Extra- and intracellular hydrogen ion-selective microelectrode based on neutral carriers with extended pH response range in acid media

Chao, P; Ammann, D.; Oesch, U.; Simon, W.; Läng, Florian

doi:10.1007/bf00582318

Cited by 66 publications

(41 citation statements)

References 15 publications

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“…The voltage-and pH-sensitive microelectrodes were prepared as described previously (8). The pH electrode tip was filled with proton ionophore 1 mixture B (Fluka Chemical Corp., Ronkonkoma, NY), and back-filled with a pH 7 phosphate buffer (9). Electrodes were connected to high impedance electrometers (model FD-223; WPI, Inc., Sarasota, FL), the signal from the voltage microelectrode was subtracted electronically from the voltage of the pH electrode, and the amplified output of the subtraction circuitry was connected to the A-D converter of a computer.…”

Section: Measurements Of Radiolabeled Solutementioning

confidence: 99%

Specificity of Anion Exchange Mediated by Mouse Slc26a6

Jiang

Grichtchenko

Boron

et al. 2002

Journal of Biological Chemistry

143

149

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Recently, CFEX, the mouse orthologue of human SLC26A6, was localized to the brush border membrane of proximal tubule cells and was demonstrated to mediate Cl ؊ -formate exchange when expressed in Xenopus oocytes. The purpose of the present study was to examine whether mouse Slc26a6 can mediate one or more of the additional anion exchange processes observed to take place across the apical membrane of proximal tubule cells. The majority of Na ϩ , Cl Ϫ , and HCO 3 Ϫ filtered by the kidney is reabsorbed in the proximal tubule. Studies using isolated brush border vesicles and perfused tubules are consistent with the concept that a major fraction of Cl Ϫ entry across the apical membrane of proximal tubule cells occurs via Cl Ϫ -formate exchange and Cl Ϫ -oxalate exchange (1). The molecular identification of the transporter(s) responsible for these anion exchange activities has yet to be established with certainty. Recently, CFEX, the mouse orthologue of human SLC26A6 (2, 3), was demonstrated to be capable of mediating Cl Ϫ -formate exchange when expressed in Xenopus oocytes, and it was localized to the brush border membrane of proximal tubule cells by immunocytochemistry (4). Thus, Slc26a6 was proposed as a candidate to mediate Cl Ϫ -formate exchange in the proximal tubule (4).The purpose of the present study was to characterize the anion specificity of Slc26a6 in more detail and to test specifically the ability of Slc26a6 to mediate additional anion exchange processes known to take place across the apical membrane of proximal tubule cells, such as Cl Ϫ -oxalate exchange. We find that mouse Slc26a6 can mediate transport of oxalate, sulfate, and HCO 3 Ϫ in addition to Cl Ϫ and formate and can function in multiple exchange modes involving pairs of these anions, including Cl Ϫ -oxalate exchange. MATERIALS AND METHODS Measurements of Radiolabeled SoluteFluxes-Mouse Slc26a6 (CFEX) cDNA was subcloned into the Xenopus expression plasmid pGH19 (5) and heterologously expressed in Xenopus oocytes as described previously (4). For the experiments in this study, oocytes were injected with 50 nl of water (control) or Slc26a6 cRNA (25 ng), and transport was assayed 2 days later. For measurements of solute influx, oocytes were washed twice in 1 ml chloride-free buffer (98 mM potassium-gluconate/1.8 mM hemi-calcium-gluconate/1 mM hemi-magnesiumgluconate/5 mM Tris-Hepes, pH 7.5) and then incubated in 500 l of uptake solution (100 mM potassium-gluconate/5 mM Tris, pH adjusted to 7.5 with Hepes) containing the radiolabeled solutes to be tested ( 1 After 30-min incubation at room temperature, external isotope was removed by washing the oocytes three times with 1 ml of ice-cold chloride-free buffer. For efflux measurements, oocytes were first preloaded with radioisotope by incubating for 60 min in chloride-free buffer containing [ 14 C]oxalate. After three washes in ice-cold chloride-free buffer, the radioisotope content of oocytes was measured both initially and 15 min after suspension in solution containing test anions. Net efflux was ca...

