Extracellular biosynthesis of silver nanoparticles using Rhizopus stolonifer

Abstract

Synthesis of silver nanoparticles (AgNPs) has become a necessary field of applied science. Biological method for synthesis of AgNPs by aqueous mycelial extract was used. The AgNPs were identified by UV-visible spectrometry, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FT-IR). The presence of surface plasmon band around 420 nm indicates AgNPs formation. The characteristic of the AgNPs within the face-centered cubic (fcc) structure are indicated by the peaks of the X-ray diffraction (XRD) pattern corresponding to (1 1 1), (2 0 0) and (2 2 0) planes. Spherical, mono-dispersed and stable AgNPs with diameter around 9.47 nm were prepared and affirmed by high-resolution transmission electron microscopy (HR-TEM). Fourier Transform Infrared (FTIR) shows peaks at 1426 and 1684 cm that affirm the presence of coat covering protein the AgNPs which is known as capping proteins. Parameter optimization showed the smallest size of AgNPs (2.86 ± 0.3 nm) was obtained with 10 M AgNO at 40 °C. The present study provides the proof that the molecules within aqueous mycelial extract of facilitate synthesis of AgNPs and highlight on value-added from for cost effectiveness. Also, eco-friendly medical and nanotechnology-based industries could also be provided. Size of prepared AgNPs could be controlled by temperature and AgNO concentration. Further studies are required to study effect of more parameters on size and morphology of AgNPs as this will help in the control of large scale production of biogenic AgNPs.

Optimization of the biological synthesis of silver nanoparticles using Penicillium oxalicum GRS-1 and their antimicrobial effects against common food-borne pathogens

“…1 and analyzed for nitrate reductase activity. This was then mixed with AgNO 3 solution at a final concentration of 1 mm incubated on a rotary shaker (150 rpm, 28°C) for 72 h and cell filtrate without AgNO 3 was used as a control [21]. The transformations of the Ag ions into Ag nanoparticles were monitored by the UVvisible spectrophotometer and the nanoparticles were collected by ultracentrifugation (Hitachi Himac, CS 120GX, Japan) at 100,000 g for 30 min, purified by giving washings in alcohol and stored for further characterization.…”

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

“…The synthesis of nanoparticles (one billionth part of a meter) (10 -9 ) is the most important part of nanotechnology [1]. Nanomaterials can be synthesized with various biological, chemical, and physical methods [2].…”

Application of silver nanoparticles in food packages: a review

“…The introduction of nanotechnology in the food packaging industry may offer potential solutions for the challenge presented by short shelf life products, improving their quality and keeping them free of microbial adhesion (Emamifar et al, 2012;Qian et al, 2013). Metallic nanoparticles based on magnesium oxide, copper oxide, zinc oxide, cadmium selenite/tellurite, and titanium, silver and gold dioxide, have been studied because of their antimicrobial activity (AbdelRahim et al, 2017;Almeida et al, 2015;Echegoyen & Nerín, 2013;Silvestre et al, 2011).…”

“…Nowadays, research on biological synthesis of NPs has increased markedly, with emphasis in the microbial production of these compounds, as it is considered the most reliable and ecologically correct method (Wei et al, 2012). Among metallic nanoparticles, silver nanoparticles (AgNPs) have been widely studied due to their peculiar properties and their extensive application in the production of biomaterials (AbdelRahim et al, 2017), and in the food, cosmetics, clothing, and pharmaceutical industries (Chen et al, 2016;Kanmani & Lim, 2013). Additionally, when compared with other metals, silver presents the lowest toxicity for animal cells (Berni et al, 2008).…”