EXTRACCIÓN DE COMPUESTOS FENÓLICOS DE LA CÁSCARA DE LIMA (Citrus limetta Risso) Y DETERMINACIÓN DE SU ACTIVIDAD ANTIOXIDANTE

Pérez-Nájera, Viridiana Candelaria; Lugo-Cervantes, EC; Gutiérrez-Lomelí, M; Del‐Toro‐Sánchez, Carmen Lizette

doi:10.18633/bt.v15i3.153

Cited by 15 publications

(10 citation statements)

References 19 publications

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“…A total of 8 methanolic plant extracts were tested against 5 clinical bacterial isolates. Methanol was selected as extraction solvent, because it is one of the best solvents used for the extraction of antimicrobial substances [ 29 , 30 ]. Moreover, methanol polarity ensured the extraction of polar and moderately polar active compounds from plants against microorganisms like terpenoids, tannins, flavones, and polyphenols [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms

Sánchez

Morales

Castillo

et al. 2016

Evidence-Based Complementary and Alternative Medicine

110

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Biofilm is a complex microbial community highly resistant to antimicrobials. The formation of biofilms in biotic and abiotic surfaces is associated with high rates of morbidity and mortality in hospitalized patients. New alternatives for controlling infections have been proposed focusing on the therapeutic properties of medicinal plants and their antimicrobial effects. In the present study the antimicrobial and antibiofilm activities of 8 methanolic plant extracts were evaluated against clinical isolated microorganisms. Preliminary screening by diffusion well assay showed the antimicrobial activity of Prosopis laevigata, Opuntia ficus-indica, and Gutierrezia microcephala. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined ranging from 0.7 to >15 mg/mL. The specific biofilm formation index (SBF) was evaluated before and after the addition of plant extracts (MBC × 0.75). Opuntia ficus-indica caused the major reduction on SBF in dose-dependent manner. Cytotoxic activity of plant extracts was determined using brine shrimp lethality test (Artemia salina L.). Lethal Dose concentration (LD50 values) of the plant extracts was calculated. LD50 values for P. laevigata and G. microcephala were 141.6 and 323.3 µg/mL, respectively, while O. ficus-indica showed a slight lethality with 939.2 µg/mL. Phytochemical analyses reveal the presence of flavonoids, tannins, and coumarines.

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Section: Resultsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms

Sánchez

Morales

Castillo

et al. 2016

Evidence-Based Complementary and Alternative Medicine

110

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“…In this study, chiltepin pepper showed different antioxidant activity behavior by each of the 3 methods, which can be associated to the different mechanism involved in each method. DPPH is an antioxidant capacity method that measures mainly lipophilic compounds, while the ABTS is associated with the antioxidant activity of hydrophilic compounds in alcoholic extracts (Pérez-Nájera et al, 2013). In addition, both methods base their results on the neutralization of a free radical, which is more representative of the behavior of oxidative stress at biological level.…”

Section: Discussionmentioning

confidence: 99%

A review of the evolution in the research of antioxidants in olives and olive oil during the last four decades

Kiritsakis¹,

Kiritsakis²,

Tsitsipas³

2020

JFB

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Quantification of chiltepin hot pepper (Capsicum annuum L. glabriusculum) phytochemicals provides a tool to evaluate the fruit quality and health impact. This study evaluates the phytochemical content and antioxidant activity of chiltepin from different locations of Sonora, Mexico, at two ripening stages (immature and mature). Seeds from Cumpas and Sahuaripa, were grown under greenhouse conditions and phenolic compounds, flavonoids, carotenoids, chlorophylls, and ascorbic acid were determined by spectrophotometric techniques. Capsaicinoids were determined by HPLC-DAD. The antioxidant activity was determined through DPPH and ABTS radical scavenging and by FRAP techniques. The origin of the seed influenced the antioxidant activity and phytochemical content. Samples from Cumpas, were superior in phytochemicals compared with Sahuaripa. Antioxidant activity and phytochemicals were higher in mature stage. Antioxidant activity correlates mainly with phenolic compounds and carotenoids. This study highlights that mature chiltepin pepper from Sonora could grow under controlled conditions develop bioactive compounds with antioxidant potential.

