Extracellular matrix mechanics regulate transfection and SOX9-directed differentiation of mesenchymal stem cells

Ledo, Adriana M.; Vining, Kyle H.; Alonso, Marı́a José; García-Fuentes, Marcos; Mooney, David J.

doi:10.1016/j.actbio.2020.04.027

Cited by 38 publications

(31 citation statements)

References 79 publications

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“…Incorporation with other materials can improve the mechanical properties. It is reported that collagen in the combination with alginate not only enhances the mechanical properties of the composite hydrogel but also tunes the stiffness of CAC hydrogel simply via the concentration of Ca 2+ [ 26 ]. It is worth noting that stiffness is of great importance in the determination of stem cell fate [ 75 ].…”

Section: General Properties Of Alginate and Collagenmentioning

confidence: 99%

“…Moreover, bMSCs were capable of differentiating into chondrocytes by inducible agents secreted by co-cultured chondrocytes in vitro [ 111 ]. In addition, Ledo et al (2020) successfully incorporated SOX9 polynucleotides, which play a vital role in chondrogenesis, into CAC hydrogel, which may provide new approach for cartilage tissue engineering [ 26 ].…”

Section: Application Of Cac Hydrogelmentioning

confidence: 99%

“…Cell-adhesion ligands also increase as collagen concentration increases. Reproduced from [ 26 ] with permission, Copyright (2020) Elsevier.…”

Section: Figurementioning

confidence: 99%

“…Therefore, CAC hydrogel is widely investigated for cell encapsulation and drug delivery [ 22 , 23 , 24 , 25 ]. What is more, the combination of collagen and alginate provides cell-binding motifs and enhances the mechanical properties [ 26 ]. There is a growing body of investigations into CAC hydrogel and its application in tissue engineering and biomedical sciences, including tissue regeneration, wound dressing, encapsulated cell therapy (ECT), and tumor biology.…”

Section: Introductionmentioning

confidence: 99%

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Collagen–Alginate Composite Hydrogel: Application in Tissue Engineering and Biomedical Sciences

2021

Polymers

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Alginate (ALG), a polysaccharide derived from brown seaweed, has been extensively investigated as a biomaterial not only in tissue engineering but also for numerous biomedical sciences owing to its wide availability, good compatibility, weak cytotoxicity, low cost, and ease of gelation. Nevertheless, alginate lacks cell-binding sites, limiting long-term cell survival and viability in 3D culture. Collagen (Col), a major component protein found in the extracellular matrix (ECM), exhibits excellent biocompatibility and weak immunogenicity. Furthermore, collagen contains cell-binding motifs, which facilitate cell attachment, interaction, and spreading, consequently maintaining cell viability and promoting cell proliferation. Recently, there has been a growing body of investigations into collagen-based hydrogel trying to overcome the poor mechanical properties of collagen. In particular, collagen–alginate composite (CAC) hydrogel has attracted much attention due to its excellent biocompatibility, gelling under mild conditions, low cytotoxicity, controllable mechanic properties, wider availability as well as ease of incorporation of other biomaterials and bioactive agents. This review aims to provide an overview of the properties of alginate and collagen. Moreover, the application of CAC hydrogel in tissue engineering and biomedical sciences is also discussed.

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Section: General Properties Of Alginate and Collagenmentioning

confidence: 99%

Section: Application Of Cac Hydrogelmentioning

confidence: 99%

“…Cell-adhesion ligands also increase as collagen concentration increases. Reproduced from [ 26 ] with permission, Copyright (2020) Elsevier.…”

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Collagen–Alginate Composite Hydrogel: Application in Tissue Engineering and Biomedical Sciences

