2000
DOI: 10.1038/sj.bjp.0703563
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Extracellular ATP‐dependent activation of plasma membrane Ca2+ pump in HEK‐293 cells

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Cited by 19 publications
(13 citation statements)
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“…Simultaneous activation of the G protein-coupled receptor signaling pathway by carbachol and inhibition of either CaM by calmidazolium or CaMKII by KN-62 had additive effects on ASP ϩ uptake, indicating the independence of the carbachol-mediated inhibition and the CaM-mediated stimulation of hOCT2 transport. Carbachol or ATP increased [Ca 2ϩ ] i in this and previous reports from HEK-293 cells (27,37). This increase in [Ca 2ϩ ] i should have increased CaM activity and, therefore, stimulated hOCT2 transport; however, the opposite was true.…”
Section: Discussioncontrasting
confidence: 42%
See 1 more Smart Citation
“…Simultaneous activation of the G protein-coupled receptor signaling pathway by carbachol and inhibition of either CaM by calmidazolium or CaMKII by KN-62 had additive effects on ASP ϩ uptake, indicating the independence of the carbachol-mediated inhibition and the CaM-mediated stimulation of hOCT2 transport. Carbachol or ATP increased [Ca 2ϩ ] i in this and previous reports from HEK-293 cells (27,37). This increase in [Ca 2ϩ ] i should have increased CaM activity and, therefore, stimulated hOCT2 transport; however, the opposite was true.…”
Section: Discussioncontrasting
confidence: 42%
“…To investigate the effect of activation of G protein-coupled receptor on ASP ϩ uptake in hOCT2-transfected HEK-293 cells, we used the purinergic agonist ATP and the muscarinic agonist carbachol. Receptors for both agonists have been demonstrated in HEK-293 cells (27,37). ATP and carbachol resulted in inhibition of ASP ϩ uptake by ϳ34 and 44%, respectively.…”
Section: Discussionmentioning
confidence: 99%
“…2C). This increase in calcium by P2Y receptors upon BzATP application has been previously reported for native HEK-293 cells (23). The addition of 50 M BzATP to HEK-293 cells transfected with the full-length rat P2X7R caused a sustained increase in fluo-4 fluorescence, which was interpreted as an influx of calcium through the P2X7 channel (Fig.…”
Section: Functional Analysis Of Pore Formation and Channel Activity Imentioning
confidence: 87%
“…4D). ATP and other agonists have been reported previously to activate the PMCA in HEK293 cells (Qi et al, 2000) and other cell types (Usachev et al, 2002).…”
Section: Resultsmentioning
confidence: 99%
“…2A. The actions of a different PMCA inhibitor, sodium orthovanadate (Qi et al, 2000), are shown in Fig. 2E.…”
Section: Resultsmentioning
confidence: 99%