EXTRA-LARGE G PROTEINs Interact with E3 Ligases PUB4 and PUB2 and Function in Cytokinin and Developmental Processes

Wang, Yiping; Wu, Yingying; Yu, Boying; Yin, Zhao; Xia, Yiji

doi:10.1104/pp.16.00816

Cited by 66 publications

(65 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Arabidopsis thaliana , there is one canonical Gα ( GPA1 ), one Gβ ( AGB1 ) and three Gγ genes ( AGGs ). In addition to GPA1 , there are three Gα‐like genes (named XLGs ) in Arabidopsis , which can interact with E3 ligases PUB4 and PUB2 and act in cytokinin signalling (Wang et al , ). The xlg1/2/3 triple knockout mutant showed reduced male fertility and short siliques in Arabidopsis (https://www.arabidopsis.org/).…”

Section: G‐protein Signallingmentioning

confidence: 99%

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Hussain

Shi

Scheben

et al. 2020

Plant Biotechnology Journal

View full text Add to dashboard Cite

Summary Fruit is seed‐bearing structures specific to angiosperm that form from the gynoecium after flowering. Fruit size is an important fitness character for plant evolution and an agronomical trait for crop domestication/improvement. Despite the functional and economic importance of fruit size, the underlying genes and mechanisms are poorly understood, especially for dry fruit types. Improving our understanding of the genomic basis for fruit size opens the potential to apply gene‐editing technology such as CRISPR/Cas to modulate fruit size in a range of species. This review examines the genes involved in the regulation of fruit size and identifies their genetic/signalling pathways, including the phytohormones, transcription and elongation factors, ubiquitin‐proteasome and microRNA pathways, G‐protein and receptor kinases signalling, arabinogalactan and RNA‐binding proteins. Interestingly, different plant taxa have conserved functions for various fruit size regulators, suggesting that common genome edits across species may have similar outcomes. Many fruit size regulators identified to date are pleiotropic and affect other organs such as seeds, flowers and leaves, indicating a coordinated regulation. The relationships between fruit size and fruit number/seed number per fruit/seed size, as well as future research questions, are also discussed.

show abstract

Section: G‐protein Signallingmentioning

confidence: 99%

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Hussain

Shi

Scheben

et al. 2020

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…Analysis of G-protein regulated pathways in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), maize (Zea mays), soybean (Glycine max), tomato (Solanum lycopersicum), and pea (Pisum sativum) has confirmed their roles in controlling multiple aspects of plant development, signaling, and stress responses (Misra et al, 2007;Sun et al, 2014Sun et al, , 2018Urano and Jones, 2014;Roy Choudhury and Pandey, 2015;Subramaniam et al, 2016;Bi et al, 2018;Liang et al, 2018;Wu et al, 2018). The most elaborate data are available from Arabidopsis where G-protein mutants exhibit altered phenotypes in response to almost all plant hormones at the physiological and molecular (large-scale omics) level (Pandey and Assmann, 2004;Pandey et al, 2006Pandey et al, , 2008Pandey et al, , 2009Pandey et al, , 2010Fan et al, 2008;Zhao et al, 2010;Alvarez et al, 2011;Chakravorty et al, 2011;Jin et al, 2013;Tsugama et al, 2013;Wang et al, 2017;Peng et al, 2018;Zhang et al, 2018b). Similarly, G-protein mutants in Arabidopsis (and other plants) show altered sensitivity to multiple abiotic stresses such as drought, temperature, salt, redox, ultraviolet, and high light (Zhang et al, 2011;He et al, 2013;Torres et al, 2013;Subramaniam et al, 2016;Lee et al, 2017;Kaur et al, 2018;Yu and Assmann, 2018).…”

mentioning

confidence: 99%

The Role of Gβ Protein in Controlling Cell Expansion via Potential Interaction with Lipid Metabolic Pathways

2019

View full text Add to dashboard Cite

Heterotrimeric G-proteins influence almost all aspects of plant growth, development, and responses to biotic and abiotic stresses in plants, likely via their interaction with specific effectors. However, the identity of such effectors and their mechanism of action are mostly unknown. While investigating the roles of different G-protein subunits in modulating the oil content in Camelina (Camelina sativa), an oil seed crop, we uncovered a role of Gb proteins in controlling anisotropic cell expansion. Knockdown of Gb genes causes reduced longitudinal and enhanced transverse expansion, resulting in altered cell, tissue, and organ shapes in transgenic plants during vegetative and reproductive development. These plants also exhibited substantial changes in their fatty acid and phospholipid profiles, which possibly leads to the increased oil content of the transgenic seeds. This increase is potentially caused by the direct interaction of Gb proteins with a specific patatin-like phospholipase, pPLAIIId. Camelina plants with suppressed Gb expression exhibit higher lipase activity, and show phenotypes similar to plants overexpressing pPLAIIId, suggesting that the Gb proteins are negative regulators of pPLAIIId. These results reveal interactions between the G-protein-mediated and lipid signaling/metabolic pathways, where specific phospholipases may act as effectors that control key developmental and environmental responses of plants.

show abstract

“…Other studies also support the ubiquitination of AUX/IAA, with which the activity of ARF might be regulated. Still, in the cytokinin signalling pathway, we found E3ligase of MMS21, and PUB2/4 [35,36], both of which regulate activity of ARR, which promotes the shoot regeneration.…”

Section: Discussionmentioning

confidence: 73%

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Dong

Li-zhi

et al. 2019

Genes

View full text Add to dashboard Cite

De novo shoot regeneration is one of the important manifestations of cell totipotency in organogenesis, which reflects a survival strategy organism evolved when facing natural selection. Compared with tissue regeneration, and somatic embryogenesis, de novo shoot regeneration denotes a shoot regeneration process directly from detatched or injured tissues of plant. Studies on plant shoot regeneration had identified key genes mediating shoot regeneration. However, knowledge was derived from Arabidopsis; the regeneration capacity is hugely distinct among species. To achieve a comprehensive understanding of the shoot regeneration mechanism from tree species, we select four genetic lines of Populus euphratica from a natural population to be sequenced at transcriptome level. On the basis of the large difference of differentiation capacity, between the highly differentiated (HD) and low differentiated (LD) groups, the analysis of differential expression identified 4920 differentially expressed genes (DEGs), which were revealed in five groups of expression patterns by clustering analysis. Enrichment showed crucial pathways involved in regulation of regeneration difference, including "plant hormone signal transduction", "cell differentiation", "cellular response to auxin stimulus", and "auxin-activated signaling pathway". The expression of nine genes reported to be associated with shoot regeneration was validated using quantitative real-time PCR (qRT-PCR). For the specificity of regeneration mechanism with P. euphratica, large amount of DEGs involved in "plant-pathogen interaction", ubiquitin-26S proteosome mediated proteolysis pathway, stress-responsive DEGs, and senescence-associated DEGs were summarized to possibly account for the differentiation difference with distinct genotypes of P. euphratica. The result in this study helps screening of key regulators in mediating the shoot differentiation. The transcriptomic characteristic in P. euphratica further enhances our understanding of key processes affecting the regeneration capacity of de novo shoots among distinct species.

show abstract

EXTRA-LARGE G PROTEINs Interact with E3 Ligases PUB4 and PUB2 and Function in Cytokinin and Developmental Processes

Cited by 66 publications

References 42 publications

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

The Role of Gβ Protein in Controlling Cell Expansion via Potential Interaction with Lipid Metabolic Pathways

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Contact Info

Product

Resources

About