Extracellular Matrix as an Inductive Scaffold for Functional Tissue Reconstruction

Brown, Bryan N.; Badylak, Stephen F.

doi:10.1016/b978-0-12-800548-4.00002-4

Cited by 23 publications

(24 citation statements)

References 196 publications

(220 reference statements)

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“…2) demonstrated that an intact basement membrane complex was preserved on serous side. Basement membrane is a critical component of the ECM that supports and facilitates the growth of cells1248. Its distinct surface chemistry may modulate focal adhesion composition and signaling through changes in integrin binding, and different adhesive interactions activate various intracellular signaling pathways that direct cell cytokines release49.…”

Section: Discussionmentioning

confidence: 99%

“…A potentially ideal BP ECM scaffold should exhibit reduced antigenicity, while maintaining structure-function properties and recellularization potential111213. A range of approaches have been explored for reducing antigenicity of BP including decellularization (e.g., SDS, Triton X-100, trypsin)1415, enzymatic removal of α-Gal (i.e., α-galactosidase)16, and stepwise, solubilization-based antigen removal1718.…”

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confidence: 99%

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Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function

Liu

Wong

Griffiths

2016

Sci Rep

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Numerous studies have focused on generation of unfixed bovine pericardium (BP) extracellular matrix (ECM) for clinical application. However, the extent to which maintenance of native ECM niche is capable of directing behavior of repopulating cells remains relatively unexplored. By exploiting the sidedness of BP scaffolds (i.e., serous or fibrous surface), this study aims to determine the effect of ECM niche preservation on cellular repopulation using different scaffold generation methods. BP underwent either sodium dodecyl sulfate (SDS) decellularization or stepwise, solubilization-based antigen removal using amidosulfobetaine-14 (ASB-14). SDS scaffolds were toxic to repopulating human mesenchymal stem cells (hMSC). Scanning electron microscopy revealed distinct surface ultrastructure of ASB-14 scaffolds based on native BP sidedness. Basement membrane structures on the serous side stimulated hMSC cell monolayer formation, whereas fibrous side facilitated cell penetration into scaffold. Additionally, serous side seeding significantly increased hMSC adhesion and proliferation rate compared to the fibrous side. Furthermore, scaffold ECM niche stimulated sidedness dependent differential hMSC human leukocyte antigen expression, angiogenic and inflammatory cytokine secretion. This work demonstrates that ECM scaffold preparation method and preservation of BP side-based niches critically affects in vitro cell growth patterns and behavior, which has implications for use of such ECM biomaterials in clinical practice.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function

Liu

Wong

Griffiths

2016

Sci Rep

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“…Additionally, in applications where a scaffold or cellular construct provides mechanical support, early degradation may reduce the integrity of the host tissue or organ, potentially leading to further host injury. Scaffolds or constructs that persist for too long in the host may inhibit cellular remodeling, which can prevent integration and angiogenesis, and instead lead to encapsulation and scar formation [39]. Attempting to tune the in vivo degradation characteristics of natural materials through chemical methods (such as cross-linking) should be done with caution, as such chemically modified materials have been shown to produce undesirable host responses [12].…”

Section: Scaffold-host Interactionsmentioning

confidence: 99%

Physiologically inspired cardiac scaffolds for tailoredin vivofunction and heart regeneration

Kaiser

Coulombe²

2015

Biomed. Mater.

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Tissue engineering is well suited for the treatment of cardiac disease due to the limited regenerative capacity of native cardiac tissue and the loss of function associated with endemic cardiac pathologies, such as myocardial infarction and congenital heart defects. However, the physiological complexity of the myocardium imposes extensive requirements on tissue therapies intended for these applications. In recent years, the field of cardiac tissue engineering has been characterized by great innovation and diversity in the fabrication of engineered tissue scaffolds for cardiac repair and regeneration to address these problems. From early approaches that attempted only to deliver cardiac cells in a hydrogel vessel, significant progress has been made in understanding the role of each major component of cardiac living tissue constructs (namely cells, scaffolds, and signaling mechanisms) as they relate to mechanical, biological, and electrical in vivo performance. This improved insight, accompanied by modern material science techniques, allows for the informed development of complex scaffold materials that are optimally designed for cardiac applications. This review provides a background on cardiac physiology as it relates to critical cardiac scaffold characteristics, the degree to which common cardiac scaffold materials fulfill these criteria, and finally an overview of recent in vivo studies that have employed this type of approach.

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“…The extracellular matrix (ECM) that remains after lung decellularization is not an inert material, but rather a complex milieu of physical signals that encodes a ‘biochemical and mechanical language’ for resident cells. ECM-cell interactions govern cell viability, signaling pathways, proliferation, and differentiation [18–20]. Therefore, even seemingly minute changes in these matrix cues may induce positive outcomes such as cell engraftment and directed cell differentiation, or negative outcomes such as reduced cell attachment, cell death, inflammation, and cell-driven fibrotic matrix remodeling.…”

Section: Introductionmentioning

confidence: 99%

Production of decellularized porcine lung scaffolds for use in tissue engineering

Balestrini

Gard

Liu

et al. 2015

Integrative Biology

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There is a growing body of work dedicated to producing acellular lung scaffolds for use in regenerative medicine by decellularizing donor lungs of various species. These scaffolds typically undergo substantial matrix damage due to the harsh conditions required to remove cellular material (e.g., high pH, strong detergents), lengthy processing times, or pre-existing tissue contamination from microbial colonization. In this work, a new decellularization technique is described that maintains the global tissue architecture, key matrix components, mechanical composition and cell-seeding potential of lung tissue while effectively removing resident cellular material. Acellular lung scaffolds were produced from native porcine lungs using a combination of Triton X-100 and sodium deoxycholate (SDC) at low concentrations in 24 hours. We assessed the effect of matrix decellularization by measuring residual

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Extracellular Matrix as an Inductive Scaffold for Functional Tissue Reconstruction

Cited by 23 publications

References 196 publications

Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function

Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function

Physiologically inspired cardiac scaffolds for tailoredin vivofunction and heart regeneration

Production of decellularized porcine lung scaffolds for use in tissue engineering

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