Extracellular matrix–dependent mechanosensing and mechanotransduction

Buwa, Natasha; Balasubramanian, Nagaraj

doi:10.1016/b978-0-323-90195-6.00019-x

Cited by 4 publications

(2 citation statements)

References 280 publications

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“…Cav1 depletion reduces the number of stress fibers, whereas Cav1 overexpression increases the fluorescence intensity of actin and its reorganization [ 179 , 180 ]. Among the proteins that regulate actin polymerization, the Rho small GTPases RhoA, Rac1, and Cdc42 play an essential role in its remodeling and many processes regulated by them, such as cell migration, division, and polarity [ 181 , 182 , 183 ]. Caveolae also regulate Rho GTPases.…”

Section: Interaction Between Caveolae and Cytoskeletonmentioning

confidence: 99%

Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

Sotodosos-Alonso

Pulgarín-Alfaro

Pozo

2023

Cells

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The plasma membrane (PM) is subjected to multiple mechanical forces, and it must adapt and respond to them. PM invaginations named caveolae, with a specific protein and lipid composition, play a crucial role in this mechanosensing and mechanotransduction process. They respond to PM tension changes by flattening, contributing to the buffering of high-range increases in mechanical tension, while novel structures termed dolines, sharing Caveolin1 as the main component, gradually respond to low and medium forces. Caveolae are associated with different types of cytoskeletal filaments, which regulate membrane tension and also initiate multiple mechanotransduction pathways. Caveolar components sense the mechanical properties of the substrate and orchestrate responses that modify the extracellular matrix (ECM) according to these stimuli. They perform this function through both physical remodeling of ECM, where the actin cytoskeleton is a central player, and via the chemical alteration of the ECM composition by exosome deposition. Here, we review mechanotransduction regulation mediated by caveolae and caveolar components, focusing on how mechanical cues are transmitted through the cellular cytoskeleton and how caveolae respond and remodel the ECM.

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Section: Interaction Between Caveolae and Cytoskeletonmentioning

confidence: 99%

Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

Sotodosos-Alonso

Pulgarín-Alfaro

Pozo

2023

Cells

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“…One or more mechanosensors can initiate various downstream signaling pathways in response to mechanical stimuli, allowing the cell to respond appropriately. Therefore, cell behaviors can be influenced by various physical characteristics such as stiffness or topology of the substrate 50 . The topology of the substrate is influenced by dynamics and organization of the actomyosin cytoskeleton 51 whereas stiffness can be attributed more toward the mechanical characteristics of the substrates and impact on cell behaviors [52][53][54] .…”

Section: Discussionmentioning

confidence: 99%

An inspired microenvironment of cell replicas to induce stem cells into keratocyte-like dendritic cells for corneal regeneration

Fallah Tafti,

Aghamollaei,

Moosazadeh Moghaddam

et al. 2023

Sci Rep

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Corneal stromal disorders due to the loss of keratocytes can affect visual impairment and blindness. Corneal cell therapy is a promising therapeutic strategy for healing corneal tissue or even enhancing corneal function upon advanced disorders, however, the sources of corneal keratocytes are limited for clinical applications. Here, the capacity of cell-imprinted substrates fabricated by molding human keratocyte templates to induce differentiation of human adipose-derived stem cells (hADSCs) into keratocytes, is presented. Keratocytes are isolated from human corneal stroma and grown to transmit their ECM architecture and cell-like topographies to a PDMS substrate. The hADSCs are then seeded on cell-imprinted substrates and their differentiation to keratocytes in DMEM/F12 (with and without chemical factors) are evaluated by real-time PCR and immunocytochemistry. The mesenchymal stem cells grown on patterned substrates present gene and protein expression profiles similar to corneal keratocytes. In contrast, a negligible expression of myofibroblast marker in the hADSCs cultivated on the imprinted substrates, is observed. Microscopic analysis reveals dendritic morphology and ellipsoid nuclei similar to primary keratocytes. Overall, it is demonstrated that biomimetic imprinted substrates would be a sufficient driver to solely direct the stem cell fate toward target cells which is a significant achievement toward corneal regeneration.

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