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Section: Measurements Of Radiolabeled Solutementioning

confidence: 99%

Specificity of Anion Exchange Mediated by Mouse Slc26a6

Jiang

Grichtchenko

Boron

et al. 2002

Journal of Biological Chemistry

143

149

View full text Add to dashboard Cite

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“…9 After Simon 10 published information about tridodecylamine ionophore for hydrogen sensing, we synthesized various types of tertiary amine compound ionophores, and strived to understand the response characteristics of these ionophores. [11][12][13][14][15] Particularly, after our result concerning SCE based on the tribenzylamine (TBA) ionophore 16 was published, which had a wider response range than the electrodes with tridodecylamine (TDDA) ionophore, many types of aromatic tertiary amine compound ionophores were synthesized and studied. [17][18][19][20][21] We found that the response ranges shifted to an alkali range (toward pH 14) by extending the chain length between the phenyl ring and nitrogen in the ionophores, and were able to synthesize ionophores that exhibited a linear response up to pH 14.…”

Section: Introductionmentioning

confidence: 99%

“…[17][18][19][20][21] We found that the response ranges shifted to an alkali range (toward pH 14) by extending the chain length between the phenyl ring and nitrogen in the ionophores, and were able to synthesize ionophores that exhibited a linear response up to pH 14. 22 As can be seen in the various results published until now on aromatic and aliphatic ionophores, however, there have been no reports on ionophores that are particularly effective in a strong acidic solution.In the present work, amine compound ionophores that could respond linearly to strong acid were synthesized, which has been noted as a limit of SPM (solvent polymeric membrane) ionophores, [13][14][15][16][17][18][19][20][21][22] and response characteristic due to structural changes of the ionophores in strong acid were demonstrated. Especially, we examined the responses resulting from increasing numbers of phenyl rings in ionophores, which had not been previously studied.…”

mentioning

confidence: 99%

“…In the present work, amine compound ionophores that could respond linearly to strong acid were synthesized, which has been noted as a limit of SPM (solvent polymeric membrane) ionophores, [13][14][15][16][17][18][19][20][21][22] and response characteristic due to structural changes of the ionophores in strong acid were demonstrated. Especially, we examined the responses resulting from increasing numbers of phenyl rings in ionophores, which had not been previously studied.…”

mentioning

confidence: 99%

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A Hydrogen Ion-Selective Poly(aniline) Solid Contact Electrode Based on Dibenzylpyrenemethylamine Ionophore for Highly Acidic Solutions

et al. 2004

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Ion-selective electrodes (ISEs) are chemical sensors with the longest history, and still probably have the largest number of applications. 1 More recently, two types of potentiometric wiretype electrodes (WTEs), such as coated wire electrodes (CWEs) 2 and solid contact electrodes (SCEs), 3,4 have exhibited rapid development. In WTEs, a total elimination of the internal filling solution provides new advantages. Simplicity of design, lower costs, mechanical flexibility, the possibility of miniaturization and microfabrication has widened the applications for WTEs in the fields of medicine and biotechnology. In SCEs of these two advanced types of WTEs, after polymerization, these polymer-coated metal electrodes were dipped into a PVC cocktail membrane solution until a bead was formed. Especially, these SCEs based on the electropolymerization method of amino derivatives onto the metal surface produced very good results, such as stabilization of the base potential, reproducibility, best selectivity, wide response ranges and fast response time. 5,6 Thus, in recent years, there have been many reports on hydrogen ion-selective SCEs 7 and hydrogen ion-selective ionophores. 8 We have studied hydrogen ion-selective ionophores and SCEs in our laboratory. 9After Simon 10 published information about tridodecylamine ionophore for hydrogen sensing, we synthesized various types of tertiary amine compound ionophores, and strived to understand the response characteristics of these ionophores. [11][12][13][14][15] Particularly, after our result concerning SCE based on the tribenzylamine (TBA) ionophore 16 was published, which had a wider response range than the electrodes with tridodecylamine (TDDA) ionophore, many types of aromatic tertiary amine compound ionophores were synthesized and studied. [17][18][19][20][21] We found that the response ranges shifted to an alkali range (toward pH 14) by extending the chain length between the phenyl ring and nitrogen in the ionophores, and were able to synthesize ionophores that exhibited a linear response up to pH 14. 22 As can be seen in the various results published until now on aromatic and aliphatic ionophores, however, there have been no reports on ionophores that are particularly effective in a strong acidic solution.In the present work, amine compound ionophores that could respond linearly to strong acid were synthesized, which has been noted as a limit of SPM (solvent polymeric membrane) ionophores, [13][14][15][16][17][18][19][20][21][22] and response characteristic due to structural changes of the ionophores in strong acid were demonstrated. Especially, we examined the responses resulting from increasing numbers of phenyl rings in ionophores, which had not been previously studied. Additionally, other common pH response characteristics of all solid-state potentiometric sensors for hydrogen ion based on poly(aniline), covered with a plastic PVC membrane and activated by these ionophores, are presented and evaluated in terms of analytical applications, such as in artificial serum an...

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“…The first neutral carrier-based solvent polymeric membrane pH electrodes have been developed in Simon's laboratory, where tridodecylamine and other compounds containing basic nitrogen groups have been discovered as selective membrane compounds in pH electrodes. [17][18][19][20][21][22][23] Later, other authors continued this work, thus other various compounds (aliphatic, heteroaromatic and their derivatives) [24][25][26][27][28][29][30] have been studied as carriers of hydrogen ions. The results have been particularly good for tertiary amine compounds.…”

Section: Introductionmentioning

confidence: 99%