Extracellular Interception of Mutagens

Shankel, Delbert M.; Kuo, Simon; Haines, C. E.; Mitscher, Lester A.

doi:10.1007/978-1-4615-2984-2_5

Cited by 12 publications

(14 citation statements)

References 28 publications

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“…They are extensively studied for their antimicrobial, anticarcinogenic, and antiinflammatory properties [90]. In the field of chemoprevention, they show potential to both scavenge reactive oxygen species and form heterocomplexes with aromatic mutagens [91,92]. The latter mechanism, as described before, reduces mutagenic potential of aromatic mutagens by formation of molecular complexes with aromatic mutagens, thus reducing their bioavailability.…”

Section: Role Of Polyphenolsmentioning

confidence: 96%

Heterocyclic Aromatic Amines Heterocomplexation with Biologically Active Aromatic Compounds and Its Possible Role in Chemoprevention

2013

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Food-borne heterocyclic aromatic amines (HCAs) are known mutagens and carcinogens present especially in Western population diet, which contains large amount of meat and its products. HCAs are capable of interacting with DNA directly through the formation of covalent adducts, however this process requires biological activation in liver, mainly by cytochrome P450 enzymes. This process may produce mutations and in consequence may contribute to the development of cancer. However, there are many studies showing that several biologically active aromatic compounds (BACs) may protect against genotoxic effects of HCAs. Direct interactions and noncovalent heterocomplexes formation may be one of the most important mechanisms of such protection. This work describes several BACs present in human diet, which are capable of molecular complexes formation with HCAs and protect cells as well as whole organisms against HCAs action.

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Section: Role Of Polyphenolsmentioning

confidence: 96%

Heterocyclic Aromatic Amines Heterocomplexation with Biologically Active Aromatic Compounds and Its Possible Role in Chemoprevention

2013

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“…bindingrinactivation of damaging electrophiles; and d interference with the uptake of mutagens into the cells ŽSato et al, 1986;Shankel et al, 1993;Shimoi et al, . 1985 .…”

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confidence: 99%

Evaluation of antimutagenic and desmutagenic effects of humic and fulvic acids on root tips ofVicia faba

et al. 2000

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Humic acids HA and fulvic acids FA of different origin were evaluated for their antimutagenic and/ or desmutagenic activity on Vicia faba germinating seeds treated with the mutagen ( ) compound maleic hydrazide MH . Both HA and FA were tested at two different concentrations, 20 and 200 mg L y1 , either alone or together with 10 mg L y1 of MH. Two experimental procedures were used to investigate the occurrence and nature of the antimutagenic or desmutagenic activity. In procedure 1, HA or FA and MH were interacted for 24 h and then added to the seeds. In procedure 2, the seeds were first treated with HA or FA for 6 days and then transferred to plates containing the MH solution, so that no interaction occurred between HA or FA and MH outside the seeds. The evaluation of the genotoxic activity was done by counting both micronuclei and aberrant anatelophases in root tip cells. Results obtained by using procedure 1 showed a clear desmutagenic action of HA and FA used at both concentrations, whereas results of procedure 2 indicated no antimutagenic effect of HA or FA. ᮊ 2000 by John Wiley & Sons, Inc. Environ Toxicol 15: 513᎐517, 2000

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“…Antimutagenic studies using different auxotrophic Salmonella strains indicated that the TEO significantly inhibited the mutagenicity produced by all the direct acting mutagens, sodium azide, NPD, MNNG and tobacco and also inhibited the activation of 2-AAF by rat liver S9 fraction in a dose dependent manner. The antimutagenic property of essential oil may be due to inactivation of mutagens by inhibition of free radicals or activation of cellular antioxidant enzymes, inhibition of cytochrome p450 enzymes or activation of detoxification of mutagens (Shankel et al, 1993;Water et al, 1996;Ipek et al, 2005). TEO is also known to possess significant in vivo and in vitro antioxidant activity (Liju et al, 2011).…”

Section: Inhibition Of Cytochrome P450 Enzymes In Vitromentioning

confidence: 99%

Chemopreventive Activity of Turmeric Essential Oil and Possible Mechanisms of Action

Liju¹,

Jeena²,

Kuttan³

2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (16), 6575-6580 IntroductionMany human cancers are caused by chemical carcinogens, such as polycyclic aromatic hydrocarbons, heterocyclic amines, aromatic amines etc present in our environment. Continued exposure of these substances to human cells leads to genomic instability, including repair deficiency and accumulation of genetic alteration (Ames, 1983). Mutation is a major factor in carcinogenesis and incidence of cancer may be reduced by decreasing the rate of mutations induced by various chemical mutagens. Many carcinogens are activated through Phase I (cytochrome p450) enzymes present in endoplasmic reticulum of the liver cells. Induction of Phase II detoxification enzymes, such as glutathione S-transferase and UDP-glucuronyl transferase, is another mechanisms of protection against carcinogenesis (Piengchai et al., 2011). Modulating these enzymes may reduce cancer incidence.Spices which are widely used as food ingredient exhibits different pharmacological properties. Essential oils from spices are volatile compounds produced as secondary metabolites. The essential oil from Curcuma longa rhizome is a complex mixture obtained by steam distillation. A total number of 12 components of the essential oil are identified by GC-MS analysis and the principal compounds include ar-turmerone (61%), Department of Biochemistry, Amala Cancer Research Centre, Kerala, India *For correspondence: amalacancerresearch@gmail. com, amalaresearch@hotmail.com AbstractThis study aimed to evaluate the antimutagenic and anticarcinogenic activity of turmeric essential oil as well as to establish biochemical mechanisms of action. Antimutagenicity testing was accomplished using strains and known mutagens with and without microsomal activation. Anticarcinogenic activity was assessed by topical application of 7, 12 -dimethylbenz[a]anthracene (DMBA) as initiator and 1% croton oil as promoter for the induction of skin papillomas in mice. Inhibition of p450 enzymes by TEO was studied using various resorufins and aminopyrene as substrate. Turmeric essential oil (TEO) showed significant antimutagenic activity (p<0.001) against direct acting mutagens such as sodium azide (NaN 3 ), 4-nitro-O-phenylenediamine (NPD) and N-methyl-N-nitro N'nitrosoguanine (MNNG). TEO was found to have significant antimutagenic effect (>90%) against mutagen needing metabolic activation such as 2-acetamidoflourene (2-AAF). The study also revealed that TEO significantly inhibited (p<0.001) the mutagenicity induced by tobacco extract to Salmonella TA 102 strain. DMBA and croton oil induced papilloma development in mice was found to be delayed and prevented significantly by TEO application. Moreover TEO significantly (P<0.001) inhibited isoforms of cytochrome p450 (CYP1A1, CYP1A2, CYP2B1/2, CYP2A, CYP2B and CYP3A) enzymes in vitro, which are involved in the activation of carcinogens. Results indicated that TEO is antimutagenic and anticarcinogenic and inhibition of enzymes (p450) involved in the activation of carcinogen is one of its mecha...

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Extracellular Interception of Mutagens

Cited by 12 publications

References 28 publications

Heterocyclic Aromatic Amines Heterocomplexation with Biologically Active Aromatic Compounds and Its Possible Role in Chemoprevention

Heterocyclic Aromatic Amines Heterocomplexation with Biologically Active Aromatic Compounds and Its Possible Role in Chemoprevention

Evaluation of antimutagenic and desmutagenic effects of humic and fulvic acids on root tips ofVicia faba

Chemopreventive Activity of Turmeric Essential Oil and Possible Mechanisms of Action

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