Extra virgin olive oil phenolic extracts counteract the pro-oxidant effect of dietary oxidized lipids in human intestinal cells

Incani, Alessandra; Serra, Gino; Atzeri, Angela; Melis, Maria Paola; Serreli, Gabriele; Bandino, G.; Sedda, P.; Campus, Marco; Tuberoso, Carlo Ignazio Giovanni; Deiana, Monica

doi:10.1016/j.fct.2016.02.015

Cited by 57 publications

(46 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is thus important to determine the composition of extra virgin olive oils (EVOO) used as the reference in the present study and to establish whether particular compounds of these oils have cytoprotective effects on relevant in vitro models [57]. Usually, EVOO are characterized by high levels of oleic acid and are rich in tocopherols, which exhibit significant antioxidant activities [58,59].…”

Section: Resultsmentioning

confidence: 99%

Protective Effects of α-Tocopherol, γ-Tocopherol and Oleic Acid, Three Compounds of Olive Oils, and No Effect of Trolox, on 7-Ketocholesterol-Induced Mitochondrial and Peroxisomal Dysfunction in Microglial BV-2 Cells

Debbabi

Nury

Zarrouk

et al. 2016

IJMS

View full text Add to dashboard Cite

Lipid peroxidation products, such as 7-ketocholesterol (7KC), may be increased in the body fluids and tissues of patients with neurodegenerative diseases and trigger microglial dysfunction involved in neurodegeneration. It is therefore important to identify synthetic and natural molecules able to impair the toxic effects of 7KC. We determined the impact of 7KC on murine microglial BV-2 cells, especially its ability to trigger mitochondrial and peroxisomal dysfunction, and evaluated the protective effects of α- and γ-tocopherol, Trolox, and oleic acid (OA). Multiple complementary chemical assays, flow cytometric and biochemical methods were used to evaluate the antioxidant and cytoprotective properties of these molecules. According to various complementary assays to estimate antioxidant activity, only α-, and γ-tocopherol, and Trolox had antioxidant properties. However, only α-tocopherol, γ-tocopherol and OA were able to impair 7KC-induced loss of mitochondrial transmembrane potential, which is associated with increased permeability to propidium iodide, an indicator of cell death. In addition, α-and γ-tocopherol, and OA were able to prevent the decrease in Abcd3 protein levels, which allows the measurement of peroxisomal mass, and in mRNA levels of Abcd1 and Abcd2, which encode for two transporters involved in peroxisomal β-oxidation. Thus, 7KC-induced side effects are associated with mitochondrial and peroxisomal dysfunction which can be inversed by natural compounds, thus supporting the hypothesis that the composition of the diet can act on the function of organelles involved in neurodegenerative diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Protective Effects of α-Tocopherol, γ-Tocopherol and Oleic Acid, Three Compounds of Olive Oils, and No Effect of Trolox, on 7-Ketocholesterol-Induced Mitochondrial and Peroxisomal Dysfunction in Microglial BV-2 Cells

Debbabi

Nury

Zarrouk

et al. 2016

IJMS

View full text Add to dashboard Cite

show abstract

“…Caco‐2 cells (ECACC, Salisbury, UK) were cultured in monolayers at 37 °C in a humidified atmosphere at 5% CO 2 (Incani and others ). At passage 45–60, cells were plated at a density of about 1 × 10 5 /mL and used when fully differentiated (21 d post seeding).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Effect of Natural Table Olives Phenolic Extract Against Oxidative Stress and Membrane Damage in Enterocyte‐Like Cells

Serreli

Incani

Atzeri

et al. 2017

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

The phenolic fraction of a naturally fermented cultivar of table olives, "Tonda di Cagliari," was investigated for the ability to protect Caco-2 cells against oxidative stress and membrane damage induced by tert-butyl hydroperoxyde (TBH). TBH exposure resulted in an alteration of cellular redox status, with an increase in reactive oxygen species (ROS) and a decrease in reduced glutathione (GSH) level. A loss of the epithelial integrity, as indicated by the decrease of the transepithelial electrical resistance value, was also observed over time, together with an intense lipid peroxidation process. The olives phenolic extract significantly counteracted ROS generation and subsequent alteration of monolayer integrity and membrane oxidative damage. The protective action of the extract is likely due to the scavenging ability of its main components, as hydroxytyrosol, oleuropein, and verbascoside among the secoiridoids and derivatives. Since olives phenolic compounds concentrate in the intestinal lumen, they may be a useful tool in the prevention of intestinal disorders related to oxidative damage.

show abstract

“…Moreover, the dysfunction of endothelial cells has been involved in the pathophysiology of metabolic diseases and DMT2 [90,91], and endothelial dysfunction is believed to contribute to the clinical expression of atherosclerosis and other complications of diabetes [92]. Overall, several findings have led to the hypothesis that HHV8 could be implicated in inducing some metabolic alterations, such as the increase of insulin binding and glucose uptake, which may be the initial event for activating the metabolic syndrome and diabetes [60,77,78,81,82,89,93]. It is commonly accepted that insulin hyper-secretion is considered a triggering cause of diabetes, and the discovery of the causes that are linked to insulin hyper-secretion is fundamental in the design of specific diabetes treatment [94,95].…”

Section: Hhv8 Prevalence In Chronic Diseasesmentioning

confidence: 99%

Human Herpesvirus 8 and Host-Cell Interaction: Long-Lasting Physiological Modifications, Inflammation and Related Chronic Diseases

2020

View full text Add to dashboard Cite

Oncogenic and latent-persistent viruses belonging to both DNA and RNA groups are known to cause serious metabolism alterations. Among these, the Human Herpesvirus 8 (HHV8) infection induces stable modifications in biochemistry and cellular metabolism, which in turn affect its own pathological properties. HHV8 enhances the expression of insulin receptors, supports the accumulation of neutral lipids in cytoplasmic lipid droplets and induces alterations in both triglycerides and cholesterol metabolism in endothelial cells. In addition, HHV8 is also known to modify immune response and cytokine production with implications for cell oxidative status (i.e., reactive oxygen species activation). This review underlines the recent findings regarding the role of latent and persistent HHV8 viral infection in host physiology and pathogenesis.

show abstract

Extra virgin olive oil phenolic extracts counteract the pro-oxidant effect of dietary oxidized lipids in human intestinal cells

Cited by 57 publications

References 49 publications

Protective Effects of α-Tocopherol, γ-Tocopherol and Oleic Acid, Three Compounds of Olive Oils, and No Effect of Trolox, on 7-Ketocholesterol-Induced Mitochondrial and Peroxisomal Dysfunction in Microglial BV-2 Cells

Protective Effects of α-Tocopherol, γ-Tocopherol and Oleic Acid, Three Compounds of Olive Oils, and No Effect of Trolox, on 7-Ketocholesterol-Induced Mitochondrial and Peroxisomal Dysfunction in Microglial BV-2 Cells

Antioxidant Effect of Natural Table Olives Phenolic Extract Against Oxidative Stress and Membrane Damage in Enterocyte‐Like Cells

Human Herpesvirus 8 and Host-Cell Interaction: Long-Lasting Physiological Modifications, Inflammation and Related Chronic Diseases

Contact Info

Product

Resources

About