Extracellular matrix component signaling in cancer

Multhaupt, Hinke A.B.; Leitinger, Birgit; Gullberg, Donald; Couchman, John R.

doi:10.1016/j.addr.2015.10.013

Cited by 154 publications

(117 citation statements)

References 208 publications

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“…Moreover, by binding to heparin-binding sites of ECM components, sulfated GAGs collaborate with integrins for cell-ECM interactions in cell adhesion and migration1819. Sulfated GAGs are therefore essential regulators of cancer progression through modulation of cell differentiation, invasion and metastasis.…”

mentioning

confidence: 99%

Insights into the role of sulfated glycans in cancer cell adhesion and migration through use of branched peptide probe

Brunetti

Depau

Falciani

et al. 2016

Sci Rep

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The tetra-branched peptide NT4 selectively binds to different human cancer cells and tissues. NT4 specifically binds to sulfated glycosaminoglycans on cancer cell membranes. Since sulfated glycosaminoglycans are involved in cancer cell interaction with the extracellular matrix, we evaluated the effect of NT4 on cancer cell adhesion and migration. We demonstrated here that the branched peptide NT4 binds sulfated glycosaminoglycans with high affinity and with preferential binding to heparan sulfate. NT4 inhibits cancer cell adhesion and migration on different proteins, without modifying cancer cell morphology or their ability to produce protrusions, but dramatically affecting the directionality and polarity of cell movement. Results obtained by taking advantage of the selective targeting of glycosaminoglycans chains by NT4, provide insights into the role of heparan sulfate proteoglycans in cancer cell adhesion and migration and suggest a determinant role of sulfated glycosaminoglycans in the control of cancer cell directional migration.

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mentioning

confidence: 99%

Insights into the role of sulfated glycans in cancer cell adhesion and migration through use of branched peptide probe

Brunetti

Depau

Falciani

et al. 2016

Sci Rep

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“…26 The aberrant activation of the ECMreceptor interaction pathway is involved in cell survival, proliferation, migration, polarity, and differentiation. [27][28][29] The adhesion of cancer cells to ECM via collagen and integrin induced the activation of various pro-survival pathways such as PI3K/Akt, p53/MAPK, extracellular signal-regulated kinase (ERK)/MAPK, and Rho/ROCK conferring resistance to chemotherapeutic agents.…”

Section: Discussionmentioning

confidence: 99%

Identification of curcumin-inhibited extracellular matrix receptors in non–small cell lung cancer A549 cells by RNA sequencing

Zhang

et al. 2017

Tumour Biol.

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Curcumin is a potent anti-cancer drug in several types of human cancers. Despite of several preclinical and clinical studies of curcumin, the precise mechanism of curcumin in cancer prevention has remained unclear. In our study, we for the first time investigated whole transcriptome alteration in A549 non-small cell lung cancer (NSCLC) cell lines after treatment with curcumin using RNA sequencing. We found that lots of genes and signaling pathways were significantly altered after curcumin treatment in A549 cells. With bioinformatics approaches (gene ontology, Kyoto Encyclopedia of Genes and Genomes, and STRING), we found that those curcumin altered genes were not only the genes that induce cell death but also those extracellular matrix receptors and mitogen-activated protein kinase signaling pathway genes which regulate cell migration and proliferation. Among those significantly altered genes, eight genes (COL1A1, COL4A1, COL5A1, LAMA5, ITGA3, ITGA2B, DDIT3, and DUSP1) were further examined by quantitative reverse transcription polymerase chain reaction and western blot analysis in four non-small cell lung cancer cell lines. Both in cell lines and in mouse model, the extracellular matrix receptors including the integrin (ITGA3 and ITGA2B), collagen (COL5A1), and laminin (LAMA5) were significantly inhibited by curcumin at messenger RNA and protein levels. Functional studies confirmed that curcumin not only induced A549 cell death but also repressed cell proliferation and migration by regulating extracellular matrix receptors. Collectively, our study suggests that curcumin may be used as a promising drug candidate for intervening lung cancer in future studies.

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“…70 One of the most studied class of receptors are the integrins, which link ECM signals to intracellular events, and are often associated with the actin cytoskeleton. 71 Functional integrins are composed of two subunits: alpha and beta.…”

Section: Cell Recognition Of Collagenmentioning

confidence: 99%