Extra- and intracellular accumulation of ultrasmall superparamagnetic iron oxides (USPIO) in experimentally induced abscesses of the peripheral soft tissues and their effects on magnetic resonance imaging

Gellißen, J.; Axmann, Ch.; Prescher, Andreas; Bohndorf, Klaus; Lodemann, Klaus‐Peter

doi:10.1016/s0730-725x(98)00206-9

Cited by 43 publications

(29 citation statements)

References 20 publications

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“…It is possible that other cell types with phagocytic capacity, such as neutrophils, may contribute to the increase in R2* value. 12 However, we have no reason to believe that the uptake would differ from previous studies and, indeed, phagocytosis into macrophages is 4 to 6 times greater with ferumoxytol than with ferumoxtran-10, a previously used USPIO agent. 23 Moreover, we have demonstrated that organs of the reticuloendothelial system, such as the liver and spleen, demonstrate marked increases in R2* values uptake of USPIOs by monocytes and macrophages.…”

Section: Discussionmentioning

confidence: 67%

“…10 These cells are predominantly macrophages, but neutrophils may also take up USPIO. 11,12 We have recently established that USPIO can be used to assess vascular cellular inflammation in patients with abdominal aortic aneurysms. 13 Histological examination of aneurysm tissue confirmed the colocalization and uptake of USPIO in areas with macrophage infiltration, and mural uptake of USPIO was associated with a 3-fold increase in the rate of abdominal aortic aneurysm expansion.…”

Section: Circ Cardiovasc Imagingmentioning

confidence: 99%

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Ultrasmall Superparamagnetic Particles of Iron Oxide in Patients With Acute Myocardial Infarction

Alam

Shah

Richards

et al. 2012

Circ: Cardiovascular Imaging

150

110

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Magnetic resonance imaging (MRI) is ideally suited for the serial examination of the heart because it is noninvasive, does not involve ionizing radiation, and has excellent soft tissue contrast and spatial resolution. Cardiac magnetic resonance, using T2-weighted imaging, has previously been used to detect Background-Inflammation following acute myocardial infarction (MI) has detrimental effects on reperfusion, myocardial remodelling, and ventricular function. Magnetic resonance imaging using ultrasmall superparamagnetic particles of iron oxide can detect cellular inflammation in tissues, and we therefore explored their role in acute MI in humans. Methods and Results-Sixteen patients with acute ST-segment elevation MI were recruited to undergo 3 sequential magnetic resonance scans within 5 days of admission at baseline, 24 and 48 hours following no infusion (controls; n=6) or intravenous infusion of ultrasmall superparamagnetic particles of iron oxide (n=10; 4 mg/kg). T2*-weighted multigradient-echo sequences were acquired and R2* values were calculated for specific regions of interest. In the control group, R2* values remained constant in all tissues across all scans with excellent repeatability (bias of −0.208 s Key Words: myocardial infarction ◼ inflammation ◼ magnetic resonance imaging ◼ ultrasmall superparamagnetic particles of iron oxide

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Section: Discussionmentioning

confidence: 67%

Section: Circ Cardiovasc Imagingmentioning

confidence: 99%

Ultrasmall Superparamagnetic Particles of Iron Oxide in Patients With Acute Myocardial Infarction

Alam

Shah

Richards

et al. 2012

Circ: Cardiovascular Imaging

150

110

View full text Add to dashboard Cite

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“…Therefore, it was able to be shown in animal experiments that non-infections synovial inflammation in arthritis models can be imaged using intravenously injected USPIO [173]. In a similar manner, the macrophage presence [174] and the increased extravasation of USPIO [175] in the edge region of bacterial soft tissue abscesses were able to be shown in T2w and T2*w MRI after i. v. USPIO application. Moreover, macrophages were able to be detected experimentally as markers of low-grade chronically inflamed activity of fat tissue in adipose mice after the i. v. injection of USPIO [176].…”

