Extracellular glutathione recycling by γ-glutamyl transferase in barley root tip exposed to cadmium

Tamás, Ladislav; Alemayehu, Aster; Mistrı́k, Igor; Zelinová, Veronika

doi:10.1016/j.envexpbot.2015.05.013

Cited by 5 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the apoplastic space, however, it is rather unlikely that GSH functions as major anti-oxidant due to its relatively low abundance [ 20 ]. Nevertheless, it is clear from the literature that apoplastic GSH and its recycling fulfil other important roles that need further consideration [ 20 , 21 , 22 , 23 , 24 ]. The recycling of extracellular GSH (eGSH) is accomplished by the activity of γ-glutamyl transpeptidase (GGT) encoded by γ-GLUTAMYL TRANSPEPTIDASE 1 ( GGT1 ), that catabolises eGSH into its constituent amino acids [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Deckers

Hendrix

Prinsen

et al. 2020

IJMS

View full text Add to dashboard Cite

The toxic metal cadmium (Cd) is a major soil pollutant. Knowledge on the acute Cd-induced stress response is required to better understand the triggers and sequence of events that precede plant acclimation. Therefore, we aimed to identify the pressure points of Cd stress using a short-term exposure set-up ranging from 0 h to 24 h. Acute responses related to glutathione (GSH), hydrogen peroxide (H2O2), 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene and the oxidative challenge were studied at metabolite and/or transcript level in roots and leaves of Arabidopsis thaliana either exposed or not to 5 µM Cd. Cadmium rapidly induced root GSH depletion, which might serve as an alert response and modulator of H2O2 signalling. Concomitantly, a stimulation of root ACC levels was observed. Leaf responses were delayed and did not involve GSH depletion. After 24 h, a defined oxidative challenge became apparent, which was most pronounced in the leaves and concerted with a strong induction of leaf ACC synthesis. We suggest that root GSH depletion is required for a proper alert response rather than being a merely adverse effect. Furthermore, we propose that roots serve as command centre via a.o. root-derived ACC/ethylene to engage the leaves in a proper stress response.

show abstract

Section: Introductionmentioning

confidence: 99%

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Deckers

Hendrix

Prinsen

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…GGTs are the only enzymes to hydrolyze the gamma-glutamyl bond in GSH, and they are known to be involved in GSH recycling in extra-cytosolic (apoplastic and vacuolar) [48]. The expression level of GGTs were increased in Phormium tenax under water deficit stress [32], and the inhibition of GGT resulted in enhanced ROS generation in Cd-treated seedling [49]. Trentin et al [50] showed that ggt1 knockout mutant had higher ROS production compared to wild type Arabidopsis.…”

Section: Plos Onementioning

confidence: 99%

Time-course transcriptomic analysis of Petunia ×hybrida leaves under water deficit stress using RNA sequencing

et al. 2021

View full text Add to dashboard Cite

Water deficit limits plant growth and development, resulting in quality loss of horticultural crops. However, there is limited information on gene regulation and signaling pathways related to water deficit stress response at multiple time points. The objective of this research was to investigate global gene expression patterns under water deficit stress to provide an insight into how petunia (Petunia ×hybrida ‘Mitchell Diploid’) responded in the process of stress. Nine-week-old petunias were irrigated daily or placed under water stress by withholding water. Stressed plants reduced stomatal conductance after five days of water deficit, indicating they perceived stress and initiated stress response mechanisms. To analyze transcriptomic changes at the early stage of water deficit, leaf tissue samples were collected 1, 3, and 5 days after water was withheld for RNA sequencing. Under water deficit stress, 154, 3611, and 980 genes were upregulated and 41, 2806, and 253 genes were downregulated on day 1, 3, and 5, respectively. Gene Ontology analysis revealed that redox homeostasis processes through sulfur and glutathione metabolism pathways, and hormone signal transduction, especially abscisic acid and ethylene, were enriched under water deficit stress. Thirty-four transcription factor families were identified, including members of AP2/ERF, NAC, MYB-related, C2H2, and bZIP families, and TFs in AP2/ERF family was the most abundant in petunia. Interestingly, only one member of GRFs was upregulated on day 1, while most of TFs were differentially expressed on day 3 and/or 5. The transcriptome data from this research will provide valuable molecular resources for understanding the early stages of water stress-responsive networks as well as engineering petunia with enhanced water stress tolerance.

show abstract

Changes of non-protein thiols in root and organic acids in xylem sap involved in cadmium translocation of cadmium-safe rice line (Oryza Sative L.)

Guo

Zhang

et al. 2019

Plant Soil

View full text Add to dashboard Cite

Extracellular glutathione recycling by γ-glutamyl transferase in barley root tip exposed to cadmium

Cited by 5 publications

References 42 publications

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Time-course transcriptomic analysis of Petunia ×hybrida leaves under water deficit stress using RNA sequencing

Changes of non-protein thiols in root and organic acids in xylem sap involved in cadmium translocation of cadmium-safe rice line (Oryza Sative L.)

Contact Info

Product

Resources

About