Extracellular Matrix Gene Expression by Human Bone Marrow Stroma and by Marrow Fibroblasts

Chichester, C. O.; Fernández, Mireya; Minguell, José J.

doi:10.3109/15419069309095685

Cited by 49 publications

(34 citation statements)

References 30 publications

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“…Together, their capacity to produce ECM proteins (this report and Chichester et al, 1993;Haynesworth et al, 1995) and cytokines (Haynesworth et al, 1996) confess to MSC a dynamic function within the marrow microenvironment (Prockop, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Typical MSC (obtained under long-term conditions) have been extensively studied both phenotipically (Allen and Dexter, 1984;Wilkins and Jones, 1995) and in their capacity to produce ECM molecules (Chichester et al, 1993;Verfaillie et al, 1994) and cytokines (Kittler et al, 1992). Most of these studies have been performed in cells maintained under culture conditions, including differentiation stimuli like horse serum and glucocorticoids.…”

mentioning

confidence: 99%

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Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells

1999

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Bone marrow stroma provides the microenvironment for hematopoiesis and is also the source of mesenchymal progenitors (mesenchymal or marrow stromal cells [MSC]) that may serve as long-lasting precursors for bone, cartilage, lung, and muscle. While several studies have indicated the differentiation potential of MSC, few studies have been performed on the cells themselves. In an attempt to further expand our knowledge on these cells, we have performed studies on their cell cycle, immuno- and adhesive-phenotype, ex vivo expansion, and differentiation properties. MSC cultures have been initiated from human bone marrow low-density mononuclear cells and maintained in the absence of differentiation stimuli and hematopoietic cells. The homogenous layer of adherent cells thus formed exhibits a typical fibroblastlike morphology, a population doubling time of 33 h, a large expansive potential, and cell cycle characteristics including a subset (20%) of quiescent cells. The antigenic phenotype of MSC is not unique, borrowing features of mesenchymal, endothelial, and epithelial cells. Together, MSC express several adhesion-related antigens, like the integrin subunits alpha4, alpha5, beta1, integrins alphavbeta3 and alphavbeta5, ICAM-1, and CD44H. MSC produce and functionally adhere to extracellular matrix molecules. When incubated under proper stimuli, MSC differentiate into osteoblasts or adipocytes. Taken together, these results demonstrate that adherent marrow-derived cells cultured in the absence of hematopoietic cells and differentiation stimulus give rise to a population of cells with phenotypical and functional features of mesenchymal progenitors. The existence of a subset of quiescent cells in MSC cultures seems to be extremely significant, since their number and properties should be enough to sustain a steady supply of cells that upon proliferation and commitment may serve as precursors for a number of nonhematopoietic tissues.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells

1999

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“…These are the markers of KER terminal differentiation and involucrin mostly expressed in the cornified layers of stratified squamous epithelium [27,30,31]. It has been shown that undifferentiated MSCs express collagen IV [32] and several epidermal keratin transcripts like keratin 8, 18 and 10 [5,33] even though the expression of keratin 8 and 10 at a protein level has not been found in undifferentiated MSCs [5], which indicates under certain specific conditions, MSCs may enter a developmental program that would commit it to an epidermal cell fate. Previous study has shown that under certain biomimic conditions in OC model, human MSCs were able to transdifferentiate into epidermis-like cells and express keratin 10 and filaggrin [24], whereas keratin 10, filaggrin and involucrin were not expressed in undifferentiated human MSCs [24,25].…”

Section: Epidermal Development and Homeostasis Regulated By Mscsmentioning

confidence: 99%

The dose effect of human bone marrow-derived mesenchymal stem cells on epidermal development in organotypic co-culture

Laco

Weber

et al. 2009

Journal of Dermatological Science

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“…There are also five genes for cytoskeleton-associated proteins: transgelin, α-B-crystallin, tropomyosin I, EPLIN, and RIL; four genes for soluble proteins: NNMT, PKCBP, P311, and PYCR1; and finally two nuclear genes: necdin and a LIM domain protein FHL2. It is noteworthy that among those genes, collagens types I, III, IV, and VI [27], transgelin [28], Thy-1 [29], and FAP [30] were known as characteristics of MSCs and previously identified in human MSCs.…”

Section: Differentially Expressed Genes In Ucb-derived Mscsmentioning

confidence: 99%

Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

Jeong¹,

Hong²,

Gang³

et al. 2005

STEM CELLS

104

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Mesenchymal stem cells (MSCs) retain both self-renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high-density oligonucleotide microarrays, we obtained the differential gene expression profile between a fraction of mononuclear cells of human umbilical cord blood (UCB) and its MSC subpopulation. Of particular interest was a subset of 47 genes preferentially expressed at 50-fold or higher in MSCs, which could be regarded as a molecular foundation of human MSCs. This subset contains numerous genes encoding collagens, other extracellular matrix or related proteins, cytokines or growth factors, and cytoskeleton-associated proteins but very few genes for membrane and nuclear proteins. In addition, a direct comparison of this microarray-generated transcriptome with the published serial analysis of gene expression data suggests that a molecular context of UCB-derived MSCs is more or less similar to that of bone marrow-derived cells. Altogether, our results will provide a basis for studies on molecular mechanisms controlling core properties of human MSCs. Stem Cells 2005;23:584-593

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Extracellular Matrix Gene Expression by Human Bone Marrow Stroma and by Marrow Fibroblasts

Cited by 49 publications

References 30 publications

Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells

Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells

The dose effect of human bone marrow-derived mesenchymal stem cells on epidermal development in organotypic co-culture

Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

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