Section: Inflammation Infection and Adiposity Imagingmentioning

confidence: 85%

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Ittrich¹,

Peldschus²,

Raabe³

et al. 2013

Fortschr Röntgenstr

124

101

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Superparamagnetic iron oxide nanoparticles (SPIO) can be used to image physiological processes and anatomical, cellular and molecular changes in diseases. The clinical applications range from the imaging of tumors and metastases in the liver, spleen and bone marrow, the imaging of lymph nodes and the CNS, MRA and perfusion imaging to atherosclerotic plaque and thrombosis imaging. New experimental approaches in molecular imaging describe undirected SPIO trapping (passive targeting) in inflammation, tumors and associated macrophages as well as the directed accumulation of SPIO ligands (active targeting) in tumor endothelia and tumor cells, areas of apoptosis, infarction, inflammation and degeneration in cardiovascular and neurological diseases, in atherosclerotic plaques or thrombi. The labeling of stem or immune cells allows the visualization of cell therapies or transplant rejections. The coupling of SPIO to ligands, radio- and/or chemotherapeutics, embedding in carrier systems or activatable smart sensor probes and their externally controlled focusing (physical targeting) enable molecular tumor therapies or the imaging of metabolic and enzymatic processes. Monodisperse SPIO with defined physicochemical and pharmacodynamic properties may improve SPIO-based MRI in the future and as targeted probes in diagnostic magnetic resonance (DMR) using chip-based ?NMR may significantly expand the spectrum of in vitro analysis methods for biomarker, pathogens and tumor cells. Magnetic particle imaging (MPI) as a new imaging modality offers new applications for SPIO in cardiovascular, oncological, cellular and molecular diagnostics and therapy. Key Points: Citation Format:

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“…The change of MRI signal intensity was measured in whole ventricular wall in each transplanted heart. The MR signal intensity of the heart was normalized to that of the leg muscle, because USPIO particles were not readily taken up by muscular tissue, according to Gellissen et al 18 Dextran-coated USPIO particles were synthesized in our laboratory according to the method of Palmacci and Josephson 19 with slight modifications. 12 The MR relaxivities R 1 (spin-lattice relaxation rate constant, 1/T 2 , per mole of Fe in USPIO) and R 2 (spin-spin relaxation rate constant, 1/T 2 , per mole of Fe in USPIO) measured at 4.7 T were 3.8ϫ10 4 and 9.1ϫ10 4 (mol/L)/s, respectively.…”

Section: Mri Experimentsmentioning

confidence: 99%

Macrophage Accumulation Associated With Rat Cardiac Allograft Rejection Detected by Magnetic Resonance Imaging With Ultrasmall Superparamagnetic Iron Oxide Particles

et al. 2001

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Background-Acute cardiac allograft rejection continues to be the cause of graft loss and contributes to the morbidity and mortality after cardiac transplantation. In this study, we report a new method for detecting organ rejection in transplantation with an MR-based technique using dextran-coated ultrasmall superparamagnetic iron oxide (USPIO) particles. These particles (Ϸ27 nm in diameter) are known to shorten relaxation times in MRI experiments. Methods and Results-A new rat model of heterotopic heart and lung transplantation has been developed for MRI experiments. Allotransplantations (DA3 BN) were performed (nϭ8), with syngeneic transplantations (BN3 BN) serving as controls (nϭ8). MR images were obtained with a gradient echo method. At postoperative day 7, allotransplants developed moderate rejection as determined histopathologically. A significant reduction in MR signal intensity was observed after USPIO injection into rats with allotransplanted hearts. Syngeneic transplants showed no differences in MR signal intensity before and after USPIO injections. After injection of USPIO particles at postoperative day 6, a group of allotransplanted rats was treated with cyclosporin A (3 mg/kg). Animals treated with cyclosporin A for 7 days showed no reduction in MR signal intensity after USPIO reinjection at day 14, whereas animals treated for 4 days showed a significant decrease in MR signal intensity in the transplanted hearts indicative of acute graft rejection. Pathological analysis of these animals revealed that dextran-coated USPIO particles were taken up by the infiltrating macrophages that accumulated within the rejecting cardiac graft. Conclusions-This

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Extra- and intracellular accumulation of ultrasmall superparamagnetic iron oxides (USPIO) in experimentally induced abscesses of the peripheral soft tissues and their effects on magnetic resonance imaging

Cited by 43 publications

References 20 publications

Ultrasmall Superparamagnetic Particles of Iron Oxide in Patients With Acute Myocardial Infarction

Ultrasmall Superparamagnetic Particles of Iron Oxide in Patients With Acute Myocardial Infarction

Superparamagnetic Iron Oxide Nanoparticles in Biomedicine: Applications and Developments in Diagnostics and Therapy

Macrophage Accumulation Associated With Rat Cardiac Allograft Rejection Detected by Magnetic Resonance Imaging With Ultrasmall Superparamagnetic Iron Oxide Particles

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