Extracellular Matrix Bioscaffolds as Immunomodulatory Biomaterials<sup />

Dziki, Jenna L.; Huleihel, Luai; Scarritt, Michelle E.; Badylak, Stephen F.

doi:10.1089/ten.tea.2016.0538

Cited by 115 publications

(67 citation statements)

References 91 publications

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“…These scaffolds have been associated with a robust but favourable host immune response that triggers constructive remodelling outcomes and an overall anti-inflammatory environment. In particular, macrophages have been recognized as a critical determinant of regeneration after injury, tissue remodelling and cross-talk with endogenous stem/progenitor cells 59 , 60 .…”

Section: Discussionmentioning

confidence: 99%

Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors

et al. 2018

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A tissue engineered oesophagus could overcome limitations associated with oesophageal substitution. Combining decellularized scaffolds with patient-derived cells shows promise for regeneration of tissue defects. In this proof-of-principle study, a two-stage approach for generation of a bio-artificial oesophageal graft addresses some major challenges in organ engineering, namely: (i) development of multi-strata tubular structures, (ii) appropriate re-population/maturation of constructs before transplantation, (iii) cryopreservation of bio-engineered organs and (iv) in vivo pre-vascularization. The graft comprises decellularized rat oesophagus homogeneously re-populated with mesoangioblasts and fibroblasts for the muscle layer. The oesophageal muscle reaches organised maturation after dynamic culture in a bioreactor and functional integration with neural crest stem cells. Grafts are pre-vascularised in vivo in the omentum prior to mucosa reconstitution with expanded epithelial progenitors. Overall, our optimised two-stage approach produces a fully re-populated, structurally organized and pre-vascularized oesophageal substitute, which could become an alternative to current oesophageal substitutes.

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Section: Discussionmentioning

confidence: 99%

Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors

et al. 2018

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“…In addition, cells themselves manipulate their ECM microenvironment by synthesizing new matrix and modifying and digesting existing matrix, thereby altering its modulatory effects . ECM remodeling can expose previously hidden bioactive sequences or degrade intact bioactive sequences . Furthermore, digested soluble ECM fragments often modulate cell behavior in a manner that is distinct from the insoluble scaffold.…”

Section: Introductionmentioning

confidence: 99%

“…Given the role of the ECM in structural support of tissues, there has been significant effort in developing ECM‐based scaffolds for tissue engineering and regenerative medicine . For these applications, a biomaterial scaffold is often used to encourage the adhesion and infiltration of cells, and their differentiation and organization into functional tissues.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Matrix‐Based Strategies for Immunomodulatory Biomaterials Engineering

Rowley

Nagalla

Wang

et al. 2019

Adv Healthcare Materials

131

123

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The extracellular matrix (ECM) is a complex and dynamic structural scaffold for cells within tissues and plays an important role in regulating cell function. Recently it has become appreciated that the ECM contains bioactive motifs that can directly modulate immune responses. This review describes strategies for engineering immunomodulatory biomaterials that utilize natural ECM‐derived molecules and have the potential to harness the immune system for applications ranging from tissue regeneration to drug delivery. A top‐down approach utilizes full‐length ECM proteins, including collagen, fibrin, or hyaluronic acid‐based materials, as well as matrices derived from decellularized tissue. These materials have the benefit of maintaining natural conformation and structure but are often heterogeneous and encumber precise control. By contrast, a bottom‐up approach leverages immunomodulatory domains, such as Arg–Gly–Asp (RGD), matrix metalloproteinase (MMP)‐sensitive peptides, or leukocyte‐associated immunoglobulin‐like receptor‐1(LAIR‐1) ligands, by incorporating them into synthetic materials. These materials have tunable control over immune cell functions and allow for combinatorial approaches. However, the synthetic approach lacks the full natural context of the original ECM protein. These two approaches provide a broad range of engineering techniques for immunomodulation through material interactions and hold the potential for the development of future therapeutic applications.

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“…HAM has also been infiltrated by numerous leukocytes comprising macrophages. Recent studies have demonstrated that the extent of tissue regeneration after the implantation of a scaffold derived from the extracellular matrix depends on host tissue remodelling (Dziki, Huleihel, Scarritt, & Badylak, ). Macrophage polarization that plays an important role in this process can be dictated by the micro‐environment found in the scaffold, including cells (Brown, Valentin, Stewart‐Akers, McCabe, & Badylak, ).…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells seeded on a human amniotic membrane improve liver regeneration and mouse survival after extended hepatectomy

Despeyroux

Duret

Gondeau

et al. 2017

J Tissue Eng Regen Med

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Liver failure remains the leading cause of post-operative mortality after hepatectomy. This study investigated the effect of treatment with allogenic mesenchymal stem cells (MSCs) on survival and liver regeneration 48 hr and 7 days after 80% hepatectomy in C57Bl/6 mice. To optimize their biodistribution, MSCs were grown on acellular human amniotic membranes (HAM) and applied as a patch on the remnant liver. This approach was compared with MSC infusion and HAM patch alone. Hepatectomized mice without any treatment were used as control group. Survival rate was calculated and biological and histopathological parameters were analysed to monitor liver function and regeneration. MSCs grown on HAM retained their ability to proliferate, to differentiate into osteoblasts and adipocytes and to respond to pro-inflammatory stimuli. Extended hepatectomy (80%) led to liver failure that resulted in death within 72 hr in 76% of mice. MSC infusion showed an early but transitory positive effect on survival. MSC/HAM patches stimulated regeneration and significantly improved survival rate (54% vs. 24% in the control group at 7 days). They also decreased the severity of hepatectomy-induced steatosis, suggesting a modulation of lipid metabolism in hepatocytes. MSCs were still present on HAM at Days 2 and 7 posthepatectomy. In conclusion, engineered tissue constructs that combine MSCs and HAM improve survival and liver regeneration after 80% hepatectomy in mice. These encouraging results pave the way to potential clinical application.

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Extracellular Matrix Bioscaffolds as Immunomodulatory Biomaterials

Cited by 115 publications

References 91 publications

Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors

Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors

Extracellular Matrix‐Based Strategies for Immunomodulatory Biomaterials Engineering

Mesenchymal stem cells seeded on a human amniotic membrane improve liver regeneration and mouse survival after extended hepatectomy

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