Abstract:Decellularized mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla deriv… Show more
“…We agree with other groups suggesting using a cell carrier matrix to avoid immediate host rejection [26,28]. In addition to hydrogels , silk fibroin biomaterials , chitosan-gelatin-agarose or poly-caprolactone, an autologous fibrin matrix is a good candidate for cell transplantations [34,[69][70][71][72][73][74][75][76][77][78][79][80]. In this study, we evaluated the loading of a plasma-clot matrix with the purified OSCs for future treatment of SCI.…”
Cell therapies represent promising strategies to improve neurological functions after spinal cord injury
(SCI). Olfactory mucosa (OM) might be an attractive source of multipotent cells for neuroregeneration
because olfactory stem cells (OSCs) are resident. The regenerative capacity of OSCs has been demonstrated
in animal models and some clinical case reports. Up to now, there are no standard methods for purification,
characterization, and delivery of OSCs to the injury site. However, purification and characterization of the
grafted cells are prerequisites for clinical use to ensure maximum safety for the patients. In this study, we
isolated and purified OSCs from human OM using the neurosphere assay. Subsequently, the cells were
characterized, and the behavior of purified OSCs in a plasma clot was investigated. Our study demonstrated
that isolated cells from OM form neurospheres, which cells are positive for CD105 (98%) and CD90 (99%)
and negative for Epcam (<1%) and MUC5AC (<1%). Purified OSCs were positive for Nestin, CD44 as well
as GFAP and showed a lack of CD34 and CD45 expression. OSCs differentiated into neuron-like cells
expressing ß-III tubulin. However, differentiation into adipocytes, chondrocytes or osteoblast could not be
observed. In addition, OSCs stayed viable and were able to proliferate within the plasma clot. These results
highlight OSC as a candidate for autologous transplantation in combination with the plasma clot as a cell
carrier in SCI and neurodegenerative disease.
“…We agree with other groups suggesting using a cell carrier matrix to avoid immediate host rejection [26,28]. In addition to hydrogels , silk fibroin biomaterials , chitosan-gelatin-agarose or poly-caprolactone, an autologous fibrin matrix is a good candidate for cell transplantations [34,[69][70][71][72][73][74][75][76][77][78][79][80]. In this study, we evaluated the loading of a plasma-clot matrix with the purified OSCs for future treatment of SCI.…”
Cell therapies represent promising strategies to improve neurological functions after spinal cord injury
(SCI). Olfactory mucosa (OM) might be an attractive source of multipotent cells for neuroregeneration
because olfactory stem cells (OSCs) are resident. The regenerative capacity of OSCs has been demonstrated
in animal models and some clinical case reports. Up to now, there are no standard methods for purification,
characterization, and delivery of OSCs to the injury site. However, purification and characterization of the
grafted cells are prerequisites for clinical use to ensure maximum safety for the patients. In this study, we
isolated and purified OSCs from human OM using the neurosphere assay. Subsequently, the cells were
characterized, and the behavior of purified OSCs in a plasma clot was investigated. Our study demonstrated
that isolated cells from OM form neurospheres, which cells are positive for CD105 (98%) and CD90 (99%)
and negative for Epcam (<1%) and MUC5AC (<1%). Purified OSCs were positive for Nestin, CD44 as well
as GFAP and showed a lack of CD34 and CD45 expression. OSCs differentiated into neuron-like cells
expressing ß-III tubulin. However, differentiation into adipocytes, chondrocytes or osteoblast could not be
observed. In addition, OSCs stayed viable and were able to proliferate within the plasma clot. These results
highlight OSC as a candidate for autologous transplantation in combination with the plasma clot as a cell
carrier in SCI and neurodegenerative disease.
“…Cell therapy for neurological conditions may require integration with an appropriate biomaterial to support cells during transplantation and provide a structural support system post implantation. Recent investigations of ECM hydrogels for CNS applications have included the assessment of different source tissues to direct cell differentiation [96] and the transplantation of human neural stem cells embedded within ECM hydrogels to support the creation of de novo tissue [25]. Stem cells and primary cells have also been embedded within lung [49], liver [57], spinal cord [82], and adipose [50] ECM hydrogels to improve the tissue remodeling outcome.…”
Extracellular matrix (ECM) bioscaffolds prepared from decellularized tissues have been used to facilitate constructive and functional tissue remodeling in a variety of clinical applications. The discovery that these ECM materials could be solubilized and subsequently manipulated to form hydrogels expanded their potential in vitro and in vivo utility; i.e. as culture substrates comparable to collagen or Matrigel, and as injectable materials that fill irregularly-shaped defects. The mechanisms by which ECM hydrogels direct cell behavior and influence remodeling outcomes are only partially understood, but likely include structural and biological signals retained from the native source tissue. The present review describes the utility, formation, and physical and biological characterization of ECM hydrogels. Two examples of clinical application are presented to demonstrate in vivo utility of ECM hydrogels in different organ systems. Finally, new research directions and clinical translation of ECM hydrogels are discussed.
“…HL-ECM was solubilized by pepsin digestion as previously described [24]. Briefly, fragments of freeze-dried HL-ECM were incubated at a concentration of 10 mg/mL in a solution containing 1 mg/mL of pepsin and 0.01 M HCl, and stirred for 48 h at RT.…”
Section: Human Liver Tissue Decellularization and Preparation Of Solumentioning
The lack of robust methods to preserve, purify and in vitro maintain the phenotype of the human liver’s highly specialized parenchymal and non-parenchymal cell types importantly hampers their exploitation for the development of research and clinical applications. There is in this regard a growing interest in the use of tissue-specific extracellular matrix (ECM) to provide cells with an in vitro environment that more closely resembles that of the native tissue. In the present study, we have developed a method that allows for the isolation and downstream application of the human liver’s main cell types from cryopreserved material. We also isolated and solubilized human liver ECM (HL-ECM), analyzed its peptidomic and proteomic composition by mass spectrometry and evaluated its interest for the culture of distinct primary human liver cells. Our analysis of the HL-ECM revealed proteomic diversity, type 1 collagen abundance and partial loss of integrity following solubilization. Solubilized HL-ECM was evaluated either as a coating or as a medium supplement for the culture of human primary hepatocytes, hepatic stellate cells and liver sinusoidal endothelial cells. Whereas the solubilized HL-ECM was suitable for cell culture, its impact on the phenotype and/or functionality of the human liver cells was limited. Our study provides a first detailed characterization of solubilized HL-ECM and a first report of its influence on the culture of distinct human primary liver cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.