Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization

Patel, Rajesh; Dodia, M. S.; Singh, Satya P.

doi:10.1016/j.procbio.2005.03.049

Cited by 171 publications

(109 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The present results demonstrated that an increased supply of yeast extract as well as peptone stimulated the expression of alkaline protease by the Bacillus strain NA7. These positive effects of the two protein-rich complexes on growth and protease synthesis by Bacillus cells match previous findings (Kaur et al, 2001;Patel et al, 2005;Anbu, 2013). Though the presence of casein enhances protease production in some Bacillus strains (Belmessikh et al, 2013), it is likely that casein is not among the best protease inducers for the experimental Bacillus strain.…”

Section: Discussionsupporting

confidence: 88%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated strain produced thermostable extracellular alkaline protease with relatively stable maximum activity records (0.610-0.625 TU) within a relatively long stationary phase that exceeded 60 h. A 2-level fractional factorial design (Plackett-Burman) was then applied to screen for nutritional and cultivation factors regulating protease production by the isolate and to appraise their effects. Calculated statistical parameters revealed that NaCl and MgSO 4 are the most significant independent variables affecting alkaline protease production by NA7 and suggested a near-optimum culture condition. Verification of this predicted condition resulted in an alkaline protease specific activity record of 509 TU/mg protein with a 1.27 fold increase when compared to the basal medium culture.

show abstract

Section: Discussionsupporting

confidence: 88%

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Lotfy¹,

Abd-El-Karim²,

El-Sharouny³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Recently, a newly isolated haloalkaliphilic Bacillus sp. was reported to produce optimum level of alkaline protease in gelatin broth (29).…”

Section: Resultsmentioning

confidence: 99%

Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1

Thumar

Singh

2007

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

An alkaliphilic and salt-tolerant actinomycete, Streptomyces clavuligerus strain Mit-1, was isolated from Mithapur, the western coast of India. The organism was Gram-positive, having filamentous, long thread like structure. The sporulation started after two days of growth and the optimum level of alkaline protease (130 U/ ml) was produced during the early stationary phase. The strain could grow and produce protease with 0-10% NaCl (w/v), the optimum being 5% NaCl (w/v). Growth and protease production was optimum at pH 9 with substantial decline at neutral pH. Sucrose and gelatin were the best carbon and nitrogen sources respectively, whereas gelatin broth was the preferred medium for protease production. Mit-1 produced substantial protease with various amino acids, when employed as the sole nitrogen sources. Crude substrates, such as molasses, whey and wheat flour had significant effect on enzyme production. The results are quite valuable, as only few actinomycetes, particularly salt-tolerant alkaliphilic ones, have so far been explored for their enzymatic potential and process optimization.

show abstract

“…L21 (306.5 U/ml) (Tari et al, 2006), Bacillus sp. (410 U/ml) (Patel et al, 2005), B. mojavensis (558 U/ml) (Beg et al, 2003).…”

Section: Central Composite Designmentioning

confidence: 99%

Response surface method to optimize the low cost medium for protease production using anchovy meal from ascidian associated Bacillus sp. GA CAS10

Kumar¹,

Ananthan²,

Iyappan³

2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

A protease producing Bacillus sp. GA CAS10 was isolated from ascidian Phallusia arabica, Tuticorin, Southeast coast of India. Response surface methodology was employed for the optimization of different nutritional and physical factors for the production of protease. Plackett-Burman method was applied to identify important factors (anchovy waste, KH 2 PO 4 , NaCl and temperature) influencing protease production. Further optimization was done by response surface methodology using central composite design. Under the optimized conditions by central composite design, the protease experimental yield (842.102 U/ml) closely matched the predicted yield by the statistical model (830.307 U/ml) with R 2 = 99.94%. The time course of protease production was increased using the RSM optimized medium (856.29 U/ml) (anchovy waste 20.50 g/l, KH 2 PO 4 3.06 g/l, NaCl 42.91 g/l, temperature 43.36°C, 42 h and pH 9) compared with the un-optimized basal medium (267.33 U/ml). The improvement of protease production by microbial conversion of anchovy waste suggested its potential utilization to generate high value added products using cheap carbon and nitrogen substrates.

show abstract

Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization

Cited by 171 publications

References 38 publications

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1

Response surface method to optimize the low cost medium for protease production using anchovy meal from ascidian associated Bacillus sp. GA CAS10

Contact Info

Product

Resources

About