from inherent heterogeneity, and have limited ability for customization. Although CDMs have been investigated for a number of biomedical applications, including adhesive cell culture substrates, synthetic scaffold coatings, and tissue engineered products, such as heart valves and vascular grafts, the state of the field is still at a relatively nascent stage of development. In this review, we provide an overview of the various applications of CDM and discuss successes to date, current limitations and future directions.

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“…The predominant extracellular matrix component of the dermis and a variety of other human tissues is collagen [25]. During the first days after skin wounding type III collagen appears first and it is followed by type I collagen [5,11].…”

Section: Discussionmentioning

confidence: 99%

Procollagen propeptides in chronic subdural hematoma reveal sustained dural collagen synthesis after head injury

2009

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Extracellular Matrix of the Skin: 50 Years of Progress.

Cited by 88 publications

References 189 publications

Induction and Repression of Collagenase-1 by Keratinocytes Is Controlled by Distinct Components of Different Extracellular Matrix Compartments

Induction and Repression of Collagenase-1 by Keratinocytes Is Controlled by Distinct Components of Different Extracellular Matrix Compartments

Cell-derived matrices for tissue engineering and regenerative medicine applications

Procollagen propeptides in chronic subdural hematoma reveal sustained dural collagen synthesis after head injury

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