Extracellular matrix‐based scaffolding technologies for periodontal and peri‐implant soft tissue regeneration

Tavelli, Lorenzo; McGuire, Michael K.; Zucchelli, Giovanni; Rasperini, Giulio; Feinberg, Stephen E.; Wang, Hom‐Lay; Giannobile, William V.

doi:10.1002/jper.19-0351

Cited by 111 publications

(168 citation statements)

References 69 publications

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“…Therefore, decellularized material, which is derived from growing cells, is a promising alternative. Only very few studies have described in vitro generated material for decellularization and most of them focused on periodontal tissue or blood vessel regeneration (Tavelli et al, 2019;Tondreau et al, 2015). Beside the material itself, the process of decellularization is also a critical step for decellularized tissue engineered material.…”

Section: Discussionmentioning

confidence: 99%

Decellularized human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction

Mertsch

Hasenzahl

Reichl

et al. 2020

J Tissue Eng Regen Med

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The shortage of donor corneas as well as the limitations of tissue substitutes leads to the necessity to develop alternative materials for ocular surface reconstruction. Corneal surface substitutes must fulfill specific requirements such as high transparency, low immunogenicity, and mechanical stability combined with elasticity. This in vitro study evaluates a decellularized matrix secreted from human corneal fibroblasts (HCF) as an alternative material for ocular surface reconstruction. HCF from human donors were cultivated with the supplementation of vitamin C to form a stable and thick matrix. Furthermore, due to enhanced cultivation time, a three‐dimensional like multilayered construct which partly mimics the complex structure of the corneal stroma could be generated. The formed human cell‐based matrices (so‐called cell sheets [CS]) were subsequently decellularized. The complete cell removal, collagen content, ultrastructure, and cell toxicity of the decellularized CS (DCS) as well as biomechanical properties were analyzed. Surgical feasibility was tested on enucleated porcine eyes. After decellularization and sterilization, a transparent, thick, cell free, and sterile tissue substitute resulted, which allowed expansion of limbal epithelial stem cells with no signs of cytotoxicity, and good surgical feasibility. DCS seem to be a promising new corneal tissue substitute derived from human cells without the limitation of donor material; however, future in vivo studies are necessary to further elucidate its potential for ocular surface reconstruction.

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Section: Discussionmentioning

confidence: 99%

Decellularized human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction

Mertsch

Hasenzahl

Reichl

et al. 2020

J Tissue Eng Regen Med

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“…Porcine-derived acellular collagen scaffolds have been successfully introduced in the field of periodontal regenerative and implant surgery as a viable alternative to autogenous connective tissue grafts [ 2 , 3 , 6 ]. The collagenous structure of the regenerative matrices under investigation in the present study contains cellular binding and activation motifs, which are supposed to enhance the recruitment, attachment, and subsequent activation of oral cells with different phenotypes [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…The physicochemical characteristics of the matrices can certainly affect their wound-healing properties [ 6 , 55 , 56 ]. A number of studies have demonstrated an impact of the matrix composition, porosity and pore size on the cellular attachment, proliferation, and motility.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, soft tissue graft harvesting requires a second surgical site, thus increasing (1) the patient morbidity, (2) the risk of damaging the palatal artery, (3) the surgical procedure time, (4) the risk of post-operative discomfort and complications, and finally (5) the risk of unpredictable esthetic outcome, e.g., differences in texture and color with adjacent tissues [ 4 , 5 ]. Collagen scaffolds of porcine origin offer a safe alternative to autogenous soft tissue grafts due to their natural origin and excellent biocompatibility, unlimited availability, easy processing, and low cost [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…The ideal soft tissue substitute is expected to perform a scaffolding function maintaining the biomechanical tissue integrity as well as to improve the natural wound-healing process by triggering its initiation and guiding its progression. On a cellular level, new generation matrices should trigger cellular migration to the wounded area as well as promote cellular adhesion and proliferation [ 6 ]. These cellular functions as part of the wound-healing process are rigorously regulated by matrix metalloproteinases (MMPs) and growth factors including TGF-β1, FGF-2, vascular endothelial growth factor-A (VEGF-A), and epidermal growth factor (EGF) [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Wound Healing Potential of Primary Human Oral Fibroblasts and Periodontal Ligament Cells Cultured on Four Different Porcine-Derived Collagen Matrices

