Extracellular facile biosynthesis, characterization and stability of gold nanoparticles byBacillus licheniformis

Abstract

The extracellular biosynthesis from CLS may serve as a suitable alternative for large scale synthesis of gold nanoparticles in vitro. The synthesis from lysed bacterial cell strongly suggests that exposure of microbial whole cells to the gold solution for nanoparticle formation is not necessary and that microorganism even in lysed state retained its bioreduction potential. Further the potential of biologically synthesized AuNPs as antimicrobial agents will be of great commercial importance.

In-vitro antimicrobial and anticancer properties of green synthesized gold nanoparticles using Anacardium occidentale leaves extract

“…Compared to the leaf extract, spectra of NP contains a reduced peak at 1300 cm −1 , which represents C—O group. Thus, the C—O group in the plant leaves extract of A. occidentale has contributed to the formation of AuNPs and indicate the presence and binding of phenolic groups to the synthesized NP (Singh et al, 2014, Patil et al, 2016). Therefore, the investigation advice that phytochemical compounds (phenols, flavonoids) present in A. occidentale extract promotes the generation of AuNPs (Keshavamurthy et al, 2018, Herizchi et al, 2016).…”

“…The lyophilized extract powder (0.5 g) was reconstituted in double distilled water. To the 1 ml of A. occidentale extract, 20 ml of chloroauric acid (0.01 M) was added drop wise under continuous stirring (500 rpm) on a magnetic stirrer and the whole mixture was incubated overnight (Singh et al, 2014). The color change (yellow to ruby red) visible after the incubation period and was subjected for spectroscopic and bioactivity study.…”

Biosynthesis and antibiotic activity of silver nanoparticles using different sources: Glass industrial sewage‐adapted Bacillus sp. and herbaceous Amaranthus sp

“…Similarly, other studies have demonstrated the ability of Bacillus spp. to bioreduce various metallic ions, including Ag [10], Au [25], and Pd [26] ions. It is well understood that effluents of glass industries contain toxic metals because of the fact that a number of raw materials, refining chemicals, and coloring agents are usually applied in a glass-manufacturing unit [15].…”

Multifunctional fluorescent titania nanoparticles: green preparation and applications as antibacterial and cancer theranostic agents

“…The isolated luminescent Vibrio sp. VLA was inoculated in sterile sea water complete medium (SWC, 2.4% w/v sea salt, 0.5% w/v peptone, 0.3% w/v yeast extract and 0.3% v/v glycerol) and incubated for 16 h at 28 C. The cell lysate supernatant (CLS) was obtained using previous method [8] with slight modification. Briefly, the bacterial medium was separated using centrifugation, the bacterial cells (pellet) were resuspended in sterile distilled water (SDW), vortexed gently and sonicated for 10 min to lyse.…”