Extracellular ATP, ecto‐ATPase and calcium influx in Dictyostelium discoideum cells

Parish, Roger W.; Weibel, Monika

doi:10.1016/0014-5793(80)80234-1

Cited by 45 publications

(20 citation statements)

References 17 publications

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“…In addition, endostatin could hydrolyze ADP, but not AMP ( Fig. 2A), showing that endostatin can cleave both b-g and a-b phosphodiester bonds, similar to apyrase and ecto-ATPase families (35,36). Concordant with these results, endostatin hydrolyzed ATP-g-S at a similar rate relative to ADP.…”

Section: Endostatin Has Novel Atpase Activitysupporting

confidence: 73%

Endostatin Has ATPase Activity, Which Mediates Its Antiangiogenic and Antitumor Activities

Wang

Liu

et al. 2015

Molecular Cancer Therapeutics

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Endostatin is an endogenous angiogenesis inhibitor with broad-spectrum antitumor activities. Although the molecular mechanisms of endostatin have been extensively explored, the intrinsic biochemical characteristics of endostatin are not completely understood. Here, we revealed for the first time that endostatin embedded novel ATPase activity. Moreover, mutagenesis study showed that the ATPase activity of endostatin mutants positively correlated with effects on endothelial cell activities and tumor growth. E-M, an endostatin mutant with higher ATPase activity than that of wild-type (WT) endostatin, significantly increased endostatin-mediated inhibitory effects on endothelial cell proliferation, migration, tube formation, and adhesion. In vivo study showed that E-M displayed enhanced antitumor effects compared with WT. On the other hand, K96A, K96R, and E176A, endostatin mutants with lower ATPase activities than that of WT, showed reduced or comparable effects on targeting both in vitro endothelial cell activities and in vivo tumor angiogenesis and tumor growth. Furthermore, endostatin and its mutants exhibited distinct abilities in regulations of gene expression (Id1, Id3), cell signaling (Erk, p38, and Src phosphorylation), and intracellular ATP levels. Collectively, our study demonstrates that endostatin has novel ATPase activity, which mediates its antiangiogenic and antitumor activities, suggesting that construction of endostatin analogues with high ATPase activity may provide a new direction for the development of more potent antiangiogenic drugs.

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Section: Endostatin Has Novel Atpase Activitysupporting

confidence: 73%

Endostatin Has ATPase Activity, Which Mediates Its Antiangiogenic and Antitumor Activities

Wang

Liu

et al. 2015

Molecular Cancer Therapeutics

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“…It has been clear for some time that Dictyostelium secrete ATP in culture and posses membrane-bound enzymes capable of degrading ATP to ADP and adenosine (Parish and Weibel 1980;Hodge and Rossomando 1980). It is therefore not surprising that Dictyostelium also has plasma membrane receptors for ATP (Ludlow et al 2008).…”

Section: Purinergic Receptorsmentioning

confidence: 97%

Neurotransmitter Receptor Homologues of Dictyostelium discoideum

Fountain

2009

J Mol Neurosci

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The social amoeba Dictyostelium discoideum is a genetically amenable eukaryotic cell which displays many animal cell traits and has been used to study cellular signalling for over 30 years. Recently studies have highlighted the roles that molecules associated with synaptic transmission in animals, such as glutamate, GABA and ATP play in cellular differentiation and homeostasis in this simple organism. This short review summarises the evidence for the existence of both ionotropic and metabotropic families of neurotransmitter receptors in Dictyostelium.

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“…In the last decades, it is found that animal, plant, and microbial cells can secrete ATP from the cytosol into the extracellular matrix (Parish and Weibel 1980, Boyum and Guidotti 1997, Thomas et al 2000.…”

Section: Introductionmentioning

confidence: 99%

Extracellular ATP affects chlorophyll fluorescence of kidney bean (Phaseolus vulgaris) leaves through Ca2+ and H2O2-dependent mechanism

Feng¹,

Jiao²,

Sun³

et al. 2015

Photosynt.

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Extracellular ATP (eATP) has been considered as an important extracellular compound to mediate several physiological processes in plant cells. We investigated the effects of eATP on chlorophyll (Chl) fluorescence characteristics of kidney bean (Phaseolus vulgaris) leaves. Treatment with exogenous ATP at 1 mM showed no significant effect on the maximal photochemical efficiency of PSII. However, the treatment significantly enhanced the values of the PSII operating efficiency (Φ PSII ), rate of photosynthetic electron transport through PSII (ETR), and photochemical quenching (q P ), while the values of the nonphotochemical quenching (q N ) and quantum yield of regulated energy dissipation of PSII (Y NPQ ) significantly decreased. Our observations indicated that eATP stimulated the PSII photochemistry in kidney bean leaves. Similarly, the treatment with exogenous Ca 2+ or H 2 O 2 at 1 mM caused also the significant increase in Φ PSII , q P , and ETR and the significant decrease in q N and Y NPQ . LaCl 3 (an inhibitor of Ca 2+ channels) and dimethylthiourea (a scavenger of H 2 O 2 ) abolished the effects of exogenous ATP. The results suggest that the role of eATP in enhancing the PSII photochemistry could be related to a Ca 2+ or H 2 O 2 signaling pathway.

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Extracellular ATP, ecto‐ATPase and calcium influx in Dictyostelium discoideum cells

Cited by 45 publications

References 17 publications

Endostatin Has ATPase Activity, Which Mediates Its Antiangiogenic and Antitumor Activities

Endostatin Has ATPase Activity, Which Mediates Its Antiangiogenic and Antitumor Activities

Neurotransmitter Receptor Homologues of Dictyostelium discoideum

Extracellular ATP affects chlorophyll fluorescence of kidney bean (Phaseolus vulgaris) leaves through Ca<sup>2+</sup> and H<sub>2</sub>O<sub>2</sub>-dependent mechanism

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