Extracellular Hemoglobins from Annelids, and their Potential Use in Biotechnology

Abstract

This chapter highlights the diversity of invertebrate

Thermal stability of extracellular hemoglobin of Rhinodrilus alatus (HbRa): DLS and SAXS studies

“…As investigated by Zal et al (2000Zal et al ( , 2014, this diversity justifies the study of extracellular hemoglobin in different species of annelids. To evaluate different hemoglobin species is an interesting question, to test whether this diversity Scheme 1 Structural hierarchy of HbGp reflects evolutionary divergences or an intrinsic plasticity of the hemoglobin structure.…”

“…HbLt and HbGp are the most extensively studied giant hemoglobins (Bachega et al 2011;Carvalho et al 2012Carvalho et al , 2013Carvalho et al , 2015Krebs et al 1998;Oliveira et al 2007;Poli et al 2005;Royer et al 2005Royer et al , 2006Rousselot et al 2006;Santiago et al 2008Santiago et al , 2010a. Another widely studied giant protein is Arenicola marina hemoglobin (HbAm), orthologous of HbGp and HbLt, presenting quite similar hierarchic structural organization and biophysical properties (Zal and Rousselot 2014;Jernshøj et al 2013). These hemoglobins present high cooperativity in oxygen binding and high resistance to the oxidation process as compared to human hemoglobin (Poli et al 2005;Santiago et al 2008).…”

“…These hemoglobins present high cooperativity in oxygen binding and high resistance to the oxidation process as compared to human hemoglobin (Poli et al 2005;Santiago et al 2008). Due to these properties and the fact that they are extracellular hemoglobins with large size, HbLt and HbAm have been considered as a promising model system for developing therapeutic blood substitutes (artificial blood; Zal and Rousselot 2014).…”

“…To evaluate different hemoglobin species is an interesting question, to test whether this diversity Scheme 1 Structural hierarchy of HbGp reflects evolutionary divergences or an intrinsic plasticity of the hemoglobin structure. In addition, since to build extracellular hemoglobins different globin and linkers chains are necessary to achieve this particular quaternary architecture, as well as the stability of the oligomeric structure, these studies could allow to assess what are the essential elements for the success (Zal et al 2000;Zal and Rousselot 2014). Moreover, studies have been reported on some advances on biotechnological HbAm applications with the development of different products, as for example: a preservation organ solution and a restauration oxygenation blood, both based on the Arenicola marina hemoglobin (Zal and Rousselot 2014).…”

Newly Discovered Occurrences and Gene Tree of the Extracellular Globins and Linker Chains from the Giant Hexagonal Bilayer Hemoglobin in Metazoans

“…2007). Such features make HBL-Hb a promising candidate to act as a blood substitute in human transfusions/therapeutics and for the preservation of organs, tissues, and cells (Zal and Rousselot 2014; Zal et al 2014). HBL-Hb represents one of four groups of invertebrates extracellular Hbs, which comprised a multisubunit complex made of globins with a single oxygen-binding site in each one of them, and linker chains that are devoid of heme (Vinogradov 1985; Weber and Vinogradov 2001).…”