Extracellular biosynthesis of silver nanoparticle using Azadirachta indica leaf extract and its anti-microbial activity

Nazeruddin, G.M.; Prasad, N. N.; Waghmare, Shailesh R.; Mulla, I.S.

doi:10.1016/j.jallcom.2013.07.111

Cited by 101 publications

(56 citation statements)

References 39 publications

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“…Tannic acid [18], grape pomace extracts [1], Azadirachta indica leaf extract [13] and wheat bran xylan [4] have served as both reducing and capping agents to prepare silver nanoparticles. The silver nanoparticles so prepared tend to vary widely in shapes and sizes with coefficients of variation greater than 0.3, attributed to the structural and chemical complexity of these natural compounds [2,15,17,18].…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Hsieh

2016

International Journal of Biological Macromolecules

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a b s t r a c tSilver nanoparticles (AgNPs) were robustly synthesized from aqueous AgNO 3 with alkali lignin (low sulfonate) (AL ls ) severing as dual reducing and capping agent. The AgNP synthesis mechanisms were highly pH dependent. Under neutral and acidic conditions, polydispersed AgNPs were synthesized via the self-catalyzed reduction of Ag + on instantaneously formed Ag 2 O surfaces followed by the slower pseudo-first order reduction. The Ag 2 O nanoparticles functioned as the nucleating sites for the reduction of remaining silver cations to form AgNPs whose size and size distribution strongly dependent of lignin concentrations. AgNPs were optimally synthesized by reducing 2 mmol/L AgNO 3 with 0.16 wt% AL ls at pH 10 and 85• C in 30 min to near 100% yield in bimodal distributed sizes with 23% and 77% in feret diameters of 7.3 (±2.2) nm and 14.3 (±1.8) nm, respectively.

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Section: Introductionmentioning

confidence: 99%

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Hsieh

2016

International Journal of Biological Macromolecules

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“…This might be due to the young mass of cells with different ploidy levels in the callus, which may be metabolically active to produce various types of phytochemicals responsible for the bioreduction of metal atoms. 8,14 Additionally, TDZ is an important PGR, which had been reported for the production of important bioactive compounds in many plant species. 20,21 The variation in the production of these phytochemicals in response to different concentrations of TDZ can be due to the activation or the inhibition of key enzymes phenylalanine ammonia lyase and tyrosine ammonia lyase, which are crucial for biosynthetic pathways of these phytochemicals.…”

Section: Phytochemical Analysismentioning

confidence: 99%

“…Broad-spectrum bioactivities of AgNPs make them promising agents not only in fighting infections but also in tackling serious issues of tumors and, particularly, multidrug-resistant cancer cells. 8 Plant extracts are believed to act as reducing and capping agents in the synthesis of nanoparticle due to the presence of bioactive compounds. The nature of plant extract affects the kind of nanoparticles synthesized in a highly critical manner, with the source of plant extract being the most vital factor affecting the morphology of synthesized nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.

Anjum¹,

Abbasi²

2016

IJN

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Green synthesis of silver nanoparticles (AgNPs) by using plants is an emerging class of nanobiotechnology. It revolutionizes all domains of medical sciences by synthesizing chemical-free AgNPs for various biomedical applications. In this report, AgNPs were successfully synthesized by using whole plant extract (WPE) and thidiazuron-induced callus extract (CE) of Linum usitatissimum . The phytochemical analysis revealed that the total phenolic and flavonoid contents were higher in CE than that in WPE. Ultraviolet-visible spectroscopy of synthesized AgNPs showed a characteristic surface plasmon band in the range of 410–426 nm. Bioreduction of CE-mediated AgNPs was completed in a shorter time than that of WPE-mediated AgNPs. Scanning electron microscopy showed that both types of synthesized AgNPs were spherical in shape, but CE-mediated AgNPs were smaller in size (19–24 nm) and more scattered in distribution than that of WPE-mediated AgNPs (49–54 nm). X-ray diffraction analysis confirmed crystalline nature (face-centered cubic) of both types of AgNPs. Fourier-transform infrared spectroscopy revealed that the polyphenols and flavonoids were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further confirmed the successful synthesis of AgNPs. Moreover, the synthesized AgNPs were found to be stable over months with no change in the surface plasmon bands. More importantly, CE-mediated AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens than WPE-mediated AgNPs. The present work highlighted the potent role of thidiazuron in in vitro-derived cultures for enhanced biosynthesis of chemical-free AgNPs, which can be used as nanomedicines in many biomedical applications.

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“…A large numbers of secondary metabolites of plants are used in food additives, fragrances, pigments or in medicines. Flavonoids are used as important neutraceuticals and pharmaceuticals [10][11][12] . This article is concerned with the synthesis of silver nanoparticles by using Piper nigrum seeds' water extract.…”

Section: Introductionmentioning

confidence: 99%

Water Extract of Piper Nigrum Seed Assisted Synthesis of Silver Nanoparticles and Reduction of Various Nitro Compounds by NaBH4 in the Presence of AgNPs as a Catalyst

2018

Chem Sci Trans

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An attempt has been made for eco-friendly synthesis of silver nanoparticles by water extract of Piper nigrum seed. Formation of AgNPs and their morphology was confirmed by using different advanced characterization techniques such as UV-Visible spectroscopy, XRD and FE-SEM images of AgNPs. Further we wish to report for the first time reduction of various nitro compounds to corresponding amines by NaBH 4 in the presence of AgNPs as a catalyst. After reaction course, AgNPs can be recycled and reused without any apparent loss of activity which makes this process cost effective and hence eco-friendly. The structures of all the corresponding reduced products were confirmed by comparing their IR, 1 H NMR, spectra with the authentic samples.

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Extracellular biosynthesis of silver nanoparticle using Azadirachta indica leaf extract and its anti-microbial activity

Cited by 101 publications

References 39 publications

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.

Water Extract of Piper Nigrum Seed Assisted Synthesis of Silver Nanoparticles and Reduction of Various Nitro Compounds by NaBH4 in the Presence of AgNPs as a Catalyst

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