Extracellular lectins of saprophytyc bacteria Bacillus subtilis and it's mutants

Karpova, I. S.; Kovalenko, E. A.; Koretskaya, N. V.; Get’man, E. I.

doi:10.7124/bc.0006b1

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…However bacilli lectin genes are not yet installed. We identified an influence of gene functional condition of bacilli cells reparation system on bacteria lectin synthesis ability with using of mutants with defect reparation system [8,9]. It were established that the bacilli lectin activity depends on the recP gene product which was referred to postreplication reparation and recombination (Fig.…”

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confidence: 99%

Bacilli Lectins and Their Targets

Pidgorskyi¹,

Kovalenko²,

Sashchuk³

et al. 2017

Mikrobiol. Z.

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It were revealed structural features of extracellular sialic acid-spesific lectin from saprophytic strain of Bacillus subtilis IMV B-7014 and shown this lectin constituted the complex of molecular forms (isoforms, isolectins) that obtained the working names BSL1, BSL2 and BSL3 with mM 50 kDa, 40kDa and 55 kDa respectively. They differed in physical, chemical and biological characteristics. It were investigated direct and indirect impact mechanisms of the lectin and their molecular forms. We explored the lectin showed the greatest affinity to sialic acid-containing glycoconjugates where terminal O-acetylated sialic acid associated with subterminal D-galactose via α2,3-, α2,6-or α2,8-links. Bacilli lectins completely blocked surface sialic acid-containing receptors of influenza, herpes, hepatitis C viruses and HIV and thus prevented not only their adsorption and reproduction, but also appearance and further development of viral infection. It were shown multidirectional action of the lectin isoforms on proliferation of mammalian cell cultures with different origins: normal cells (primary mouse fibroblasts), relatively normal cells (epithelioid cells, Chinese hamster ovary) and cancer cell line of HeLa. We identified an influence of gene functional condition of reparation system of bacilli cells on ability to lectin synthesis by bacteria. Using the model system RNA-polymerase of bacteriophage T7 it were established the isolectins had different effects on the yield of RNA-transcription product in vitro.

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confidence: 99%

Bacilli Lectins and Their Targets

Pidgorskyi¹,

Kovalenko²,

Sashchuk³

et al. 2017

Mikrobiol. Z.

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“…Не па то ген ные ви ды Corynebacterium в этом отно ше нии не ис сле до ва ны. Сре ди сап ро фит ных бакте рий на и бо лее изуче ны лек ти ны ро да Bacillus, явля ю щи е ся внек ле точ ны ми си а лос пе ци фич ны ми глико по ли ме ра ми с ши ро ким спек тром би о ло гичес кой ак тив нос ти [13,14].…”

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The properties of lectins and cells surface biopolymers of non-pathogenic corynebacteria

et al. 2011

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Aim. To study lectin properties of non-pathogenic corynebacteria cells and preparations of their surface biopolymers (SBP), extracted by SDS. Methods. SBP were extracted from intact cells by 0.15 M solution of NaCl contains 1 % SDS. Protein content was determined using Lowry method, carbohydrates – with anthrone method. Electrophoresis was performed in SDS-PAGE according to Lemmli. Hemagglutinating activity (HAA) was studied using rabbit erythrocytes. The lectin carbohydrate specificity was determined by reaction of inhibition of hemagglutination. Results. Electrophoretic set of SBP preparations contained the proteins and carbohydrates biopolymers with molecular mass of 10.0–120.0 kDa which did not possess HAA. After extraction of SBP the corynebacteria cells remained viable and have HAA higher than intact cells (64–2048 units). The hemagglutinins of the majority of corynebacteria strains after treatment of cells with SDS exhibited the highest affinity to the bovine submandibular gland mucin and N-acetylneuraminic acid. Conclusions. The examined non-pathogenic strains of corynebacteria were found to contain the lectins, associated with internal layers of a cell wall, which showed a predominant specificity to sialic acids

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Extracellular lectins of saprophytyc bacteria Bacillus subtilis and it's mutants

Cited by 2 publications

References 2 publications

Bacilli Lectins and Their Targets

Bacilli Lectins and Their Targets

The properties of lectins and cells surface biopolymers of non-pathogenic corynebacteria

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