Extracellular matrices as advanced scaffolds for vascular tissue engineering

Piterina, Anna V.; Callanan, Anthony; Davis, Laura; Meaney, Claire L.; Walsh, Michael T.; McGloughlin, Timothy M.

doi:10.3233/bme-2009-0598

Cited by 17 publications

(15 citation statements)

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“…The most impressive results to date have been achieved by using fully natural TEVGs [16]; however, research to find a suitable extracellular matrix (ECM) scaffold for these types of grafts continues [16]. Some research has reported using porcine, goat and bovine tissue for TEVGs with diameters of 4–6 mm [6, 17, 18].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model

Cheng

et al. 2017

BioMed Eng OnLine

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BackgroundThe development of a suitable extracellular matrix (ECM) scaffold is the first step in vascular tissue engineering (VTE). Synthetic vascular grafts are available as an alternative to autologous vessels in large-diameter arteries (>8 mm) and medium-diameter arteries (6–8 mm). In small-diameter vessels (<6 mm), synthetic vascular grafts are of limited use due to poor patency rates. Compared with a vascular prosthesis, natural tissue ECM has valuable advantages. Despite considerable progress in recent years, identifying an optimal protocol to create a scaffold for use in small-diameter (<6 mm) fully natural tissue-engineered vascular grafts (TEVG), remains elusive. Although reports on different decellularization techniques have been numerous, combination of and comparison between these methods are scarce; therefore, we have compared five different decellularization protocols for making small-diameter (<6 mm) ECM scaffolds and evaluated their characteristics relative to those of fresh vascular controls.ResultsThe protocols differed in the choice of enzymatic digestion solvent, the use of non-ionic detergent, the durations of the individual steps, and UV crosslinking. Due to their small diameter and ready availability, rabbit arteria carotis were used as the source of the ECM scaffolds. The scaffolds were subcutaneously implanted in rats and the results were evaluated using various microscopy and immunostaining techniques.ConclusionsOur findings showed that a 2 h digestion time with 1× EDTA, replacing non-ionic detergent with double-distilled water for rinsing and the application of UV crosslinking gave rise to an ECM scaffold with the highest biocompatibility, lowest cytotoxicity and best mechanical properties for use in vivo or in situ pre-clinical research in VTE in comparison.Electronic supplementary materialThe online version of this article (doi:10.1186/s12938-017-0344-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model

Cheng

et al. 2017

BioMed Eng OnLine

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“…The optimal decellularization protocol should result in a strongly reduced/absent antigenicity by removing the cellular components of the donor while preserving the extracellular matrix, and therefore the physiological biomechanical strength. Most treatments for decellularization are based on chemical agents like detergents [3, 6, 7, 9, 12–14]. Detergent treatment is easy to use but can have a long treatment time, residual toxicity, and a decreased biomechanical stability, dependent on the type of tissue, chemical agent, the concentration, and the exposition time [15].…”

Section: Introductionmentioning

confidence: 99%

Pressure Shift Freezing as Potential Alternative for Generation of Decellularized Scaffolds

Eichhorn

Baier

Horst

et al. 2013

International Journal of Biomaterials

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Background. Protocols using chemical reagents for scaffold decellularization can cause changes in the properties of the matrix, depending on the type of tissue and the chemical reagent. Technologies using physical techniques may be possible alternatives for the production grafts with potential superior matrix characteristics. Material and Methods. We tested four different technologies for scaffold decellularization. Group 1: high hydrostatic pressure (HHP), 1 GPa; Group 2: pressure shift freezing (PSF); Group 3: pulsed electric fields (PEF); Group 4: control group: detergent (SDS). The degree of decellularization was assessed by histological analysis and the measurement of residual DNA. Results. Tissue treated with PSF showed a decellularization with a penetration depth (PD) of 1.5 mm and residual DNA content of 24% ± 3%. HHD treatment caused a PD of 0.2 mm with a residual DNA content of 28% ± .4%. PD in PEF was 0.5 mm, and the residual DNA content was 49% ± 7%. In the SDS group, PD was found to be 5 mm, and the DNA content was determined at 5% ± 2%. Conclusion. PSF showed promising results as a possible technique for scaffold decellularization. The penetration depth of PSF has to be optimized, and the mechanical as well as the biological characteristics of decellularized grafts have to be evaluated.

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“…As an acellularized biomaterial preparation, ECM does not appear to encapsulate when it is surgically implanted and appears to be remodeled into organized healthy tissue. [13][14][15][16][17][18][19][20][21] Remodeling is facilitated by cell migration and integration into the bioscaffold during the process of wound healing. CorMatrix is an acellular ECM product derived from porcine small intestine submucosa that is approved by the Food and Drug Administration for use in cardiovascular repairs.…”

Section: Discussionmentioning

confidence: 99%

“…An expanding repository of basic science and clinical literature suggests that this material possesses qualities that may make it optimal as an alternative to synthetic materials in vascular repair. [13][14][15][16][17][18][19][20][21] Specifically, the qualities include durable tensile strength capable of tolerating arterial pulsatile forces, the ability to resist thrombosis, and bacterial resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Research suggests that the use of ECMderived conduits is associated with improved antithrombogenic properties compared with synthetic alternatives. Piterina et al 18,19 noted that these conduits were superior in conduit hydrophilic characteristics, both at initial placement and throughout the regenerative process. 18,19 In particular, these researchers noted that superior endothelial reconstruction during remodeling resulted in a more optimal, naturally occurring antithrombogenic and blood-compatible surface compared with synthetic alternatives.…”

Section: Discussionmentioning

confidence: 99%

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Utility of a tubularized extracellular matrix as an alternative conduit for arteriovenous fistula aneurysm repair

DuBose

Fortuna

Charlton-Ouw

et al. 2016

Journal of Vascular Surgery

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Extracellular matrices as advanced scaffolds for vascular tissue engineering

Cited by 17 publications

References 0 publications

Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model

Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model

Pressure Shift Freezing as Potential Alternative for Generation of Decellularized Scaffolds

Utility of a tubularized extracellular matrix as an alternative conduit for arteriovenous fistula aneurysm repair

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