et al. 2020

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Xenogenic collagen-based matrices represent an alternative to subepithelial palatal connective tissue autografts in periodontal and peri-implant soft tissue reconstructions. In the present study, we aimed to investigate the migratory, adhesive, proliferative, and wound-healing potential of primary human oral fibroblasts (hOF) and periodontal ligament cells (hPDL) in response to four commercially available collagen matrices. Non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) were all able to significantly enhance the ability of hPDL and hOF cells to directionally migrate toward the matrices as well as to efficiently repopulate an artificially generated wound gap covered by the matrices. Compared to NCM and DADM, CCM and HADM triggered stronger migratory response. Cells grown on CCM and HADM demonstrated significantly higher proliferative rates compared to cells grown on cell culture plastic, NCM, or DADM. The pro-proliferative effect of the matrices was supported by expression analysis of proliferative markers regulating cell cycle progression. Upregulated expression of genes encoding the adhesive molecules fibronectin, vinculin, CD44 antigen, and the intracellular adhesive molecule-1 was detected in hPDL and hOF cells cultured on each of the four matrices. This may be considered as a prerequisite for good adhesive properties of the four scaffolds ensuring proper cell–matrix and cell–cell interactions. Upregulated expression of genes encoding TGF-β1 and EGF growth factors as well as MMPs in cells grown on each of the four matrices provided support for their pro-proliferative and pro-migratory abilities. The expression of genes encoding the angiogenic factors FGF-2 and VEGF-A was dramatically increased in cells grown on DADM and HADM only, suggesting a good basis for accelerated vascularization of the latter. Altogether, our results support favorable influence of the investigated collagen matrices on the recruitment, attachment, and growth of cell types implicated in oral soft tissue regeneration. Among the four matrices, HADM has consistently exhibited stronger positive effects on the oral cellular behavior. Our data provide solid basis for future investigations on the clinical application of the collagen-based matrices in surgical periodontal therapy.

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PDGF‐BB‐enriched collagen matrix to treat multiple gingival recessions with the tunneled coronally advanced flap

Barootchi

Giannobile

Tavelli

2022

Clin Adv Periodontics

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Background: With technological advancements in reconstructive periodontology, traditional protocols for the treatment of gingival recessions (GRs) can be challenged. This manuscript presents preliminary findings of a novel minimallyinvasive approach for the regenerative treatment of multiple adjacent GR defects. Methods: Two healthy adults were treated as part of this study. Multiple adjacent GRs in both subjects (1 in the mandible, and 1 in the maxilla) were treated employing a tunneled coronally advanced flap (TCAF) design, with the application of a cross-linked collagen matrix (CCM) that was enriched with recombinant human platelet-derived growth factor-BB (PDGF-BB) that was also applied on the prepared root surfaces. Clinical, ultrasonographic, esthetic, and patient-reported outcomes were observed at approximately 6-and 18-month time points. Results: All sites healed uneventfully after the treatments. Complete root coverage was achieved and maintained throughout the follow-up observations, from 6 to 18 months. Patients reported minimal discomfort and reduction of dentinal hypersensitivity at the augmented sites. The areas augmented with CCM + PDGF-BB revealed an increased soft tissue thickness relative to baseline (pretreatment) measures, as well as reduction in the level of the facial bone dehiscences. Conclusion:This article describes the success of two cases of a novel minimally invasive regenerative approach for the treatment of multiple adjacent GR defects by the TCAF, using a CCM loaded with PDGF-BB. This approach offers potential as a minimally-invasive method to repair multiple adjacent GRs.

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Extracellular matrix‐based scaffolding technologies for periodontal and peri‐implant soft tissue regeneration

Cited by 111 publications

References 69 publications

Decellularized human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction

Decellularized human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction

Enhanced Wound Healing Potential of Primary Human Oral Fibroblasts and Periodontal Ligament Cells Cultured on Four Different Porcine-Derived Collagen Matrices

PDGF‐BB‐enriched collagen matrix to treat multiple gingival recessions with the tunneled coronally advanced flap

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