Extracellular functions of 14-3-3 adaptor proteins

Kaplan, Andrew H.; Bueno, Mardja; Fournier, Alyson E.

doi:10.1016/j.cellsig.2016.12.007

Cited by 46 publications

(38 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation, combined with the fact that the presence of EV particles appears reduced in heat-treated Z. tritici cultures—a result seen in other fungi [ 28 , 42 , 43 ]—consolidates the hypothesis that Z. tritici EVs are actively secreted by cells rather than being randomly-shed membranous artefacts [ 44 ]. To this end, Z. tritici EVs also contain some proteins consistently associated with mammalian EVs, including the cytoskeletal components actin and tubulin; proteins implicated in signal transduction such as 14-3-3 domain-containing proteins; clathrin, which is involved in mammalian EV biogenesis as well as clathrin-mediated endocytosis; and heat-shock proteins, including Hsp70 family and Hsp90 proteins [ 21 , 45 , 46 ]. Taken together, the overlap of Z. tritici EV protein cargo with that of other fungal EVs and mammalian EVs provides confidence these are similar to the EVs released by other fungi.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Solomon

2020

Fungal Biol Biotechnol

View full text Add to dashboard Cite

Background The fungal pathogen Zymoseptoria tritici is a significant constraint to wheat production in temperate cropping regions around the world. Despite its agronomic impacts, the mechanisms allowing the pathogen to asymptomatically invade and grow in the apoplast of wheat leaves before causing extensive host cell death remain elusive. Given recent evidence of extracellular vesicles (EVs)—secreted, membrane-bound nanoparticles containing molecular cargo—being implicated in extracellular communication between plants and fungal pathogen, we have initiated an in vitro investigation of EVs from this apoplastic fungal wheat pathogen. We aimed to isolate EVs from Z. tritici broth cultures and examine their protein composition in relation to the soluble protein in the culture filtrate and to existing fungal EV proteomes. Results Zymoseptoria tritici EVs were isolated from broth culture filtrates using differential ultracentrifugation (DUC) and examined with transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). Z. tritici EVs were observed as a heterogeneous population of particles, with most between 50 and 250 nm. These particles were found in abundance in the culture filtrates of viable Z. tritici cultures, but not heat-killed cultures incubated for an equivalent time and of comparable biomass. Bottom-up proteomic analysis using LC–MS/MS, followed by stringent filtering revealed 240 Z. tritici EV proteins. These proteins were distinct from soluble proteins identified in Z. tritici culture filtrates, but were similar to proteins identified in EVs from other fungi, based on sequence similarity analyses. Notably, a putative marker protein recently identified in Candida albicans EVs was also consistently detected in Z. tritici EVs. Conclusion We have shown EVs can be isolated from the devastating fungal wheat pathogen Z. tritici and are similar to protein composition to previously characterised fungal EVs. EVs from human pathogenic fungi are implicated in virulence, but the role of EVs in the interaction of phytopathogenic fungi and their hosts is unknown. These in vitro analyses provide a basis for expanding investigations of Z. tritici EVs in planta, to examine their involvement in the infection process of this apoplastic wheat pathogen and more broadly, advance understanding of noncanonical secretion in filamentous plant pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Solomon

2020

Fungal Biol Biotechnol

View full text Add to dashboard Cite

show abstract

“…This observation, combined with the fact that the presence of EV particles appears reduced in heat-treated Z. tritici cultures -a result seen in other fungi [28,42,43] -consolidates the hypothesis that Z. tritici EVs are actively secreted by cells rather than being randomly-shed membranous artefacts [44]. To this end, Z. tritici EVs also contain some proteins consistently associated with mammalian EVs, including the cytoskeletal components actin and tubulin; proteins implicated in signal transduction such as 14-3-3 domain-containing proteins; clathrin, which is involved in mammalian EV biogenesis as well as clathrin-mediated endocytosis; and heat-shock proteins, including Hsp70 family and Hsp90 proteins [21,45,46]. Taken together, the overlap of Z. tritici EV protein cargo with that of other fungal EVs and mammalian EVs provides con dence these are similar to the EVs released by other fungi.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Solomon

