Extracellular Cell Wall Lytic Enzyme from
            <i>Staphylococcus aureus:</i>
            Purification and Partial Characterization

Huff, Eskin; Silverman, Carol S.; Adams, Nancy J.; Awkard, Woodruff S.

doi:10.1128/jb.103.3.761-769.1970

Cited by 30 publications

(6 citation statements)

References 19 publications

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“…It is unknown whether the activities of the separation enzyme and lytic enzyme of the cell wall are due to the same enzyme in S. aureus. Recently Chatterjee et al (3) reported on a mutant of S. aureus that is deficient in N-acetylmuramyl-L-alanine amidase, which is the major lytic enzyme of the cell wall in S. aureus (8). They reported that the mutant formed large cell clumps, but they did not describe the arrangement of cells in the clumps.…”

Section: Discussionmentioning

confidence: 99%

Formation of regular packets of Staphylococcus aureus cells

Koyama¹,

Yamada²,

Matsuhashi³

1977

J Bacteriol

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Staphylococcus aureus, which usually forms grape-like clusters, has the ability to form regularly arranged cell packets. These regular cell packets are formed when the activity of its separation enzyme(s) is lost either by treatment with detergents, such as sodium dodecyl sulfate or Trition X-100, or by mutation of the cells. These cell packets consisted of 8 to 64 spherical cells that have a three-dimensional arrangement. Some irregularity in the arragement of cells in packets, however, can be observed by scanning electron microscopy. It is concluded that S. aureus fundametally divides along three definitely oriented planes that are located at right angles to each other. After cell division, the cells usually become translocated due to the action of a separation enzyme(s) to form grape-like clusters.

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Section: Discussionmentioning

confidence: 99%

Formation of regular packets of Staphylococcus aureus cells

Koyama¹,

Yamada²,

Matsuhashi³

1977

J Bacteriol

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“…A number of other N-acetylmuramyl-L-alanine amidase-producing bacteria have been shown to have this enzymic activity in maximal amounts during exponential growth. These include S. aureus Copenhagen (19), S. aureus Oeding 8507 (10,11), Bacillus subtilis 168 (23), and B. cereus T (H. J. Singer and B. D. Church, Bacteriol.…”

Section: Resultsmentioning

confidence: 99%

“…These include a glycosidase from strain 524 (6), an amidase from Oeding 8507 (11), a glucosaminidase from strain M19 (21) which also has amidase and peptidase activities in crude preparations, an amidase and glycine endopeptidase from strain EP-Kl (15), and an amidase, glucosaminidase, and glycine endopeptidase in strain K-6-W1 (1). The Oeding 8507 strain has also been shown to contain an amidase in both the cell wall and the soluble fraction (11). A wall-associated amidase has also been reported in strain Copenhagen (19).…”

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confidence: 99%

Properties and Purification ofN-Acetylmuramyl-l-Alanine Amidase fromStaphylococcus aureusH

1972

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The principal autolytic enzyme activity of the cell sap of Staphylococcus aureus H has been purified 400-fold. It is an N -acetylmuramyl- l -alanine amidase. This enzyme has a molecular weight of 8 to 10 × 10 5 , a p H optimum of 7.3, an ionic strength optimum of 0.16 m and a K m of 10 −3 m murein repeating units.

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“…They indicated that the primary defect of autolysin in RUS3 was in the amidase. S. aureus produce several extracellular autolysins including amidases and glucosaminidases, some of which have been purified to homogeneity while their physiological functions remain largely unknown (5,11,13,15,16).…”

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Isolation and Characterization of Staphylococcus aureus Mutants Which Form Altered Cell Clusters

Sugai

Komatsuzawa

Ooku-Inomata

et al. 1994

Microbiology and Immunology

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Staphylococcus aureus FDA 209P produces two extracellular bacteriolytic enzymes, 51‐kDa endo‐β‐N‐acetylglucosaminidase (GL) and 62‐kDa N‐acetylmuramyl‐l‐alanine amidase (AM), both of which can disperse cell clusters. To characterize the physiological roles of these enzymes in vivo, mutants with altered autolysin activity were isolated, and their degree of cluster formation in broth culture was assessed. Bacteriolytic activities of GL and AM, produced and secreted from these mutants into the culture fluid and detected with activity gels, coincided well with the degree of cluster formation of the mutants. The mutants with little or no enzyme activity grew in clusters, whereas those with high activity grew as well‐separated cocci, suggesting that these enzymes are involved in cell separation of S. aureus in vivo.

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Extracellular Cell Wall Lytic Enzyme from Staphylococcus aureus: Purification and Partial Characterization

Cited by 30 publications

References 19 publications

Formation of regular packets of Staphylococcus aureus cells

Formation of regular packets of Staphylococcus aureus cells

Properties and Purification ofN-Acetylmuramyl-l-Alanine Amidase fromStaphylococcus aureusH

Isolation and Characterization of Staphylococcus aureus Mutants Which Form Altered Cell Clusters

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