Extracellular DNA as a target for biofilm control

Okshevsky, Mira; Regina, Viduthalai R.; Meyer, Rikke Louise

doi:10.1016/j.copbio.2014.12.002

Cited by 217 publications

(191 citation statements)

References 72 publications

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“…The ECM is hydrated and therefore is composed primarily of water. The matrix that supports the gel is composed of structural proteins and polysaccharides and contains extracellular DNA (eDNA) (145)(146)(147)(148)(149)(150)(151). Recently, McCrate et al (152) demonstrated that the ECM of E. coli biofilms contains cellulose and curli proteins, with the latter forming fibrous amyloid bodies.…”

Section: Defining a C Albicans Biofilm: Lessons From Bacteriamentioning

confidence: 99%

Plasticity of Candida albicans Biofilms

Soll

Daniels

2016

Microbiol Mol Biol Rev

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SUMMARYCandida albicans, the most pervasive fungal pathogen that colonizes humans, forms biofilms that are architecturally complex. They consist of a basal yeast cell polylayer and an upper region of hyphae encapsulated in extracellular matrix. However, biofilms formedin vitrovary as a result of the different conditions employed in models, the methods used to assess biofilm formation, strain differences, and, in a most dramatic fashion, the configuration of the mating type locus (MTL). Therefore, integrating data from different studies can lead to problems of interpretation if such variability is not taken into account. Here we review the conditions and factors that cause biofilm variation, with the goal of engendering awareness that more attention must be paid to the strains employed, the methods used to assess biofilm development, every aspect of the model employed, and the configuration of theMTLlocus. We end by posing a set of questions that may be asked in comparing the results of different studies and developing protocols for new ones. This review should engender the notion that not all biofilms are created equal.

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Section: Defining a C Albicans Biofilm: Lessons From Bacteriamentioning

confidence: 99%

Plasticity of Candida albicans Biofilms

Soll

Daniels

2016

Microbiol Mol Biol Rev

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“…To target biofilm formation to inhibit persistence and recurrent infection, substances are being investigated that can inhibit secretion of biofilm components, biofilm matrix formation or destroy or resolve existing biofilm matrices. Furthermore, DNase I can degrade the extracellular DNA present in the biofilm matrix (Okshevsky et al 2015;Qin et al 2007), interfering with its formation and stability as shown for Bordetella pertussis (Conover et al 2011), Listeria monocytogenes (Harmsen et al, 2010), and Campylobacter jejuni (Brown et al 2015). A bacterial glycoside hydrolase, named Dispersin B, has been isolated from Actinobacillus actinomycetemcomitans, which was shown to disrupt mature Actinobacillus biofilms (Rasko et al 2008;Stephenson et al 2000;Wilke et al 2015;Worthington et al 2013).…”

Section: Prevention and Resolution Of Biofilmsmentioning

confidence: 99%

Anti-virulence Strategies to Target Bacterial Infections

Mühlen

Dersch

2015

Current Topics in Microbiology and Immunology

133

139

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Resistance of important bacterial pathogens to common antimicrobial therapies and the emergence of multidrug-resistant bacteria is increasing at an alarming rate and constitutes one of our greatest challenges in the combat of bacterial infection and accompanied diseases. Given the current shortage of effective drugs, lack of successful prevention measures and only a few new antibiotics in the clinical pipeline demands the development of novel treatment options and alternative antimicrobial therapies. Our increasing understanding of bacterial virulence strategies and the induced molecular pathways of the infectious disease provide novel opportunities to target and interfere with crucial pathogenicity factors or virulence-associated traits of the bacteria while bypassing the evolutionary pressure on the bacterium to develop resistance. In the past decade, numerous new bacterial targets for anti-virulence therapies have been identified, and structure-based tailoring of intervention strategies as well as screening assays for small molecule inhibitors of such pathways were successfully established. In this chapter, we will take a closer look at the bacterial virulence-related factors and processes that present promising targets for antivirulence therapies, recently discovered inhibitory substances and their promises and discuss the challenges and problems that have to be faced.

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“…In S. aureus, S. epidermidis, V. cholerae, and P. aeruginosa PAO1, the DNasedriven disruption of established biofilms was dependent on biofilm age; young biofilms were more sensitive to DNase than older biofilms (102)(103)(104)(105). Thus, eDNA is important for the structure of the young biofilm but its role is later taken over by other exopolymers (101). The chemical nature of the long, charged DNA molecule is thought to modulate the cell surface properties and to promote cell-to-cell and cell-to-surface adhesion (100).…”

Section: Ecm Components In Bacterial Biofilmsmentioning

confidence: 99%

“…Extracellular genomic DNA (eDNA) was found to be an important structural component in many bacterial biofilms (99,100). Addition of DNase to growing or mature biofilms of various bacterial species results in inhibition of biofilm formation or disruption of the established biofilms (101). In S. aureus, S. epidermidis, V. cholerae, and P. aeruginosa PAO1, the DNasedriven disruption of established biofilms was dependent on biofilm age; young biofilms were more sensitive to DNase than older biofilms (102)(103)(104)(105).…”

Section: Ecm Components In Bacterial Biofilmsmentioning

confidence: 99%

The Matrix Reloaded: How Sensing the Extracellular Matrix Synchronizes Bacterial Communities

Steinberg

Kolodkin‐Gal

2015

J Bacteriol

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In response to chemical communication, bacterial cells often organize themselves into complex multicellular communities that carry out specialized tasks. These communities are frequently referred to as biofilms, which involve the collective behavior of different cell types. Like cells of multicellular eukaryotes, the biofilm cells are surrounded by self-produced polymers that constitute the extracellular matrix (ECM), which binds them to each other and to the surface. In multicellular eukaryotes, it has been evident for decades that cell-ECM interactions control multiple cellular processes during development. While cells both in biofilms and in multicellular eukaryotes are surrounded by ECM and activate various genetic programs, until recently it has been unclear whether cell-ECM interactions are recruited in bacterial communicative behaviors. In this review, we describe the examples reported thus far for ECM involvement in control of cell behavior throughout the different stages of biofilm formation. The studies presented in this review have provided a newly emerging perspective of the bacterial ECM as an active player in regulation of biofilm development.

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Extracellular DNA as a target for biofilm control

Cited by 217 publications

References 72 publications

Plasticity of Candida albicans Biofilms

Plasticity of Candida albicans Biofilms

Anti-virulence Strategies to Target Bacterial Infections

The Matrix Reloaded: How Sensing the Extracellular Matrix Synchronizes Bacterial Communities

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