Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation and exocytosis in heterotroph cell cultures of Chenopodium rubrum

Chaidee, Anchalee; Wongchai, Chatchawal; Pfeiffer, Wolfgang

doi:10.1016/j.jplph.2007.12.012

Cited by 5 publications

(2 citation statements)

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“…Few natural insecticides such as pyrethroids stop phosphatase (Ishaaya, I. and Casida, 1980) [47]. And elevated levels of lactic dehydrogenase also create oxygen deficient stage and cause tissue necrosis.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Tagetes erecta Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite Odontotermes obesus

Kumar¹,

Upadhyay²

2023

AER

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Plant essential oils and their constituents have proven to be very effective against insects, especially termites. They are the best alternative to synthetic pesticides that are harmless to the environment and human health. In the present study, different enzymes, namely alkaline phosphatase, acid phosphatase, glutamate-oxaloacetate transaminase, glutamate-pyruvate transaminase and acetylcholinesterase, were evaluated in Indian termite Odontotermes obesus in a combination preparation based on Tagetes erecta essential oil. For the study of anti-termite effects against worker termites were treated topically with 40% and 80% of the 24-hour LD 50 values of various combination formulations. Subsequent bioassays at 40% and 80% of LD 50 of combinatorial preparations S-AST-A, S-AST-B, and S-AST-C resulted in a significant (p > 0.05) decrease in glutamate-oxaloacetate transaminase. After 16 hours of treatment, they were 87.92%, 80.17%, 89.30%, 79.17%, 81.92% and 73.17% respectively. In vivo exposures of 40% and 80% of the LD 50 of the combination formulation caused a highly significant (p > 0.05) reduction (p > 0.05) of all test enzymes tested compared to controls. The effects of different oil formulations exhibited time-and dose-dependent responses, resulting in physiological changes in the concentrations of various enzymes. The combined mixture of Tagetes erecta essential oils has significantly better anti-termite ability compared to inorganic insecticides. Findings from this study will help support termite control in fields, gardens and homes in a sustainable way, without the downsides of insecticide resistance and pollution. These could potentially be used to produce commercial formulations for use against pests.

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Section: Discussionmentioning

confidence: 99%

Toxicity of Tagetes erecta Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite Odontotermes obesus

Kumar¹,

Upadhyay²

2023

AER

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“…Heterotroph and autotroph suspension cells of Chenopodium rubrum L. have been established as a model for membrane biology (Kranewitter et al. 1999; Pfeiffer & Hoeftberger 2000, 2001), and abiotic stress sensing showing heat shock responses of the extracellular pH, proteins and cytoskeleton in heterotroph and autotroph cells of this species (Chaidee & Pfeiffer 2006; Chaidee et al. 2008a,b).…”

Section: Introductionmentioning

confidence: 99%

Multivariate analyses of salt stress and metabolite sensing in auto‐ and heterotroph Chenopodium cell suspensions

2011

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Global warming increases plant salt stress via evaporation after irrigation, but how plant cells sense salt stress remains unknown. Here, we searched for correlation-based targets of salt stress sensing in Chenopodium rubrum cell suspension cultures. We proposed a linkage between the sensing of salt stress and the sensing of distinct metabolites. Consequently, we analysed various extracellular pH signals in autotroph and heterotroph cell suspensions. Our search included signals after 52 treatments: salt and osmotic stress, ion channel inhibitors (amiloride, quinidine), salt-sensing modulators (proline), amino acids, carboxylic acids and regulators (salicylic acid, 2,4-dichlorphenoxyacetic acid). Multivariate analyses revealed hirarchical clusters of signals and five principal components of extracellular proton flux. The principal component correlated with salt stress was an antagonism of γ-aminobutyric and salicylic acid, confirming involvement of acid-sensing ion channels (ASICs) in salt stress sensing. Proline, short non-substituted mono-carboxylic acids (C2-C6), lactic acid and amiloride characterised the four uncorrelated principal components of proton flux. The proline-associated principal component included an antagonism of 2,4-dichlorphenoxyacetic acid and a set of amino acids (hydrophobic, polar, acidic, basic). The five principal components captured 100% of variance of extracellular proton flux. Thus, a bias-free, functional high-throughput screening was established to extract new clusters of response elements and potential signalling pathways, and to serve as a core for quantitative meta-analysis in plant biology. The eigenvectors reorient research, associating proline with development instead of salt stress, and the proof of existence of multiple components of proton flux can help to resolve controversy about the acid growth theory.

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Enhancement and quantification of repellent activity in Chenopodium cells

Wongchai

Chaidee

Pfeiffer

2012

Arthropod-Plant Interactions

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Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation and exocytosis in heterotroph cell cultures of Chenopodium rubrum

Cited by 5 publications

References 40 publications

Toxicity of <i>Tagetes erecta</i> Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite <i>Odontotermes obesus</i>

Toxicity of <i>Tagetes erecta</i> Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite <i>Odontotermes obesus</i>

Multivariate analyses of salt stress and metabolite sensing in auto‐ and heterotroph Chenopodium cell suspensions

Enhancement and quantification of repellent activity in Chenopodium cells

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Extracellular alkaline phosphatase is a sensitive marker for cellular stimulation and exocytosis in heterotroph cell cultures of Chenopodium rubrum

Cited by 5 publications

References 40 publications

Toxicity of &lt;i&gt;Tagetes erecta&lt;/i&gt; Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite &lt;i&gt;Odontotermes obesus&lt;/i&gt;

Toxicity of &lt;i&gt;Tagetes erecta&lt;/i&gt; Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite &lt;i&gt;Odontotermes obesus&lt;/i&gt;

Multivariate analyses of salt stress and metabolite sensing in auto‐ and heterotroph Chenopodium cell suspensions

Enhancement and quantification of repellent activity in Chenopodium cells

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Toxicity of <i>Tagetes erecta</i> Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite <i>Odontotermes obesus</i>

Toxicity of <i>Tagetes erecta</i> Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite <i>Odontotermes obesus</i>