Extracellular heat shock protein 70 is a mortality predictor in patients with septic shock and is associated with the APACHE II and SOFA scores, and the pro‑inflammatory immune response

Bautista‐Carbajal, Patricia; Duarte‐Molina, Pablo; Contla‐Martínez, Indira Isamara; García‐León, Miguel Leonardo; Ángel-Ambrocio, Antonio H.; Baltazar‐López, Neyla; Wong‐Chew, Rosa María

doi:10.3892/wasj.2021.101

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 51 , 53 , 54 , 55 Notably, serum levels of HSP70 have been directly linked to mortality in patients with CSR and septic shock. 56 This suggests that the presence of HSP70 was a strong indicator of the outcome in this case.…”

Section: Discussionmentioning

confidence: 70%

“…There is also evidence that these HSPs play a protective role during neuroinflammation by suppressing astrocyte inflammatory signals or interacting with ASK1 and inhibiting the ASK1‐MKK4‐JNK pathway 51,53–55 . Notably, serum levels of HSP70 have been directly linked to mortality in patients with CSR and septic shock 56 . This suggests that the presence of HSP70 was a strong indicator of the outcome in this case.…”

Section: Discussionmentioning

confidence: 74%

See 1 more Smart Citation

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Smith

Brandehoff

Pepin

et al. 2023

Analytical Science Advances

View full text Add to dashboard Cite

Biofluid proteomics is a sensitive and high throughput technique that provides vast amounts of molecular data for biomarker discovery. More recently, dried blood spots (DBS) have gained traction as a stable, noninvasive, and relatively cheap source of proteomic data for biomarker identification in disease and injury. Snake envenomation is responsible for significant morbidity and mortality worldwide; however, much remains unknown about the systemic molecular response to envenomation and acquiring biological samples for analysis is a major hurdle. In this study, we utilized DBS acquired from a case of lethal rattlesnake envenomation to determine the feasibility of discovering biomarkers associated with human envenomation. We identified proteins that were either unique or upregulated in envenomated blood compared to non‐envenomated blood and evaluated if physiological response pathways and protein markers that correspond to the observed syndromes triggered by envenomation could be detected. We demonstrate that DBS provide useful proteomic information on the systemic processes that resulted from envenomation in this case and find evidence for a massive and systemic inflammatory cascade, combined with coagulation dysregulation, complement system activation, hypoxia response activation, and apoptosis. We also detected potential markers indicative of lethal anaphylaxis, cardiac arrest, and brain death. Ultimately, DBS proteomics has the potential to provide stable and sensitive molecular data on envenomation syndromes and response pathways, which is particularly relevant in low‐resource areas which may lack the materials for biofluid processing and storage.

show abstract

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 74%

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Smith

Brandehoff

Pepin

et al. 2023

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…Hsp70 has a dual role in the body depending on whether it is located intra-or extracellular, so that iHsp70 has a protective role and eHsp70 has a proinflammatory role. The study of eHsp70 has become relevant due to the availability of diagnostic kits for determining its concentration, and recent results show that it is a very useful predictor of mortality in patients with septic shock [16]. Hsp70 travels to the extracellular space in several ways: through leaking from necrotic cells, under the action of various stressors and inflammation in intact cells, it can be produced in the liver as an acute phase protein, and exosome transport and direct contact with the cell lipid membrane have been described [17,18].…”

Section: Introductionmentioning

confidence: 99%

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

et al. 2021

View full text Add to dashboard Cite

Metabolic stress in early lactation cows is characterized by lipolysis, ketogenesis, insulin resistance and inflammation because of negative energy balance and increased use of lipids for energy needs. In this study the relationship between lipid metabolite, lipid-based insulin resistance, and hepatocyte functionality indexes and tumor necrosis factor alpha (TNF-α) with extracellular heat shock protein 70 (eHsp70) was investigated. The experiment included 50 cows and all parameters were measured in blood serum. In cows with a more pronounced negative energy balance, the following was determined: a higher concentration of eHsp70, TNF-α, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHB), NEFA to insulin and NEFA to cholesterol ratio and lower concentration of cholesterol, very low-density lipoproteins (VLDL), low density lipoproteins (LDL) and liver functionality index (LFI). The eHsp70 correlated negatively with the values of cholesterol, VLDL, LDL, and triglycerides, while correlated positively with the level of NEFA and BHB. A higher concentration of eHsp70 suggests the development of fatty liver (due to a higher NEFA to cholesterol ratio and lower LFI) and insulin resistance (due to a lower revised quantitative insulin sensitivity check index RQUICKI-BHB and higher NEFA to insulin ratio). The eHsp70 correlated positively with TNF-α. Both TNF-α and eHsp70 correlated similarly to lipid metabolites. In cows with high eHsp70 and TNF-α values we found higher concentrations of NEFA, BHB, NEFA to insulin and NEFA to cholesterol ratio and a lower concentration of triglycerides and VLDL cholesterol compared to cows that had only high TNF-α values. Based on the positive correlation between eHsp70 and TNF-α, their similar relations, and the additional effect of eHsp70 (high TNF-α + eHsp70 values) on lipid metabolites we conclude that eHsp70 has pro-inflammatory effects implicating lipolysis, fatty liver, and fat tissue insulin resistance.

show abstract

Extracellular heat shock protein 70 is a mortality predictor in patients with septic shock and is associated with the APACHE II and SOFA scores, and the pro‑inflammatory immune response

Cited by 2 publications

References 0 publications

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Feasibility of detecting snake envenomation biomarkers from dried blood spots

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

Contact Info

Product

Resources

About