Extracellular matrix in epitheliochorial, endotheliochorial and haemochorial placentation and its potential application for regenerative medicine

Anunciação, Ara da; Mess, Andrea; Orechio, Dailiany; Aguiar, BA; Favaron, Phelipe Oliveira; Moody, M. Anthony

doi:10.1111/rda.12868

Cited by 18 publications

(12 citation statements)

References 180 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In all invasion assays performed Matrigel, the basement membrane product composed of laminin, fibronectin and proteoglycans, was used 29 . In contrast to previously published data 30 , dot blot and WB analysis detected galectin-3 and galectin-1 in all tested basement membrane preparations, as bands of ~30 kDa and 14.5 kDa, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

Human trophoblast requires galectin-3 for cell migration and invasion

Bojić‐Trbojević

Krivokuća

Vilotić

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Invasive extravillous cytotrophoblast of the human placenta expresses galectins-1, -3, and -8 in vivo and in vitro. This study aimed to investigate the potential role of galectin-3 in cell migration and invasion, using recombinant human galectin-3 (rhgalectin-3), small molecule galectin inhibitor I47, and galectin-3 silencing. HTR-8/SVneo cell migration was stimulated by rhgalectin-3 and reduced by I47, which could be neutralised by rhgalectin-3. Inhibitor specificity and selectivity for the galectins expressed in extravillous trophoblast were validated in solid phase assays using recombinant galectin-1, -3, -8, confirming selectivity for galectin-3. HTR-8/SVneo cell migration and invasion, and invasion by isolated trophoblast cells in primary culture were significantly reduced in the presence of I47, which could be restored by rhgalectin-3. Upon HTR-8/SVneo cell treatment with galectin-3 siRNA both LGALS3 and galectin-3 protein were dramatically decreased. Silencing of galectin-3 induced significant reduction in cell migration and invasion, which was restored by rhgalectin-3. The influence on known mediators of cell invasion, MMP2 and -9, and integrins α1, α5, and β1 was followed in silenced cells, showing lower levels of MMPs and a large reduction in integrin subunit β1. These results show that galectin-3 acts as a pro-invasive autocrine/paracrine factor in trophoblast in vitro.

show abstract

Section: Resultsmentioning

confidence: 99%

Human trophoblast requires galectin-3 for cell migration and invasion

Bojić‐Trbojević

Krivokuća

Vilotić

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In most respects, these adhesive processes may include an interplay between extracellular matrix (ECM) proteins. The ECM, which fills the intercellular space and provides an appropriate environment for animal cells and also regulates cell functions through signaling, proliferation, migration and invasion, consists of several simple proteins and proteoglycans [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

2018

View full text Add to dashboard Cite

Decorin is a small leucine-rich proteoglycan which is involved in multiple biological functions mainly as a structural and signaling molecule. Due to its biological properties in connective tissue, decorin may participate in remodeling of ECM during attachment and detachment of placenta within the course of pregnancy and at parturition in cows. The aim of the study was to detect the presence of decorin protein in bovine placental tissues and to evaluate its profile during pregnancy and at parturition. Placental tissues from healthy pregnant cows (2–5 month) were collected in abattoir (n = 10), while parturient tissues were obtained during caesarian section at physiological term (n = 6). Maternal and fetal parts were separated manually and subjected to homogenization and to quantitative ELISA and verification by Western blotting with anti-decorin antibodies. ELISA test showed that the concentration of decorin during pregnancy was higher in the fetal part of placenta compared with the maternal part (p < 0.001). Similar pattern was noted regarding to maternal and fetal samples derived from parturient cows. Our preliminary results demonstrate that the concentration of decorin is gestation time-dependent in healthy bovine placenta. Possible confirmation of the involvement of decorin in early pregnancy attachment and detachment of the placenta during parturition requires further research.

show abstract

“…During this period, the placenta plays a vital role in the maintenance of the embryos and fetuses (Wooding and Burton, 2008). The pig placenta is diffuse, rough and epitheliochorial,althoughimportant morphogenetic changes occur in this organ during gestation (Dantzer and Winther, 2001;da Anunciação et al, 2017;Croy et al, 2009) Placentation includes extensive angiogenesis in maternal and extraembryonic tissues, accompanied by a marked increase in uterine and umbilical blood flow (Goldstein et al, 1980;Biensen et al, 1998;Reynolds and Redmer, 2001;Merkis et al, 2006;Reynolds et al, 2006;Gourvas et al, 2012). The dense networks of blood vessels within the placenta are responsible for exchanging respiratory gases, nutrients and wastes between the mother and the fetus throughout pregnancy, which is essential for proper fetal growth (Mayhew, 2002;Burton et al, 2009;Sanchis et al, 2015;Pereira et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis and cell proliferation in porcine placental vascularization

Sanchis

Cristofolini

Fiorimanti

et al. 2017

Animal Reproduction Science

View full text Add to dashboard Cite

The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30±2(n=5), 40±2(n=5), 60±2 (n=5), 80±2(n=5), 90±2(n=5) and 114±2(n=5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30±2 and 80±2 of pregnancy. Caspase-3 expressed the highest value on day 30±2, while the highest apoptotic indexes were found on days30±2 and 90±2. The placental vascular area was higher on day 80±2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80±2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.

show abstract

Extracellular matrix in epitheliochorial, endotheliochorial and haemochorial placentation and its potential application for regenerative medicine

Cited by 18 publications

References 180 publications

Human trophoblast requires galectin-3 for cell migration and invasion

Human trophoblast requires galectin-3 for cell migration and invasion

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

Apoptosis and cell proliferation in porcine placental vascularization

Contact Info

Product

Resources

About