Extra-Intestinal Fluoroquinolone-Resistant Escherichia coli Strains Isolated from Meat

Caruso, Giorgia; Giammanco, Anna; Cardamone, Cinzia; Oliveri, Giuseppa; Mascarella, Chiara; Capra, Giuseppina; Fasciana, Teresa

doi:10.1155/2018/8714975

Cited by 14 publications

(16 citation statements)

References 44 publications

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“…10 Various studies have reported that food animals, particularly poultry, may be a significant reservoir for transfer of antibiotic-resistant genes in clinical bacterial isolates. 11,12 E. coli ST131, also referred to as a "superbug", is responsible for community-acquired urinary tract infections (UTIs) and is resistant to fluoroquinolone and cephalosporin antibiotics. 13 The coexistence of antibiotic-resistant genes and virulence factors in carbapenem-resistant K. pneumoniae in Italy indicates the emergence of a new clone of K. pneumoniae, 14 which could pose a significant challenge in tackling antimicrobial resistance (AMR).…”

Section: Introductionmentioning

confidence: 99%

Detection of OXA-48 Gene in Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae from Urine Samples

Gurung¹,

Kafle²,

Dhungel³

et al. 2020

IDR

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Introduction: Resistance to carbapenem in Gram-negative bacteria is attributable to their ability to produce carbapenemase enzymes. The main objective of this study was to detect the presence of blaOXA-48 genes in carbapenem-resistant uropathogenic Escherichia coli and Klebsiella pneumoniae isolated from urine samples from patients attending Alka Hospital, Jawalakhel, Lalitpur, Nepal. Methods: A total of 1013 mid-stream urine samples were collected from patients with suspected urinary tract infection (UTI) between April and September 2018. The identified isolates underwent antibiotic susceptibility testing using the modified Kirby-Bauer discdiffusion method. Phenotypic carbapenemase production was confirmed by the modified Hodge test, and the blaOXA-48 gene was detected using conventional polymerase chain reaction. Results: Out of 1013 urine samples, 15.2% (154/1013) had bacterial growth. Among the isolates, 91.5% (141/154) were Gram-negative bacteria, and E. coli was the most common bacterial isolate (62.9%; 97/154), followed by K. pneumoniae 15.6% (24/154). Among 121 bacterial isolates (97 E. coli isolates and 24 K. pneumoniae isolates), 70.3% (52/121) were multidrug-resistant E. coli and 29.7% (22/121) were multidrug-resistant K. pneumoniae. In addition, 9.1% (11/121) were carbapenem resistant (both imipenem and meropenem resistant). Development of multidrug resistance and development of carbapenem resistance were significantly associated (p<0.05). Of the 11 carbapenem-resistant isolates, only seven were carbapenemase producers; of these, 28.6% (2/7) were E. coli, 72.4% (5/7) were K. pneumoniae and 42.8% (3/7) had the blaOXA-48 gene. Of the three bacterial isolates with the blaOXA-48 gene, 33.3% (1/3) were E. coli and 66.7% (2/3) were K. pneumoniae. Conclusion: One in ten isolates of E. coli and K. pneumoniae were carbapenem resistant. Among carbapenem-resistant isolates, one-third of E. coli and two-thirds of K. pneumoniae had the blaOXA-48 gene. OXA-48 serves as a potential agent to map the distribution of resistance among clinical isolates.

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Section: Introductionmentioning

confidence: 99%

Detection of OXA-48 Gene in Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae from Urine Samples

Gurung¹,

Kafle²,

Dhungel³

et al. 2020

IDR

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“…However, it is now evident that mutational-driven resistance is a weaker force compared to ARGs acquired via horizontal gene transfer (HGT) [ 6 ]. In pathogens, resistance genes can be acquired from diverse microbial habitats and taxa [ 5 , 7 ], including bacteria from pristine environments free of antibiotics introduced via human activities [ 8 ]. It is therefore important to assess which bacterial taxa and microbial biospheres are the predominant contributors to the evolution of resistance in pathogens [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska

