External validation of blood eosinophils, FE_NOand serum periostin as surrogates for sputum eosinophils in asthma

Abstract

NTR1846 and NTR2364.

Improving the diagnosis of eosinophilic asthma

“…Only few studies have specifically looked at the accuracy of a combination of two or more biomarkers to detect eosinophilic airway inflammation [26,46,50]. Unfortunately, the results of these studies are not fully conclusive.…”

“…Unfortunately, the results of these studies are not fully conclusive. One study did not find an improvement in accuracy when combining blood eosinophils, FeNO, and periostin as compared to one of the single biomarkers [46], whereas another study did find a small enhancement in accuracy when combining FeNO and blood eosinophils [50]. Adding IgE to these models did not lead to any improvement of the results.…”

“…However, subsequent studies showed that the strength of the correlation between periostin and sputum eosinophilia, although significant, was relatively weak, and that neither serum nor sputum periostin was suitable for use as a surrogate marker of airway eosinophilia [43]. Wagener et al showed that serum periostin, when compared to blood eosinophils and FeNO, showed the lowest accuracy for distinguishing eosinophilic (sputum eosinophils ≥3%) from non-eosinophilic inflammation in two independent cohorts of patients with asthma [46].…”

The association between blood eosinophil count and benralizumab efficacy for patients with severe, uncontrolled asthma: subanalyses of the Phase III SIROCCO and CALIMA studies

“…These methods are invasive and require specialized training, and are not feasible for routine clinical practice. Of a range of different biomarkers, blood eosinophil counts offer the greatest diagnostic accuracy for sputum eosinophilia, with increasing specificity as blood eosinophil counts increase 28,29 . However, given the natural variability of blood eosinophil counts (even within 24 hours) and their sensitivity to corticosteroids, low blood eosinophil counts may belie clinically significant eosinophilic airway inflammation 13,30,31 .…”

Effect of Coriolus versicolor glucan on the stimulation of cytokine production in sarcoma-180-bearing mice

“…The serum levels of IL-2, -4, -6, -10, -17A and IFN-α and -γ were measured by ELISA as previously described (17). Briefly, following the treatment period, mice were euthanized and blood (1.5 ml) was sampled from the eyeballs of the mice into a 2 ml eppendorf tube and centrifuged for 15 min at 1,372 x g at 4˚C.…”