Extracellular enzymes produced by microorganisms isolated from maritime Antarctica

Loperena, Lyliam; Soria, Verónica Carla Abenza; Varela, Hermosinda; Lupo, Sandra; Bergalli, Alejandro; Guigou, Mairan; Pellegrino, Andrés; Bernardo, Angela; Calviño, Ana Alejandra; Andrade‐Rivas, Federico; Batista, Silvia

doi:10.1007/s11274-012-1032-3

Cited by 90 publications

(52 citation statements)

References 27 publications

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“…Antarctic bacteria have been reported to pro− duce a range of polymer degrading enzymes (Kumar et al 2011;Loperena et al 2012;Tropeano et al 2012), but few studies on ribonuclease and phytase produc− tion by Antarctic bacteria have been reported (Reddy et al 1994;Park and Cho 2011). Detection of a variety of biopolymer−degrading enzymes produced by the tested Antarctic bacteria suggested their key role in the degradation of the organic matter in their natural environments.…”

Section: Discussionmentioning

confidence: 99%

“…Psychrophilic and psychrotolerant microorganisms have developed various adaptations to extreme environments through physiological and ecological mecha− nisms (Russell 1998;De Maayer et al 2014). Enzymes are an essential target for adaptation of microorganisms to extreme environments with considerable poten− tial for industrial application (Feller and Gerday 2003;Cavicchioli et al 2011;Kumar et al 2011;Gesheva and Vasileva−Tonkova 2012;Loperena et al 2012;Tropeano et al 2012). Moreover, Antarctic bacteria may develop ecologically im− portant capabilities in response to the impact of different stresses such as halo− tolerance, metal tolerance and antibiotic resistance, which provide them with se− lective advantages.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Tomova¹,

Stoilova−Disheva²,

Vasileva−Tonkova³

2014

Polish Polar Research

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Abstract:In this study, selected heavy metals resistant heterotrophic bacteria isolated from soil samples at the Windmill Islands region, Wilkes Land (East Antarctica), were character− ized. Phylogenetic analysis revealed affiliation of isolates to genera Bacillus, Lysini− bacillus, Micrococcus and Stenotrophomonas. The strains were found to be psychrotolerant and halotolerant, able to tolerate up to 10% NaCl in the growth medium. The Minimum In− hibitory Concentration of the seven heavy metals Cr, Cu, Ni, Co, Cd, Zn, and Pb was deter− mined in solid media for each bacterial strain. Gram−positive Vi−2 strain and Gram−negative Vi−4 strain showed highest multiply heavy metals resistance, and Vi−3 and Vi−4 strains showed multi−antibiotic resistance to more than a half of the 13 used antibiotics. Plasmids were detected only in Gram−negative Vi−4 strain. The bacteria were able to produce differ− ent hydrolytic enzymes including industrially important proteases, xylanases, cellulases, and b−glucosidases. High heavy metals resistance of the Antarctic bacteria suggests their potential application for wastewater treatment in cold and temperate climates. Highly sensi− tive to Cd and Co ions Vi−1, Vi−5 and Vi−7 strains would be promising for developing biosensors to detect these most toxic heavy metals in environmental samples.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Tomova¹,

Stoilova−Disheva²,

Vasileva−Tonkova³

2014

Polish Polar Research

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“…The optimum growth temperature for the psychrophiles is below 15°C; however, for psychrotrophs it is about 20-25°C [20]. These organisms are located in the cold regions of the Earth including polar zones, high mountains, glaciers, and deep oceans along with exteriors of flora and fauna surviving in cold atmospheres [21][22][23][24][25][26][27]. Psychrophilic microorganisms, including bacteria (e.g.…”

Section: Source Of Cold-active Enzymesmentioning

confidence: 99%

Cold-active enzymes in food biotechnology: An updated mini review

2018

J App Biol Biotech

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Cold-active enzymes and their anticipated application in various industries including food industry attracted attention of worldwide scientific community. Cold-active enzymes, also known as psychrophilic enzymes, possess high catalytic activity at low and moderate temperatures. Due to low-temperature activity, these enzymes utilize less energy in biochemical reactions and also stabilize fragile compounds in the reaction medium. The source of coldactive enzymes is basically psychrophilic/psychrotrophic microorganisms which are found in cold environments. In comparison to mesophilic and thermophilic enzymes, till date, very few cold-active enzymes are known and least explored so far in the food industry. This review contains latest development and innovation in cold-active enzymes along with their applications in food biotechnology.

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“…Antarctic environments can sustain a large diversity of well-adapted microorganisms known as psychrophiles or psychrotrophs (Loperena et al 2012). Compounds and enzymes produced by these microorganisms have the capability and advantage for technological use due to higher enzymatic activities at lower temperatures than their mesophilic and thermophilic counterparts (Loperena et al 2012;Muffler et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Compounds and enzymes produced by these microorganisms have the capability and advantage for technological use due to higher enzymatic activities at lower temperatures than their mesophilic and thermophilic counterparts (Loperena et al 2012;Muffler et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities

Lavín¹,

Átala²,

Gallardo-Cerda³

et al. 2016

Polish Polar Research

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Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17 ºC) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures.

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Extracellular enzymes produced by microorganisms isolated from maritime Antarctica

Cited by 90 publications

References 27 publications

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica

Cold-active enzymes in food biotechnology: An updated mini review

Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities

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