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“…It was also noted in Table 2, in most treatments FRAP values were approximately twice as those obtained through DPPH. Nevertheless, is possible that some antioxidant compounds, such as carotenoids and some sugars can interfere in the measurement of DPPH methodology (Pérez, Lugo, Gutiérrez & Del-Toro, 2013).…”

Section: Discussionmentioning

confidence: 99%

Microwave extraction of champa (Campomanesia lineatifolia Ruiz & Pav.) fruit: alternative to obtain natural antioxidants

Otálvaro-Álvarez¹,

PABÓN-BAQUERO²,

Rendón-Fernández³

et al. 2018

Acta Agron.

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Champa (Campomanesia lineatifolia Ruiz & Pav.) is a fruit tree that belongs to Myrtaceae family and is native from the Amazon. Champa fruit has special organoleptic characteristics like its flavor and is an important source of phenolic compounds useful in food industry. Currently, the postharvest process of champa fruit needs to be improved due to fruit losses, which had achieved a 97%. In this sense, the aim of this research was to evaluate microwave extraction to obtain extracts enriched in phenolic compounds from champa fruit. The evaluated variables were as follows: microwave power (100-200W), extraction time (1-2min) and solvent (water, ethanol-water 70:30 and ethanol-water 96:4), respectively. Results showed that the best condition to obtain phenolic extracts involved the use of water as solvent, 100W of power and 2min of extraction time. Given these concerns, was possible to obtain a yield higher than 60%. The obtained extracts had achieved a maximum antioxidant activity of 11.36 and 31.44 mg of Trolox.g -1 of dry pulp through DPPH and FRAP methods. In fact, the higher phenolic concentration (3450.73 μg Gallic acid. g -1 of dry pulp) was obtained with a 200 W of microwave power, water as solvent and 1 min of extraction time. These results suggest that champa fruit is a source of phenolic extracts with antioxidant activity, which are useful for agroindustry sector, especially as natural additives to extend the shelf life of different products.Key words: Agroindustrial biotechnology, food processing, fruit extracts, natural additives, phenolic compounds, fruit postharvest. ResumenLa Champa (Campomanesia lineatifolia Ruiz & Pav.) es un árbol frutal nativo del amazonas perteneciente a la familia Myrtaceae. La fruta de la champa tiene unas características organolépticas especiales como su sabor y es una importante fuente de compuestos fenólicos útiles para la industria de los alimentos. Actualmente los procesos poscosecha de la fruta requieren mejoras pues las pérdidas alcanzan el 97%. En ese sentido, el objetivo de esta investigación fue evaluar la extracción por microondas de la champa para la obtención de extractos enriquecidos en compuestos fenólicos. Las variables estudiadas fueron: potencia del microondas (100-200W), tiempo de extracción (1-2min) y disolvente (agua, mezcla etanol: agua 70:30 y mezcla etanol: agua 96:4). Los resultados mostraron que la mejor condición para la obtención de extractos fenólicos fue empleando agua como disolvente, una potencia de 100W y 2 min de tiempo de extracción. A esta condición, se alcanzó un rendimiento de extracción superior al 60%. La máxima actividad antioxidante en los extractos fue de 11.36 y 31.44 mg eq de Trolox.g -1 de pulpa seca, evaluada por los métodos del DPPH y FRAP respectivamente. La mayor concentración de contenido fenólico fue obtenida (3450.73 μg de ácido gálico.g -1 de pulpa seca) utilizando como condiciones de proceso una potencia del microondas de 200W, agua como disolvente y un tiempo de extracción de 1min. Estos resultados sugieren que la...

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EXTRACCIÓN DE COMPUESTOS FENÓLICOS DE LA CÁSCARA DE LIMA (Citrus limetta Risso) Y DETERMINACIÓN DE SU ACTIVIDAD ANTIOXIDANTE

Cited by 15 publications

References 19 publications

Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms

Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms

A review of the evolution in the research of antioxidants in olives and olive oil during the last four decades

Microwave extraction of champa (Campomanesia lineatifolia Ruiz & Pav.) fruit: alternative to obtain natural antioxidants

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