Extracellular depolymerization of hyaluronic acid in cultured human skin fibroblasts

Nakamura, Toshiya; Takagaki, Keiichi; Kubo, Kohmei; Morikawa, Akiko; Tamura, Shinji; Endo, Masahiko

doi:10.1016/s0006-291x(05)80174-3

Cited by 27 publications

(13 citation statements)

References 16 publications

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“…Therefore, the depolymerization of radioactive [14C]hyaluronate was investigated in medium derived from cervix and skin fibroblasts. It was found that the hyaluronate of high molecular weight in the medium of skin fibroblasts was depolymerized to units of Mr about 40,000, corresponding to the previous report of Nakamura et al [4] (Fig. 1A).…”

Section: Discussionsupporting

confidence: 87%

“…In order to resolve this issue, changes in the molecular weight of hyaluronate during fibroblast incubation and the effects of hormones on the hyaluronate depolymerization by cultured human uterine cervix fibroblasts were investigated. It was found that hyaluronate depolymerization activity corresponding to that reported by Nakamura et al [4] was induced by progesterone in the cultured fibroblasts derived from human uterine cervix.…”

Section: Introductionsupporting

confidence: 70%

“…Therefore, it seems that progesterone binds to progesterone receptors in cervix fibroblasts and induces a novel endo-fl-N-acetylglucosaminidase, which is different from the hyaluronidase. The mechanism responsible for this induction is still not clear, but the enzyme may be similar to the endo-fl-Nacetylglucosaminidase reported by Nakamura et al [4]. It is likely that further study of the depolymerizing enzyme induced by progesterone will help to elucidate the mechanisms of hyaluronate depolymerization and its regulation in the connective tissue of the human uterine cervix after parturition.…”

Section: Discussionmentioning

confidence: 52%

“…Nakamura et al recently reported that highmolecular-weight hyaluronate was depolymerized to a constant molecular weight (Mr = 40,000) in the culture medium, and that the reducing terminal of the depolymerized molecule was a newly exposed N-acetylglucosamine [4]. Thus the presence of a hyaluronate-depolymerizing enzyme, endo-fl-N-acetylglucosaminidase, was demonstrated in cultured human skin fibroblasts.…”

Section: Introductionmentioning

confidence: 98%

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Hyaluronate depolymerization activity induced by progesterone in cultured fibroblasts derived from human uterine cervix

et al. 1994

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AlmtractHigh-molecular-weight [t4C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated.[14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (Mr about 40,000), whereas that of cervix fibroblasts was not depolymerized, irrespective of incubation period. However, when progesterone was added to the medium of cervix fibroblasts, hyaluronate was depolymerized to the same extent as that in skin fibroblasts. The reducing terminal sugar of the depolymerized hyaluronate was N-aeetylglucosamine. These results suggest that a hyaluronate-depolymerizing enzyme, endo-,&N-acetylglucosaminidase, was induced by progesterone in cultured fibroblasts derived from human uterine cervix.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 98%

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Hyaluronate depolymerization activity induced by progesterone in cultured fibroblasts derived from human uterine cervix

et al. 1994

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“…were cultured in Eagle's minimum essential medium containing 10% fetal bovine serum in 5% C02-95% air at 37~ as described previously (3). Cells at growth phase were cultured for 12 h in the absence or presence of 0.5 mM MU.…”

Section: Introductionmentioning

confidence: 99%

Effect of 4‐methylumbelliferone on cell‐free synthesis of hyaluronic acid

et al. 1997

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Summary: Human skin fibroblasts were cultured in the presence of 0.5 mM 4-methylumbelliferone for 12 h, and cell-free synthesis of hyaluronic acid was performed using membrane-rich fraction from the ceils. The preincubation of the cells with 4-mcthylumbelliferone reduced hyaluronic acid synthesis to 15% of that of non-preincubated cells, although its chain length was not changed. On the other hand, without preincubation of the cells with 4-methylumbelliferone, hyaluronic acid synthesis was not changed even when 4-methylumbelliferone was added directly to the reaction mixture. These results suggest that 4-methylumbelliferone represses the expression of hyaluronic acid synthase on the cell surface.

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