Extracellular matrix affects mature biofilm and stress resistance of psychrotrophic spoilage Pseudomonas at cold temperature

Exploring the occurrence of Listeria in biofilms and deciphering the bacterial community in a frozen vegetable producing environment

“…The top five most abundant genera within the biofilm-positive sample group were Acinetobacter , Pseudomonas , Janthinobacterium , Brevundimonas , and Exiguobacterium . Different studies have demonstrated the biofilm-forming ability of all five of these genera ( Pantanella et al, 2007 ; Douterelo et al, 2014 ; Chauhan et al, 2018 ; Wagner et al, 2021 ; Chernogor et al, 2022 ; Gricajeva et al, 2022 ; Huang et al, 2022 ; Liu et al, 2023 ; Maifreni et al, 2023 ). In addition, Acinetobacter , Pseudomonas , Janthinobacterium , and Brevundimonas were previously found in biofilms in food processing environments ( Dzieciol et al, 2016 ; Wagner et al, 2020 ).…”

Application of cold-adapted microbial agents in soil contaminate remediation: biodegradation mechanisms, case studies, and safety assessments

“…37 With the decrease in temperature, cells produce a high level of extracellular polymeric substances exopolysaccharides (EPS), especially the secretion of extracellular proteins, preventing cell osmotic pressure damage and ice recrystallization. 38 Meanwhile, ice binding proteins (IBPs) such as antifreeze proteins (AFPs) and ice nuclear proteins (INPs) can inhibit the growth of ice crystals. There are multiple ice binding sites on the surface of AFPs to adsorb ice crystals, inhibit their recrystallization, reduce the freezing point of the solution, delay freezing or reduce the damage caused by freezing and thawing, and help cells survive at low temperatures, where INPs prevent the fast cooling of water by inducing ice crystallization at high subzero temperatures.…”

D-Limonene: Promising and Sustainable Natural Bioactive Compound

“…It has been widely reported that biofilms formed by fungi or bacteria are found on the surfaces of foods, medical equipment, soil, and other substrates, thus posing serious threats to public safety [101,102]. Biofilms are large numbers of microorganisms aggregated together, attached to the surface of organisms (e.g., meat tissue) or abiotic (e.g., processing equipment) [103] and enclosed in an extracellular matrix [104] (Figure 1). The biofilm matrix is generally composed of water and extracellular polymeric substances (EPSs), mainly composed of polysaccharides, proteins, nucleic acids, lipids, dead microbial cells [105,106]), flagella, and other adhesive fibers [103], thereby enhancing their resistance to drying, liquid flow, antimicrobial agents, disinfectants, and other methods [106].…”

“…Biofilms are large numbers of microorganisms aggregated together, attached to the surface of organisms (e.g., meat tissue) or abiotic (e.g., processing equipment) [103] and enclosed in an extracellular matrix [104] (Figure 1). The biofilm matrix is generally composed of water and extracellular polymeric substances (EPSs), mainly composed of polysaccharides, proteins, nucleic acids, lipids, dead microbial cells [105,106]), flagella, and other adhesive fibers [103], thereby enhancing their resistance to drying, liquid flow, antimicrobial agents, disinfectants, and other methods [106]. Biofilm formation is an adaptation strategy of microorganisms to their environment and it also increases their virulence, resulting in greater pathogenicity [107].…”