Extracellular ATPs produced in seminal plasma exosomes regulate boar sperm motility and mitochondrial metabolism

Guo, Huijuan; Chang, Zhanglin; Zhao, Yuting; Jiang, Xueyuan; Yu, Hongyan; Zhang, Yanwen; Zhao, Ruqian

doi:10.1016/j.theriogenology.2019.08.003

Cited by 27 publications

(15 citation statements)

References 37 publications

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“…Interestingly, pOF produced a highest chromatin decondensation in ejaculated spermatozoa than in the epididymal ones. Probably the SP provides some components, as exosomes 53,66,67 , that Percoll did not remove, and that facilitate the action of pOF decondensing the sperm chromatin. However, this hypothesis still needs to be demonstrated.…”

Section: Discussionmentioning

confidence: 99%

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Epididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs

et al. 2020

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Background The current results of in vitro reproduction techniques in pigs, such as in vitro fertilization (IVF) and embryo development, show high performance with both epididymal and ejaculated spermatozoa. However, the results using ejaculated spermatozoa are even better. Ejaculated spermatozoa are exposed to the secretions of the accessory seminal glands: the seminal plasma (SP). It has been reported that exposure of spermatozoa to reproductive fluids, such as SP or periovulatory oviductal fluid (pOF), modulates sperm functionality both in vivo and in vitro. But whether or not this modulating effect of pOF depends on the origin of the spermatozoa being epididymal or ejaculated, is still unknown. Objectives To determine and compare the effect of pOF on epididymal and ejaculated sperm functionality. Material and methods The effects of incubating spermatozoa from the epididymis and ejaculate with pOF in capacitating conditions were investigated by analyzing sperm motility, phosphorylation of protein kinase A substrates and proteins in tyrosine (pPKAs and pTyr, respectively), the interaction of the spermatozoa with the oocyte in IVF and intracytoplasmic sperm injection (ICSI), and, finally, the spermatozoa chromatin condensation status. Results The pOF modified events related to capacitation in epididymal spermatozoa by decreasing motility, pPKAs and pTyr. In the interaction with the oocyte after sperm capacitation, pOF regulated the epididymal and ejaculated spermatozoa differently. While pOF decreased the number of spermatozoa bound to the zona pellucida (Spz/ZP) and increased oocyte activation after ICSI with epididymal spermatozoa, with the ejaculated spermatozoa, it decreased the mean number penetrating each oocyte (Spz/O). Additionally, pOF significantly increased the nuclear decondensation of the epididymal spermatozoa after the fertilization of the oocyte. Conclusion The modulation of sperm functionality by pOF is conditioned by the origin of the spermatozoa.

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Section: Discussionmentioning

confidence: 99%

“…Probably the SP provides some components, as exosomes 53,66,67 , that Percoll did not remove, and that facilitate the action of pOF decondensing the sperm chromatin. However, this hypothesis still needs to be demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Epididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs

et al. 2020

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“…One of these showed that ATP can be also produced in exosomes derived from seminal plasma and it normally assures a safe environment for sperm viability and fertilization in mammals. For instance, such ATP regulates boar sperm motility [35]. In agreement with these data, a recent review collected findings on the possibility that EVs are able to support surrounding cells by glycolytic formation of ATP [36].…”

Section: Purines and Evs Derived From Normal Or Pathological Non-cancer Cells/tissuesmentioning

confidence: 71%

Adenine-Based Purines and Related Metabolizing Enzymes: Evidence for Their Impact on Tumor Extracellular Vesicle Activities

Iorio

Ciccarelli

2021

Cells

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Extracellular vesicles (EVs), mainly classified as small and large EVs according to their size/origin, contribute as multi-signal messengers to intercellular communications in normal/pathological conditions. EVs are now recognized as critical players in cancer processes by promoting transformation, growth, invasion, and drug-resistance of tumor cells thanks to the release of molecules contained inside them (i.e., nucleic acids, lipids and proteins) into the tumor microenvironment (TME). Interestingly, secretion from donor cells and/or uptake of EVs/their content by recipient cells are regulated by extracellular signals present in TME. Among those able to modulate the EV-tumor crosstalk, purines, mainly the adenine-based ones, could be included. Indeed, TME is characterized by high levels of ATP/adenosine and by the presence of enzymes deputed to their turnover. Moreover, ATP/adenosine, interacting with their own receptors, can affect both host and tumor responses. However, studies on whether/how the purinergic system behaves as a modulator of EV biogenesis, release and functions in cancer are still poor. Thus, this review is aimed at collecting data so far obtained to stimulate further research in this regard. Hopefully, new findings on the impact of adenine purines/related enzymes on EV functions may be exploited in tumor management uncovering novel tumor biomarkers and/or druggable targets.

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“…Related studies have proven that exosomes can affect semen quality and enter sperm, thereby enhancing sperm motor ability and capacitation after ejaculation[ 183 ]. Studies have shown that seminal plasma exosomes regulate sperm motility and mitochondrial metabolism in mammals[ 184 ]. The epididymis plays an important role in sperm maturation in the male reproductive tract, and male sperm rely heavily on interactions with epididymal epithelial secretions[ 185 ].…”

Section: Role Of Stem Cell-derived Extracellular Vesicles In Male Fermentioning

confidence: 99%

Stem cell-based therapies for fertility preservation in males: Current status and future prospects

Liu¹,

Xie²,

Deng³

et al. 2020

WJSC

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With the decline in male fertility in recent years, strategies for male fertility preservation have received increasing attention. In this study, by reviewing current treatments and recent publications, we describe research progress in and the future directions of stem cell-based therapies for male fertility preservation, focusing on the use of spermatogonial stem cells (SSCs), SSC niches, SSC-based testicular organoids, other stem cell types such as mesenchymal stem cells, and stem cell-derived extracellular vesicles. In conclusion, a more comprehensive understanding of the germ cell microenvironment, stem cell-derived extracellular vesicles, and testicular organoids will play an important role in achieving male fertility preservation.

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Extracellular ATPs produced in seminal plasma exosomes regulate boar sperm motility and mitochondrial metabolism

Cited by 27 publications

References 37 publications

Epididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs

Epididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs

Adenine-Based Purines and Related Metabolizing Enzymes: Evidence for Their Impact on Tumor Extracellular Vesicle Activities

Stem cell-based therapies for fertility preservation in males: Current status and future prospects

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