2021

Polymers

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“…Mechanobiology studies have revealed that, amongst other cues, cells are also responsive to the material stiffness (Discher et al, 2005 ), which can affect intracellular signaling cascades that trigger cell adhesion, phenotype maintenance, cytoskeletal reconstruction, and even stem cell differentiation (Engler et al, 2006 ; Mao et al, 2016 ; Cao et al, 2017 ; Kumar et al, 2017 ; Vining and Mooney, 2017 ; Darnell et al, 2018 ). Therefore, selection of biomaterials with the appropriate stiffness is not only important for matching the native tissue mechanical properties, but can be also used as a tool to control cell phenotypes and thus modulate cell behavior (Khademhosseini and Langer, 2016 ; Ledo et al, 2020b ). Furthermore, materials can undergo several chemical modifications, to improve their physicochemical properties as well as to enable incorporation of biologically relevant molecules and signals necessary for guiding and regulating cell response.…”

Section: Building Blocks For Developing Human Tissue Equivalentsmentioning

confidence: 99%

Advances in Engineering Human Tissue Models

Moysidou

Barberio

Owens

2021

Front. Bioeng. Biotechnol.

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Research in cell biology greatly relies on cell-based in vitro assays and models that facilitate the investigation and understanding of specific biological events and processes under different conditions. The quality of such experimental models and particularly the level at which they represent cell behavior in the native tissue, is of critical importance for our understanding of cell interactions within tissues and organs. Conventionally, in vitro models are based on experimental manipulation of mammalian cells, grown as monolayers on flat, two-dimensional (2D) substrates. Despite the amazing progress and discoveries achieved with flat biology models, our ability to translate biological insights has been limited, since the 2D environment does not reflect the physiological behavior of cells in real tissues. Advances in 3D cell biology and engineering have led to the development of a new generation of cell culture formats that can better recapitulate the in vivo microenvironment, allowing us to examine cells and their interactions in a more biomimetic context. Modern biomedical research has at its disposal novel technological approaches that promote development of more sophisticated and robust tissue engineering in vitro models, including scaffold- or hydrogel-based formats, organotypic cultures, and organs-on-chips. Even though such systems are necessarily simplified to capture a particular range of physiology, their ability to model specific processes of human biology is greatly valued for their potential to close the gap between conventional animal studies and human (patho-) physiology. Here, we review recent advances in 3D biomimetic cultures, focusing on the technological bricks available to develop more physiologically relevant in vitro models of human tissues. By highlighting applications and examples of several physiological and disease models, we identify the limitations and challenges which the field needs to address in order to more effectively incorporate synthetic biomimetic culture platforms into biomedical research.

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Structural and Functional Adaptive Artificial Bone: Materials, Fabrications, and Properties

Feng

Zhao

Tang

et al. 2023

Adv Funct Materials

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It is an urgent need that defect repair can develop from simple device fixation to living tissue reconstruction, from short life function replacement to permanent regeneration repair. At present, bone transplantation has become the second largest transplantation surgery after blood transfusion, and artificial bone transplantation generates great hope for the repair and treatment of bone defect. In order to repair bone defect, artificial bone must have good biological properties and sufficient mechanical properties, and it should also have the shape matching to bone defect site and the connected porous structure. For structures and properties requirements of artificial bone, in this review three major challenges faced by artificial bone transplantation are systemtically analyzed and current methods and strategies to address these issues are discussed: 1) the need for developing a type of bone scaffold material with both biological and mechanical properties, 2) the need for realizing the controllable fabrication of individual shape and multistage pore structure of bone scaffold, 3) the need for realizing the transformation from man‐made structure to biological structure. Besides, it summarizes the advantages and disadvantages of these methods and discusses the potential future directions of structural and functional adaptive artificial bone for bone defect regeneration.

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Extracellular matrix mechanics regulate transfection and SOX9-directed differentiation of mesenchymal stem cells

Cited by 38 publications

References 79 publications

Collagen–Alginate Composite Hydrogel: Application in Tissue Engineering and Biomedical Sciences

Collagen–Alginate Composite Hydrogel: Application in Tissue Engineering and Biomedical Sciences

Advances in Engineering Human Tissue Models

Structural and Functional Adaptive Artificial Bone: Materials, Fabrications, and Properties

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