Haan

Drown

2021

Microorganisms

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Monitoring antibiotic resistance genes (ARGs) across ecological niches is critical for assessing the impacts distinct microbial communities have on the global spread of resistance. In permafrost-associated soils, climate and human driven disturbances augment near-surface thaw shifting the predominant bacteria that shape the resistome in overlying active layer soils. This thaw is of concern in Alaska, because 85% of land is underlain by permafrost, making soils especially vulnerable to disturbances. The goal of this study is to assess how soil disturbance, and the subsequent shift in community composition, will affect the types, abundance, and mobility of ARGs that compose the active layer resistome. We address this goal through the following aims: (1) assess resistance phenotypes through antibiotic susceptibility testing, and (2) analyze types, abundance, and mobility of ARGs through whole genome analyses of bacteria isolated from a disturbance-induced thaw gradient in Interior Alaska. We found a high proportion of isolates resistant to at least one of the antibiotics tested with the highest prevalence of resistance to ampicillin. The abundance of ARGs and proportion of resistant isolates increased with disturbance; however, the number of ARGs per isolate was explained more by phylogeny than isolation site. When compared to a global database of soil bacteria, RefSoil+, our isolates from the same genera had distinct ARGs with a higher proportion on plasmids. These results emphasize the hypothesis that both phylogeny and ecology shape the resistome and suggest that a shift in community composition as a result of disturbance-induced thaw will be reflected in the predominant ARGs comprising the active layer resistome.

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“…Characterization of overlapping traits between avian pathogenic E. coli (APEC) and urinary pathogenic E. coli (UPEC), such as O-serogroups, virulence markers and assignments to phylogroups, encouraged this hypothesis and subsequent researches comparing APEC and human E. coli isolates including UPEC, neonatal meningitis E. coli (NMEC) and E. coli causing septicemia showed common molecular traits between human and avian strains [4, 6, 7]. While the presence of ST131 clone among E. coli isolates contaminating raw chicken meat wasn’t confirmed in the studies from Spain and Italy [8, 9], another survey from Spain found this specific clone in 7% of retail chicken samples [10]. Vincent et al also had identified E. coli ST131 from retail chicken samples in Canada but at a significant lower prevalence (0.4%) than in Spain [11].…”

Section: Introductionmentioning

confidence: 99%

Clonal diversity, virulence genes content and subclone status of Escherichia coli sequence type 131: comparative analysis of E. coli ST131 and non-ST131 isolates from Iran

et al. 2019

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Background The Escherichia coli sequence type 131 (ST131) is a well established clone causing significant extraintestinal infections worldwide. However, no studies have been reported the phenotypic and molecular traits of ST131 isolates in comparison to other clones of E. coli from Iran. So, we determined the differences between 69 ST131 strains collected during a one year surveillance study and 84 non-ST131 isolates, including 56 clinical fluoroquinolone resistant and 28 broiler colibacillosis isolates in terms of clonality and genetic background. Results ST131 isolates were associated with phylogroup B2 (68 out of 69 isolates, 98.4%), while clinical non-ST131 and fluoroquinolone resistant broiler isolates mainly belonged to phylogroup A. The highest virulence score was observed in ST131 clone, while they showed less diversity in virulence profiles than other clinical isolates. Almost all of the ST131 isolates (95.6%) were ExPEC and had the highest virulence scores, but their resistance scores were less than clinical non-ST131 isolates. Broiler isolates showed higher prevalence of ExPEC-associated virulence genes and CTX-M-G1/G9 resistance determinants as compared to clinical non-ST131 isolates. While bla OXA-48/NDM carbapenemases were mostly found in ST131 clone, resistance rate against ertapenem was higher among clinical non-ST131 strains. According to ERIC-based fingerprinting, the ST131 strains were more genetically similar, followed by non-ST131 and broiler isolates. Conclusions ST131 isolates possess the ability to make a balance between clonality and extent of resistance/virulence genes content, so this phenomenon gives a fitness advantage over other E. coli clones. The broilers E. coli population poses a potential zoonotic risk which could be transmitted to the community through the food chain. A number of factors are involved in the dissemination of and infections due to ST131 clone.

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Extra-Intestinal Fluoroquinolone-Resistant Escherichia coli Strains Isolated from Meat

Cited by 14 publications

References 44 publications

<p>Detection of OXA-48 Gene in Carbapenem-Resistant <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> from Urine Samples</p>

<p>Detection of OXA-48 Gene in Carbapenem-Resistant <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> from Urine Samples</p>

Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska

Clonal diversity, virulence genes content and subclone status of Escherichia coli sequence type 131: comparative analysis of E. coli ST131 and non-ST131 isolates from Iran

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