2020

Preprint

View full text Add to dashboard Cite

Background: The fungal pathogen Zymoseptoria tritici is a significant constraint to wheat production in temperate cropping regions around the world. Despite its agronomic impacts, the mechanisms allowing the pathogen to asymptomatically invade and grow in the apoplast of wheat leaves before causing extensive host cell death remain elusive. Given recent evidence of extracellular vesicles (EVs) – secreted, membrane-bound nanoparticles containing molecular cargo – being implicated in extracellular communication between plants and fungal pathogen, we have initiated an in vitro investigation of EVs from this apoplastic fungal wheat pathogen. We aimed to isolate EVs from Z. tritici broth cultures and examine their protein composition in relation to the soluble protein in the culture filtrate and to existing fungal EV proteomes.Results: Zymoseptoria tritici EVs were isolated from broth culture filtrates using differential ultracentrifugation (DUC) and examined with transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). Z. tritici EVs were observed as a heterogeneous population of particles, with most between 50 – 250 nm. These particles were found in abundance in the culture filtrates of viable Z. tritici cultures, but not heat-killed cultures incubated for an equivalent time and of comparable biomass. Bottom-up proteomic analysis using LC-MS/MS, followed by stringent filtering revealed 240 Z. tritici EV proteins. These proteins were distinct from soluble proteins identified in Z. tritici culture filtrates, but were similar to proteins identified in EVs from other fungi, based on sequence similarity analyses. Notably, a putative marker protein recently identified in Candida albicans EVs was also consistently detected in Z. tritici EVs.Conclusion: We have shown EVs can be isolated from the devastating fungal wheat pathogen Z. tritici and are similar to protein composition to previously characterised fungal EVs. EVs from human pathogenic fungi are implicated in virulence, but the role of EVs in the interaction of phytopathogenic fungi and their hosts is unknown. These in vitro analyses provide a basis for expanding investigations of Z. tritici EVs in planta, to examine their involvement in the infection process of this apoplastic wheat pathogen and more broadly, advance understanding of noncanonical secretion in filamentous plant pathogens.

show abstract

“…They bind, acting as docking components, to phosphoserine/phosphothreonine residues of their “client” proteins and via this interaction they contribute to the activity, stability and localization of their binding partners . Due to these characteristics, they are involved in a constellation of cellular processes, such as signal transduction, cell cycle, proliferation, differentiation, apoptosis, metabolism, autophagy, acting mainly intracellularly but also extracellularly . Being involved in so many crucial cellular activities (many of them exhibiting tissue specificity), it is expected that members of the 14‐3‐3 family will have important contributions in several pathological processes and will constitute targets of choice for a variety of pharmaceutical interventions …”

Section: Discussionmentioning

confidence: 99%

The family of 14‐3‐3 proteins and specifically 14‐3‐3σ are up‐regulated during the development of renal pathologies

Rizou

Frangou

Marineli

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Chronic kidney disease, the end result of most renal and some systemic diseases, is a common condition where renal function is compromised due to fibrosis. During renal fibrosis, calreticulin, a multifunctional chaperone of the endoplasmic reticulum (ER) is up‐regulated in tubular epithelial cells (TECs) both in vitro and in vivo. Proteomic analysis of cultured TECs overexpressing calreticulin led to the identification of the family of 14‐3‐3 proteins as key proteins overexpressed as well. Furthermore, an increased expression in the majority of 14‐3‐3 family members was observed in 3 different animal models of renal pathologies: the unilateral ureteric obstruction, the nephrotoxic serum administration and the ischaemia‐reperfusion. In all these models, the 14‐3‐3σ isoform (also known as stratifin) was predominantly overexpressed. As in all these models ischaemia is a common denominator, we showed that the ischaemia‐induced transcription factor HIF1α is specifically associated with the promoter region of the 14‐3‐3σ gene. Finally, we evaluated the expression of the family of 14‐3‐3 proteins and specifically 14‐3‐3σ in biopsies from IgA nephropathy and membranous nephropathy patients. These results propose an involvement of 14‐3‐3σ in renal pathology and provide evidence for the first time that hypoxia may be responsible for its altered expression.

show abstract

Extracellular functions of 14-3-3 adaptor proteins

Cited by 46 publications

References 44 publications

Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

Extracellular vesicles from the apoplastic fungal wheat pathogen Zymoseptoria tritici

The family of 14‐3‐3 proteins and specifically 14‐3‐3σ are up‐regulated during the development of renal pathologies

Contact Info

Product

Resources

About